34 documents found, page 1 of 4
Translational control of the human erythropoietin expression via an upstream op...
Ferrão, José; Onofre, Claudia; Barbosa, Cristina; Romão, LuísaCellular stress activates an integrated stress response, which includes rapid changes in global and gene-specific translation. Translational regulation of specific transcripts mostly occurs at mRNA translation initiation and is mediated via different cis-acting elements present in the mRNA 5’ untranslated region (5’UTR), such as upstream open reading frames (uORFs). uORFs modulate translation of the main ORF by...
The mechanism through which translation-termination codons are recognized as pr...
Onofre, Claudia; Menezes, Juliane; Peixeiro, Isabel; Barbosa, Cristina; Romão, LuísaAbout one third of the gene mutations found in human genetic disorders, including cancer, result in premature termination codons (PTCs) and the rapid degradation of their mRNAs by nonsense-mediated decay (NMD). NMD controls the quality of eukaryotic gene expression. The strength of the NMD response appears to reflect multiple determinants on a target mRNA. We have reported that human mRNAs with a PTC in close p...
The mechanism through which nonsense mutations are recognized as premature tran...
Onofre, Claudia; Menezes, Juliane; Peixeiro, Isabel; Barbosa, Cristina; Romão, LuísaAbout one third of the gene mutations found in human genetic disorders, including cancer, result in premature termination codons (PTCs) and the rapid degradation of their mRNAs by nonsense-mediated decay (NMD). NMD controls the quality of eukaryotic gene expression. The strength of the NMD response appears to reflect multiple determinants on a target mRNA. We have reported that human mRNAs with a PTC in close p...
The interface between mRNA translation and nonsense-mediated decay in AUG-proxi...
Onofre, Cláudia; Menezes, Juliane; Duarte, Andreia; Peixeiro, Isabel; Barbosa, Cristina; Costa, Paulo; Romão, LuísaNonsense-mediated mRNA decay (NMD) is a surveillance pathway that recognizes and selectively degrades mRNAs carrying premature termination codons (PTCs). In addition, several studies have also implicated NMD in the regulation of steady-state levels of physiological mRNAs, and examples of natural NMD targets are transcripts containing upstream short open reading frames or long 3’ untranslated regions. The streng...
Regulation of the human erythropoietin expression via an upstream open reading ...
Onofre, Cláudia; Ferrão, José; Barbosa, Cristina; Romão, LuísaCellular stress activates an integrated stress response, which includes rapid changes in global and gene-specific translation. Translational regulation of specific transcripts mostly occurs at mRNA translation initiation and is mediated via different cis-acting elements present in the mRNA 5’ untranslated region (5’UTR); these elements include upstream open reading frames (uORFs). uORFs modulate translation of ...
The interaction between mRNA translation and nonsense-mediated decay in AUG-pro...
Onofre, Cláudia; Menezes, Juliane; Peixeiro, Isabel; Barbosa, Cristina; Romão, LuísaNonsense-mediated mRNA decay (NMD) is a surveillance pathway that recognizes and rapidly degrades mRNAs containing premature termination codons (PTCs). Although NMD has been intensively studied, it is still poorly understood how NMD discriminates between PTCs and normal stop codons. The unified model for NMD proposes that the decision of NMD triggering is the outcome of the competition between the cytoplasmic p...
The interplay between mRNA translation and nonsense-mediated decay in transcrip...
Onofre, Cláudia; Menezes, Juliane; Peixeiro, Isabel; Barbosa, Cristina; Romão, LuísaMammalian nonsense-mediated mRNA decay (NMD) is a splicing- and translation-dependent surveillance pathway that recognizes and selectively degrades mRNAs carrying premature termination codons (PTCs). In addition, several studies have also implicated NMD in the regulation of steady-state levels of physiological mRNAs, and examples of natural NMD targets are transcripts containing upstream short open reading fram...
How mRNA translation is involved in modulating nonsense-mediated decay in trans...
Onofre, Claudia; Menezes, Juliane; Peixeiro, Isabel; Costa, Nuno; Barbosa, Cristina; Romão, LuísaBeyond its well-known hematopoietic action, erythropoietin (EPO) has diverse cellular effects in non-hematopoietic tissues. For example, in cases of tissue injury, such as cardiac ischemia or acute myocardial infarct, EPO expression increases locally, providing a cardioprotective effect. Cellular stress activates an integrated stress response, which includes rapid changes in global and gene-specific translation...
Translational control of the human erythropoietin via an upstream open reading ...
Onofre, Claudia; Barbosa, Cristina; Romão, LuísaBeyond its well-known hematopoietic action, erythropoietin (EPO) has diverse cellular effects in non-hematopoietic tissues. For example, in cases of tissue injury, such as cardiac ischemia or acute myocardial infarct, EPO expression increases locally, providing a cardioprotective effect. Cellular stress activates an integrated stress response, which includes rapid changes in global and gene-specific translation...
How mRNA translation is involved in modulating nonsense-mediated decay in trans...
Onofre, Cláudia; Menezes, Juliane; Peixeiro, Isabel; Costa, Nuno; Barbosa, Cristina; Romão, LuísaNonsense-mediated mRNA decay (NMD) is a surveillance pathway that recognizes and selectively degrades mRNAs carrying premature termination codons (PTCs). In addition, several studies have also implicated NMD in the regulation of steady-state levels of physiological mRNAs, and examples of natural NMD targets are transcripts containing upstream short open reading frames or long 3’ untranslated regions. The streng...