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In vitro bioaccessibility of macro and trace elements in biofortified and conve...

Barbosa, Vera; Anacleto, Patrícia; Maulvault, Ana Luísa; Oliveira, Helena; Marmelo, Isa; Ferraris, Francesca; Raggi, Andrea; Ventura, Marta

Biofortification is a promising strategy to improve the nutrient profile of farmed fish but requires consideration of the nutrient bioaccessible fraction. In this study, the in vitro bioaccessibility of macro and trace elements was investigated in biofortified and conventional farmed gilthead seabream and common carp, also taking into account the effect of cooking (by steaming). Biofortification enhanced iodine...


Screening of Distal Symmetric Polyneuropathy in Hospitalized Diabetic Patients

Gonçalves, Helena; Lopes, Ana; Costa, Andreia; Calvão, Joana; Fonseca, Liliana; Gonçalves, João; Branco, Catarina; Martins, Raquel; Barbosa, Vera

Introduction: Distal symmetric polyneuropathy (DSPN) isa frequent, catastrophic and underdiagnosed complication ofdiabetes mellitus. The authors have hypothesized that hospitalization could be an opportunity to screen DSPN and alsopretend to compare two different diagnostic methods.Material and Methods: This was a prospective and multicentre study that enrolled diabetic patients admitted consecutively to intern...


Physicochemical properties of iodine and selenium biofortified Sparus aurata an...

Barbosa, Vera; Camacho, Carolina; Oliveira, Helena; Anacleto, Patrícia; Maulvault, Ana Luisa; Delgado, Inês; Ventura, Marta; Dias, Jorge; Ribeiro, Laura

Fish biofortification with natural ingredients like iodine-rich macroalgae and selenized-yeast is an excellent strategy to enhance the nutritional quality of farmed fish. This study aimed to assess the effect of frozen storage during 12-months on physicochemical quality of biofortified seabream (Sparus aurata) and carp (Cyprinus carpio). Frozen storage reduced iodine content in biofortified seabream fillets (17...


Enriched feeds with iodine and selenium from natural and sustainable sources to...

Barbosa, Vera; Maulvault, Ana Luísa; Anacleto, Patrícia; Santosa, Marta; Mai, Mónica; Oliveira, Helena; Delgadoe, Inês; Coelho, Inês; Barata, Marisa

Developing tailor-made fortified farmed fish is a promising solution to overcome nutritional deficiencies and increase consumer confidence in these products. This study evaluated the supplementation of three fortified diets with I-rich seaweed and selenised-yeast on essential and toxic elements levels in gilthead seabream (Sparus aurata) and common carp (Cyprinus carpio). Fortified diets resulted in increased I...


Bioaccessibility of lipophilic and hydrophilic marine biotoxins in seafood: an ...

Alves, Ricardo N.; Rambla-Alegre, Maria; Braga, Ana Catarina; Maulvault, Ana L.; Barbosa, Vera; Campàs, Mònica; Reverté, Laia; Flores, Cintia

This study aimed to assess the bioaccessibility of different marine biotoxins in naturally contaminated shellfish and fish gonads using an in vitro digestion methodology. In general, hydrophilic toxins (domoic acid, paralytic shellfish poisoning toxins and tetrodotoxins) showed higher bioaccessibility than lipophilic ones (okadaic acid and azaspiracids). The bioaccessibility of toxins from the okadaic acid grou...


Paralytic shellfish toxins and ocean warming: bioaccumulation and ecotoxicologi...

Barbosa, Vera; Santos, Marta; Anacleto, Patrícia; Maulvault, Ana Luísa; Pousão-Ferreira, Pedro; Reis Costa, Pedro; Marques, António

Warmer seawater temperatures are expected to increase harmful algal blooms (HABs) occurrence, intensity, and distribution. Yet, the potential interactions between abiotic stressors and HABs are still poorly understood from ecological and seafood safety perspectives. The present study aimed to investigate, for the first time, the bioaccumulation/depuration mechanisms and ecotoxicological responses of juvenile gi...


Bioaccessibility of lipophilic and hydrophilic marine biotoxins in seafood: an ...

Alves, Ricardo N.; Rambla-Alegre, Maria; Braga, Ana Catarina; Maulvault, Ana L.; Barbosa, Vera; Campàs, Mònica; Reverté, Laia; Flores, Cintia

This study aimed to assess the bioaccessibility of different marine biotoxins in naturally contaminated shellfish and fish gonads using an in vitro digestion methodology. In general, hydrophilic toxins (domoic acid, paralytic shellfish poisoning toxins and tetrodotoxins) showed higher bioaccessibility than lipophilic ones (okadaic acid and azaspiracids). The bioaccessibility of toxins from the okadaic acid grou...


Effects of steaming on contaminants of emerging concern levels in seafood

Barbosa, Vera; Luísa Maulvault, Ana; Alves, Ricardo N.; Kwadijk, Christian; Kotterman, Michiel; Tediosi, Alice; Fernández-Tejedor, Margarita

Seafood consumption is a major route for human exposure to environmental contaminants of emerging concern (CeCs). However, toxicological information about the presence of CeCs in seafood is still insufficient, especially considering the effect of cooking procedures on contaminant levels. This study is one among a few who evaluated the effect of steaming on the levels of different CeCs (toxic elements, PFCs, PAH...


Antidepressants in a changing ocean: Venlafaxine uptake and elimination in juve...

Luísa Maulvault, Ana; Santos, Lúcia H.M.L.M.; Camacho, Carolina; Anacleto, Patrícia; Barbosa, Vera; Alves, Ricardo; Pousão Ferreira, Pedro

The presence of antidepressants, such as venlafaxine (VFX), in marine ecosystems is increasing, thus, potentially posing ecological and human health risks. The inherent mechanisms of VFX uptake and elimination still require further understanding, particularly accounting for the impact of climate change-related stressors, such as warming and acidification. Hence, the present work aimed to investigate, for the fi...


Differential behavioural responses to venlafaxine exposure route, warming and a...

Luísa Maulvault, Ana; Santos, Lúcia H.M.L.M.; Paula, José Ricardo; Camacho, Carolina; Pissarra, Vasco; Fogaça, Fabiola; Barbosa, Vera; Alves, Ricardo

Antidepressants, such as venlafaxine (VFX), which are considered emerging environmental pollutants, are increasingly more present in the marine environment, and recent evidence suggest that they might have adverse effects on fish behaviour. Furthermore, altered environmental conditions associated to climate change (e.g. warming and acidification) can also have a determinant role on fish behaviour, fitness and s...


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