5 documents found, page 1 of 1
Guiding stem cell tenogenesis by modulation of growth factor signaling and cell...
Teixeira, Simão P. B.; Pardo, Alberto; Bakht, Syeda M.; Gómez-Florit, Manuel; Reis, R. L.; Gomes, Manuela E.; Domingues, Rui Miguel AndradeTendon injuries and tendinopathies are increasingly prevalent health problems currently lacking effective treatments. Tissue engineering offers promising strategies to boost the low innate regenerative ability of tendons. Within this context, the simultaneous leveraging of both physical and biochemical cues by engineered scaffolding systems can be explored to promote a stronger tenogenic response from stem cell...
Combining magnetically-assisted and matrix-assisted 3D bioprinting for anisotro...
Bakht, Syeda M.; Pardo, Alberto; Reis, R. L.; Domingues, Rui Miguel Andrade; Gomes, Manuela E.A major obstacle in biofabrication is replicating the organization of the extracellular matrix and cellular patterns found in anisotropic tissues within bioengineered constructs. While magnetically-assisted 3D bioprinting techniques have the potential to create scaffolds that mimic natural biological structures, they currently lack the ability to accurately control the dispersion of magnetic substances within t...
3D biomimetic constructs steer stem cell commitment by synergistic modulation o...
Teixeira, Simão P. B.; Pardo, Alberto; Bakht, Syeda M.; Gómez-Florit, Manuel; Reis, R. L.; Gomes, Manuela E.; Domingues, Rui Miguel AndradeTendon diseases are prevalent health concerns for which current therapies present limited success, in part due to the intrinsically low regenerative ability of tendons. Therefore, tissue engineering presents a potential to improve this outcome. Here, we hypothesize that a concurrent control over both biophysical and biochemical stimuli will boost the tenogenic commitment of stem cells, thus promoting regenerati...
Human tendon-on-chip: unveiling the effect of core compartment-t cell spatiotem...
Bakht, Syeda M.; Pardo, Alberto; Gómez-Florit, Manuel; Caballero, David; Kundu, Subhas C; Reis, R. L.; Domingues, Rui Miguel Andrade; Gomes, Manuela E.The lack of representative in vitro models recapitulating human tendon (patho)physiology is among the major factors hindering consistent progress in the knowledge-based development of adequate therapies for tendinopathy.Here, an organotypic 3D tendon-on-chip model is designed that allows studying the spatiotemporal dynamics of its cellular and molecular mechanisms.Combining the synergistic effects of a bioactiv...
3D bioprinting of miniaturized tissues embedded in self-assembled nanoparticle-...
Bakht, Syeda M.; Gómez-Florit, Manuel; Lamers, Tara Helena; Reis, R. L.; Domingues, Rui M. A.; Gomes, Manuela E.The creation of microphysiological systems like tissue and organ-on-chip for in vitro modeling of human physiology and diseases is gathering increasing interest. However, the platforms used to build these systems have limitations concerning implementation, automation, and cost-effectiveness. Moreover, their typical plastic-based housing materials are poor recreations of native tissue extracellular matrix (ECM) ...