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Screening Wild Birds for Tick-Borne Zoonotic Pathogens in Portugal

Loureiro, F; Mesquita, JR; Cardoso, L; Santos-Silva, S; Moreira, G; Bento, JT; Soeiro, V; Gonçalves, A; Silva, F; Barradas, PF; Matos, AC; Matos, M

Wild birds may be involved in the transmission of agents of infectious diseases, including zoonoses, a circumstance which raises a number of public and animal health issues. Migratory bird species play a significant role in the introduction of tick-borne pathogens to new geographic areas, contributing to the dissemination of various etiological agents. This preliminary study aimed to assess the occurrence of fo...


First Molecular Detection of Rickettsia conorii and Rickettsia helvetica in Tic...

Moreira, G; Moreira, RSS; Neves, FAD; Swiontek, V; Barradas, PF; Gomes-Gonçalves, S; Mesquita, JR

Vector-borne diseases, particularly those caused by Rickettsia species, pose a significant public health threat in Europe. Despite extensive research on tick-borne pathogens in various European countries, Luxembourg has yet not been studied for Rickettsia spp. in ticks infesting domestic animals. This study aimed to fill this gap by investigating the presence of Rickettsia spp. in Ixodes ricinus ticks collected...


Detection of biliary trematodes in sheep and goats from northern and central Po...

Santos-Silva, S; Cruz, AVS; Gomes da Silva, P; Bento, JT; Almeida, D; Barradas, PF; Cardoso, L; Mesquita, JR

Fasciolosis and dicrocoeliosis are severe parasitic infections that result in substantial economic damages to livestock farming globally as a consequence of reduced productivity and viscera condemnation. Molecular tests such as polymerase chain reaction (PCR) can detect Fasciola hepatica and Dicrocoelium dendriticum DNA with high sensitivity and specificity. In this study, we aimed to assess the presence of F. ...


Detection of tick-borne pathogens in Rhipicephalus bursa ticks collected from t...

Barradas, PF; Marques, J; Tavares, C; Brito, NV; Mesquita, JR

The Garrano is a semi-feral horse breed native to several mountains in the northern Iberian Peninsula. Despite being endangered, this unique breed of pony has managed to survive in the wild and continues to be selectively bred, highlighting their remarkable resilience and adaptability to harsh environments. Wildlife plays a critical role in the survival of tick vectors in their natural habitats and the transfer...


Molecular evidence of sporadic Coxiella burnetii excretion in sheep milk, centr...

Pires, H; Santos-Silva, S; Cruz, AVS; Cardoso, L; Lopes, AP; Pereira, MA; Nóbrega, C; Mega, AC; Santos, C; Cruz, R; Esteves, F; Vala, H; Matos, AC

Coxiella burnetii is the etiologic agent of Q fever, a worldwide zoonosis. Cattle, sheep and goats are considered the main reservoirs of the disease. Transmission to humans occurs mainly through the inhalation of infectious aerosols from milk, faeces, urine, and birth products from infected ruminants. In this study, a 2-year longitudinal approach was performed to ascertain the excretion of C. burnetii in bulk t...


Cervids as Sentinels for Rickettsia spp. in Portugal

Barradas, PF; Abrantes, AC; Vieira e Brito, N; Mesquita, JR

Cervids are highly exposed to ticks, however, their role in the life cycle of these rickettsiae has not been fully elucidated. Given the expanding distribution and growing population of deer species in Portugal, coupled with their direct and indirect interactions with humans during hunting, it becomes crucial to explore their role as sentinels and potential reservoirs of Rickettsia. The present investigation ai...


Diversity of Rickettsia spp. in ticks from wild mammals of Morocco and Mauritania

Santos-Silva, S; Santos, N; Boratyński, Z; Mesquita, JR; Barradas, PF

Ticks are known as vectors and reservoirs of rickettsiae and, wildlife vertebrate hosts as suitable dispersers of ticks contributing to the life cycle of rickettsial agents in nature. In the herein study, the presence of rickettsiae was investigated in ticks from wild mammals (Gerbillus and Jaculus, Vulpes rueppellii, Canis anthus, Felis lybica and Felis margarita) in Mauritania and Morocco. Morphological and m...


Assessment of the circulation of Dirofilaria immitis in dogs from northern Port...

Gomes-de-Sá, S; Santos-Silva, S; Moreira, AS; Barradas, PF; Amorim, I; Cardoso, L; Mesquita, JR

Dirofilariasis is a vector-borne disease frequent in many countries. Not only infected dogs, but also cats and wild canids (including wolves and foxes), represent important sources of infection for mosquitoes, which are the pathogen vectors. The disease is endemic in Mediterranean countries with increasing incidence in Italy, France, Greece and Spain, but limited epidemiological data is available from Portugal ...


Rickettsia massiliae circulation in sheep and attached Rhipicephalus sanguineus...

Mesquita, JR; Santos-Silva, S; de Sousa Moreira, A; Baptista, MB; Cruz, R; Esteves, F; Vala, H; Barradas, PF

Rickettsiosis is considered an emerging/re-emerging vector-borne disease that causes significant public health threats. Ticks are reservoirs and vectors of Rickettsia having a significant role in the transmission of rickettsiae. In Portugal, little is known about tick-borne Rickettsia species in sheep. Therefore, this study aimed to investigate rickettsiae infection in ticks and their sheep host from 27 farms i...


Dirofilaria immitis antigenemia and microfilaremia in Iberian wolves and red fo...

Gomes-de-Sá, S; Santos-Silva, S; Moreira, AS; Barradas, PF; Amorim, I; Cardoso, L; Mesquita, JR

Background: Dirofilaria immitis is a parasitic nematode endemic in the Mediterranean countries, which causes cardiopulmonary dirofilariosis in wild and domestic animals. Despite being recognized hosts of D. immitis, wild carnivores such as wolves and foxes are frequently disregarded when considering a potential role in the transmission of these zoonotic nematodes. In Portugal, studies available regarding D. imm...


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