10 documents found, page 1 of 1
Inglês Inglês
da Fonseca, Beatriz Velosa; Mendes Veiga, Maria Isabel; Baptista, Vitória; Pernaute-Lau, Leyre; Osório, Nuno; Calçada, CarlaMalaria, a devastating illness remains a global health concern with an estimated 249 million malaria cases in 85 malaria-endemic countries around the world. Malaria elimination, challenged by drug resistance, requires strategic interventions that could be the implementation of antimalarials with selective actions on the different phases of the parasite life cycle. Of particular relevance is gametocytocidal drug...
Development and fabrication of a microfluidic device for mechanical characteriz...
Ferreira, Margarida Gomes; Freitas, Bruno; Baptista, Vitória; Pinho, Diana; Minas, Graça; Veiga, Maria Isabel; Faustino, Vera; Catarino, Susana OliveiraThis work reports the design and fabrication of a polymeric microfluidic device, with a hyperbolic contraction, to promote extensional flow and allow the single cell mechanical characterization, in vitro, of healthy and malaria-infected red blood cells (RBCs). The obtained results show an extensional flow profile of the velocity across the microchannel for both healthy and infected RBCs, while the ability of th...
Inglês Inglês
da Fonseca, Beatriz Velosa; Mendes Veiga, Maria Isabel; Baptista, Vitória; Pernaute-Lau, Leyre; Osório, Nuno; Calçada, CarlaMalaria, a devastating illness remains a global health concern with an estimated 249 million malaria cases in 85 malaria-endemic countries around the world. Malaria elimination, challenged by drug resistance, requires strategic interventions that could be the implementation of antimalarials with selective actions on the different phases of the parasite life cycle. Of particular relevance is gametocytocidal drug...
Optical spectrophotometry as a promising method for quantification and tage dif...
Baptista, Vitória; Silva, Miguel; Ferreira, Gabriel M; Calçada, Carla; Minas, Graça; Veiga, Maria Isabel; Catarino, Susana OliveiraMalaria is one of the most life-threatening infectious diseases worldwide, claiming half a million lives yearly. Prompt and accurate diagnosis is crucial for disease control and elimination. Currently used diagnostic methods require blood sampling and fail to detect low-level infections. At the symptomatic stage of infection, the parasites feed on red blood cells' (RBCs) hemoglobin, forming inert crystals, the ...
CMOS spectrophotometric microsystem for malaria detection
Ferreira, Gabriel Malheiro; Baptista, Vitória; Silva, Vitor; Veiga, Maria I.; Minas, Graça; Catarino, Susana OliveiraObjectives: Optical spectrophotometry has been explored to quantify Plasmodium falciparum malaria parasites at low parasitemia, with potential to overcome the limitations of detection in the current diagnostic methods. This work presents the design, simulation and fabrication of a CMOS microelectronic detection system to automatically quantify the presence of malaria parasites in a blood sample. Methods: The de...
Review of microdevices for hemozoin-based Malaria detection
Baptista, Vitória; Peng, Weng Kung; Minas, Graça; Veiga, Maria Isabel; Catarino, Susana OliveiraDespite being preventable and treatable, malaria still puts almost half of the world’s population at risk. Thus, prompt, accurate and sensitive malaria diagnosis is crucial for disease control and elimination. Optical microscopy and immuno-rapid tests are the standard malaria diagnostic methods in the field. However, these are time-consuming and fail to detect low-level parasitemia. Biosensors and lab-on-a-chip...
Acetic acid triggers cytochrome c release in yeast heterologously expressing hu...
Guedes, Joana P.; Baptista, Vitória; Cátia S. Pereira; Sousa, Maria João; Manon, Stéphen; Chaves, Susana R.; Côrte-Real, ManuelaProteins of the Bcl-2 protein family, including pro-apoptotic Bax and anti-apoptotic Bcl-xL, are critical for mitochondrial-mediated apoptosis regulation. Since yeast lacks obvious orthologs of Bcl-2 family members, heterologous expression of these proteins has been used to investigate their molecular and functional aspects. Active Bax is involved in the formation of mitochondrial outer membrane pores, through ...
The future in sensing technologies for malaria surveillance: a review of hemozo...
Baptista, Vitória; Costa, Mariana S.; Calçada, Carla Sofia Martins; Silva, Miguel João Ferreira; Gil, José Pedro; Veiga, Maria IsabelEarly and effective malaria diagnosis is vital to control the disease spread and to prevent the emergence of severe cases and death. Currently, malaria diagnosis relies on optical microscopy and immuno-rapid tests; however, these require a drop of blood, are time-consuming, or are not specific and sensitive enough for reliable detection of low-level parasitaemia. Thus, there is an urge for simpler, prompt, and ...
Expansion of a Specific Plasmodium falciparum PfMDR1 Haplotype in Southeast Asi...
Calçada, Carla; Silva, Miguel; Baptista, Vitória; Thathy, Vandana; Silva-Pedrosa, Rita; Granja, Diana; Ferreira, Pedro Eduardo; Gil, José PedroArtemisinin-based combination therapies (ACTs) have been vital in reducing malaria mortality rates since the 2000s. Their efficacy, however, is threatened by the emergence and spread of artemisinin resistance in Southeast Asia. The Plasmodium falciparum multidrug resistance protein 1 (PfMDR1) transporter plays a central role in parasite resistance to ACT partner drugs through gene copy number variations (CNV) a...
Expansion of a specific plasmodium falciparum PfMDR1 Haplotype in southeast Asi...
Calçada, Carla Sofia Martins; Silva, Miguel; Baptista, Vitória; Thathy, Vandana; Pedrosa, Ana; Granja, Diana; Ferreira, Pedro Eduardo; Gil, José PedroArtemisinin-based combination therapies (ACTs) have been vital in reducing malaria mortality rates since the 2000s. Their efficacy, however, is threatened by the emergence and spread of artemisinin resistance in Southeast Asia. The Plasmodium falciparum multidrug resistance protein 1 (PfMDR1) transporter plays a central role in parasite resistance to ACT partner drugs through gene copy number variations (CNV) a...