3 documents found, page 1 of 1
Hitchhiking Trypanosoma cruzi minicircle DNA affects gene expression in human h...
Barbosa, Augusto Mello Simões; Argañaraz, Enrique Roberto; Costa, Ana Maria; Rosa, Ana de Cassia Oliveira Cardoso; Alves, Nivaldo PereiraThe horizontal transfer of Trypanosoma cruzi mitochondrial minicircle DNA to the genomes of naturally infected humans may play an important role in the pathogenesis of Chagas disease. Minicircle integrations within LINE-1 elements create the potential for foreign DNA mobility within the host genome via the machinery associated with this retrotransposon. Here we document integration of minicircle DNA fragments i...
Integration of Trypanosoma cruzi kDNA minicircle sequence in the host genome ma...
Barbosa, Augusto Mello Simões; Barros, Ana Maria; Nitz, Nadjar; Argañaraz, Enrique Roberto; Teixeira, Antonio Raimundo Lima CruzABSTRACT: Integration of kDNA sequences within the genome of the host cell shown by PCR amplification with primers to the conserved Trypanosoma cruzi kDNA minicircle sequence was confirmed by Southern hybridization with specific probes. The cells containing the integrated kDNA sequences were then perpetuated as transfected macrophage subclonal lines. The kDNA transfected macrophages expressed membrane antigens ...
Current millennium biotechniques for biomedical research on parasites and host-...
Teixeira, Antonio Raimundo Lima Cruz; Barbosa, Augusto Mello Simões; Faudry, Eric; Lozzi, Silene de Paulino; Argañaraz, Enrique RobertoThe development of biotechnology in the last three decades has generated the feeling that the newest scientific achievements will deliver high standard quality of life through abundance of food and means for successfully combating diseases. Where the new biotechnologies give access to genetic information, there is a common belief that physiological and pathological processes result from subtle modifications of ...