10 documents found, page 1 of 1
Feasibility and Effectiveness of a 12-Week Concurrent Exercise Training on Phys...
Barros, D; Silva-Fernandes, A; Martins, S; Guerreiro, S; Magalhães, J; Carvalho, J; Marques, EA"Objective To examine the feasibility and effects of a 12-week exercise intervention on physical performance, muscular strength, and circulating myokines in frail individuals living in nursing homes. Design A cluster randomized, 2-period, 2-intervention crossover trial. Setting and Participants Frail residents of 9 nursing homes were randomly assigned to either 12 weeks of concurrent exercise training (n = 5, 2...
Urinary hydration biomarkers and water sources in older adults with neurocognit...
Queiros, C; Machado, FB; Barros, D; Sampaio, J; Sampaio, A; Barros, Renata; Moreira, Pedro; Ribeiro, O; Joana Carvalho; Padrão, PatríciaThe risk of dehydration in older adults with neurocognitive disorder (NCD) is controversial. The purpose of this study was to assess hydration status, its determinants, and water intake sources in older adults with NCD. A sample of 30 participants (>= 60 years) was included. Sociodemographic, clinical data and one 24-h urine sample were collected. Urinary osmolality, sodium, potassium, volume, and creatinine we...
Engineering Hydrogels with Affinity-Bound Laminin as 3D Neural Stem Cell Cultur...
Barros, D; Conde-Sousa, E; Gonçalves, AM; Han, WM; García, AJ; Amaral, IF; Pêgo, APLaminin incorporation into biological or synthetic hydrogels has been explored to recapitulate the dynamic nature and biological complexity of neural stem cell (NSC) niches. However, the strategies currently explored for laminin immobilization within three-dimensional (3D) matrices do not address a critical aspect influencing cell-matrix interactions, which is the control over laminin conformation and orientati...
An affinity-based approach to engineer laminin-presenting cell instructive micr...
Barros, D; Parreira, P; Furtado, J; Ferreira-da-Silva, F; Conde-Sousa, E; García, A; Martins, MCL; Amaral, IF; Pêgo, APLaminin immobilization into diverse biological and synthetic matrices has been explored to replicate the microenvironment of stem cell niches and gain insight into the role of extracellular matrix (ECM) on stem cell behavior. However, the site-specific immobilization of this heterotrimeric glycoprotein and, consequently, control over its orientation and bioactivity has been a challenge that has limited many of ...
Laminin-Inspired Cell-Instructive Microenvironments for Neural Stem Cells
Barros, D; Amaral, IF; Pêgo, APLaminin is a heterotrimeric glycoprotein with a key role in the formation and maintenance of the basement membrane architecture and properties, as well as on the modulation of several biological functions, including cell adhesion, migration, differentiation and matrix-mediated signaling. In the central nervous system (CNS), laminin is differentially expressed during development and homeostasis, with an impact o...
Synthetic matrix enhances transplanted satellite cell engraftment in dystrophic...
Han, W; Anderson, S; Mohiuddin, M; Barros, D; Nakhai, S; Shin, E; Amaral, IF; Pêgo, AP; García, A; Jang, YMuscle satellite cells (MuSCs) play a central role in muscle regeneration, but their quantity and function decline with comorbidity of trauma, aging, and muscle diseases. Although transplantation of MuSCs in traumatically injured muscle in the comorbid context of aging or pathology is a strategy to boost muscle regeneration, an effective cell delivery strategy in these contexts has not been developed. We engine...
Fibrin functionalization with synthetic adhesive ligands interacting with a6ß1 ...
Silva, J; Bento, AR; Barros, D; Laundos, TL; Sousa, SR; Quelhas, P; Sousa, MM; Pêgo, AP; Amaral, IFTo enhance fibrin hydrogel affinity towards pluripotent stem cell-derived neural stem/progenitor cells (NSPCs) and its capacity to support NSPC migration and neurite extension, we explored the tethering of synthetic peptides engaging integrin a6ß1, a cell receptor enriched in NSPCs. Six a6ß1 integrin ligands were tested for their ability to support integrin a6ß1-mediated adhesion of embryonic stem cell-derived ...
Biomimetic synthetic self-assembled hydrogels for cell transplantation
Barros, D; Amaral, IF; Pêgo, APThe development of three-dimensional matrices capable of recapitulating the main features of native extracellular matrix and contribute for the establishment of a favorable microenvironment for cell behavior and fate is expected to circumvent some of the main limitations of cell-based therapies. In this context, self-assembly has emerged as a promising strategy to engineer cell-compatible hydrogels. A wide numb...
Surface functionalization of polymeric nanospheres modulates macrophage activat...
Barros, D; Lima, S; Cordeiro-da-Silva, ATo characterize the production and application of carbohydrate functionalized poly(d,l-lactide-co-glycolide) (PLGA) nanospheres as immune-modulatory mediators in the treatment of visceral leishmaniasis (VL). Materials & methods: PLGA nanospheres were prepared by nanoprecipitation and surface functionalized with mannose, mannan or mannosamine moieties using a carbodiimide reaction. Flow cytometry and fluorescenc...
Crucial CD8(+) T-lymphocyte cytotoxic role in amphotericin B nanospheres effica...
Costa, LS; Silvestre, R; Barros, D; Cunha, J; Baltazar, M; Dinis-Oliveira, R; Cordeiro-da-Silva, AThis work aims to develop poly(d,l-lactide-co-glycolide) (PLGA)-nanospheres containing amphotericin B (AmB) with suitable physicochemical properties and anti-parasitic activity for visceral leishmaniasis (VL) therapy. When compared with unloaded-PLGA-nanospheres, the AmB-loaded PLGA-nanospheres displayed an increased particle size without affecting the polydispersity and its negative surface charge. AmB stabili...