11 documents found, page 1 of 2
Spillover events of rabbit haemorrhagic disease virus 2 (recombinant GI.4P-GI.2...
Abade dos Santos, F.A.; Pinto, Andreia; Burgoyne, Thomas; Dalton, Kevin P.; Carvalho, Carina L.; Ramilo, David; Carneiro, Carla; Carvalho, TâniaRabbit haemorrhagic disease (RHD) is a major threat to domestic and wild European rabbits. Presently, in Europe, the disease is caused mainly by Rabbit haemorrhagic disease virus 2 (RHDV2/b or Lagovirus europaeus GI.2), the origin of which is still unclear, as no RHDV2 reservoir hosts were identified. After the RHDV2 emergence in 2010, viral RNA was detected in a few rodent species. Furthermore, RHDV2 was found...
Co‐infection by classic MYXV and ha‐MYXV in Iberian hare (Lepus granatensis) an...
Abade Dos Santos, F.A.; Dalton, Kevin P.; Carvalho, Carina L.; Casero, Maria; Alvarez, Angel L.; Parra, F.; Duarte, Margarida D.Myxomatosis is an emergent disease in the Iberian hare (Lepus granatensis). In this species, the disease is caused by a natural recombinant virus (ha-myxoma virus [MYXV]) identified for the first time in 2018 and has since been responsible for a large number of outbreaks in Spain and Portugal. The ha-MYXV, which harbours a 2.8 Kb insert-disrupting gene M009L, can also infect and cause disease in wild and domest...
A potential atypical case of rabbit haemorrhagic disease in a dwarf rabbit
Abade Dos Santos, F.A.; Magro, Carolina; Carvalho, Carina L.; Ruivo, Pedro; Duarte, Margarida D.; Peleteiro, Maria C.ABSTRACT - Rabbit haemorrhagic disease (RHD) is a highly contagious infectious disease of European wild and domestic rabbits. Rabbit haemorrhagic disease virus (RHDV, GI.1) emerged in 1986 in Europe, rapidly spreading all over the world. Several genotypes of RHDV have been recognised over time, but in 2010, a new virus (RHDV2/RHDVb, GI.2) emerged and progressively replaced the previous RHDV strains, due to the ...
A versatile qPCR for diagnosis of leporid gammaherpesvirus 5 using Evagreen® or...
Abade Dos Santos, F.A.; Carvalho, Carina L.; Peleteiro, Maria C.; Parra, Francisco; Duarte, M. D.ABSTRACT - In late 2019, the first herpesvirus in the genus Lepus, named leporid gammaherpesvirus 5 (LeHV-5) was described. At the time, herpetic typical lesions were observed in hares infected by the myxoma virus, which is known to induce immunosuppression. Though the real impact of LeHV-5 is still poorly understood, since it affects reproduction, it poses an additional threat to the already fragile population...
Harmless or Threatening? Interpreting the Results of Molecular Diagnosis in the...
Abade dos Santos, Fábio A.; Portela, Sara J.; Nogueira, Teresa; Carvalho, Carina L.; de Sousa, Rita; Duarte, Margarida D.Molecular methods, established in the 1980s, expanded and delivered tools for the detection of vestigial quantities of nucleic acids in biological samples. Nucleotide sequencing of these molecules reveals the identity of the organism it belongs to. However, the implications of such detection are often misinterpreted as pathogenic, even in the absence of corroborating clinical evidence. This is particularly sign...
Harmless or threatening? Interpreting the results of molecular diagnosis in the...
Abade dos Santos, F.A.; Portela, Sara J.; Nogueira, Teresa; Carvalho, Carina L.; Sousa, Rita de; Duarte, M. D.Molecular methods, established in the 1980s, expanded and delivered tools for the detection of vestigial quantities of nucleic acids in biological samples. Nucleotide sequencing of these molecules reveals the identity of the organism it belongs to. However, the implications of such detection are often misinterpreted as pathogenic, even in the absence of corroborating clinical evidence. This is particularly sign...
A quadruplex qPCR for detection and differentiation of classic and natural reco...
Abade Dos Santos, F.A.; Carvalho, Carina L.; Francisco, Parra; Dalton, Kevin P.; Peleteiro, Maria C.; Duarte, M. D.A natural recombinant myxoma virus (referred to as ha-MYXV or MYXV-Tol08/18) emerged in the Iberian hare (Lepus granatensis) and the European rabbit (Oryctolagus cuniculus) in late 2018 and mid-2020, respectively. This new virus is genetically distinct from classic myxoma virus (MYXV) strains that caused myxomatosis in rabbits until then, by acquiring an additional 2.8 Kbp insert within the m009L gene that disr...
A potential atypical case of rabbit haemorrhagic disease in a dwarf rabbit
Abade dos Santos, Fábio A.; Magro, Carolina; Carvalho, Carina L.; Ruivo, Pedro; Duarte, Margarida D.; Peleteiro, Maria C.Rabbit haemorrhagic disease (RHD) is a highly contagious infectious disease of European wild and domestic rabbits. Rabbit haemorrhagic disease virus (RHDV, GI.1) emerged in 1986 in Europe, rapidly spreading all over the world. Several genotypes of RHDV have been recognised over time, but in 2010, a new virus (RHDV2/RHDVb, GI.2) emerged and progressively replaced the previous RHDV strains, due to the lack of cro...
Recombinant myxoma virus infection associated with high mortality in rabbit far...
Abade Dos Santos, F.A.; Carvalho, Carina L.; Monteiro, Madalena; Carvalho, Paulo; Mendonça, Paula; Peleteiro, Maria C.; Duarte, Margarida D.ABSTRACT - Myxomatosis is an emergent disease in the Iberian hare, having been considered a rabbit disease for decades. Genome sequencing of the strains obtained from Iberian hares with myxomatosis showed these to be distinct from the classical ones that circulated in rabbits since the virus introduction in Europe, in 1952. The main genomic difference in this natural recombinant hare myxoma virus (ha-MYXV) is t...
Blood collection from the external jugular vein of Oryctolagus cuniculus algiru...
Abade dos Santos, Fabio A.; Carvalho, Carina L.; Peleteiro, M. Conceição; Gabriel, Sofia Isabel; Patrício, Rui; Carvalho, João; Cunha, Mónica V.In the last decades, the European wild rabbit, particularly the Oryctolagus cuniculus algirus, a keystone species in the Iberian Peninsula ecosystems, declined severely, raising concerns from the wildlife authorities. The hunting calendar in Portugal limits sampling collection to a narrow window of few months annually. Nevertheless, governmental wildlife protection laws allow live rabbit sampling for population...