8 documents found, page 1 of 1
Superior operational stability of immobilized l-asparaginase over surface-modif...
Almeida, Mafalda R.; Cristóvão, Raquel O.; Barros, Maria A.; Nunes, João C. F.; Boaventura, Rui A. R.; Loureiro, José M.; Faria, Joaquim L.Made available in DSpace on 2022-04-28T19:46:42Z (GMT). No. of bitstreams: 0 Previous issue date: 2021-12-01; Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP); Fundação para a Ciência e a Tecnologia; l-asparaginase (ASNase, EC 3.5.1.1) is an enzyme that catalyzes the l-asparagine hydrolysis into l-aspartic acid and ammonia, being mainly applied in pharmaceutical and food industries. However, some d...
Superior operational stability of immobilized L-asparaginase over surface-modif...
Almeida, Mafalda R.; Cristóvão, Raquel O.; Barros, Maria A.; Nunes, João C. F.; Boaventura, Rui A. R.; Loureiro, José M.; Faria, Joaquim L.L-asparaginase (ASNase, EC 3.5.1.1) is an enzyme that catalyzes the L-asparagine hydrolysis into L-aspartic acid and ammonia, being mainly applied in pharmaceutical and food industries. However, some disadvantages are associated with its free form, such as the ASNase short half-life, which may be overcome by enzyme immobilization. In this work, the immobilization of ASNase by adsorption over pristine and modifi...
Immobilization of L-asparaginase towards surface-modified carbon nanotubes
Tavares, Ana P. M.; Almeida, Mafalda R.; Cristóvão, Raquel O.; Barros, Maria A.; Nunes, João C. F.; Faria, Joaquim L.; Neves, Márcia C.; Freire, Mara G.L-asparaginase (LA) is an enzyme that catalyzes L-asparagine hydrolysis into L-aspartic acid and ammonia and is mainly applied in pharmaceutical and food industries. The LA currently commercialized for pharmaceutical purposes is produced from two main bacterial sources: recombinant Escherichia coli and Erwinia chrysanthemi. However, some disadvantages are associated with its free form, such as the shorter half-...
Immobilization of L-asparaginase towards surface-modified carbon nanotubes
Almeida, Mafalda R.; Cristóvão, Raquel O.; Barros, Maria A.; Nunes, João C.F.; Boaventura, Rui A. R.; Loureiro, José M.; Faria, Joaquim L.L-asparaginase (ASNase, EC 3.5.1.1) is an enzyme that catalyzes L-asparagine hydrolysis into L-aspartic acid and ammonia and is mainly applied in pharmaceutical and food industries [1]. The ASNase currently commercialized for pharmaceutical purposes is produced from two main bacterial sources: recombinant Escherichia coli and Erwinia chrysanthemi. However, some disadvantages are associated with its free form, s...
Development and characterization of a novel l-asparaginase/MWCNT nanobioconjugate
Cristovao, Raquel O.; Almeida, Mafalda R.; Barros, Maria A.; Nunes, Joao C. F.; Boaventura, Rui A. R.; Loureiro, Jose M.; Faria, Joaquim L.Made available in DSpace on 2020-12-12T01:38:47Z (GMT). No. of bitstreams: 0 Previous issue date: 2020-08-24; The enzyme l-asparaginase (ASNase) presents effective antineoplastic properties used for acute lymphoblastic leukemia treatment besides their potential use in the food sector to decrease the acrylamide formation. Considering their applications, the improvement of this enzyme's properties by efficient im...
Development and characterization of a novel l-asparaginase/MWCNT nanobioconjugate
Cristóvão, Raquel O.; Almeida, Mafalda R.; Barros, Maria A.; Nunes, João C. F.; Boaventura, Rui A. R.; Loureiro, José M.; Faria, Joaquim L.The enzyme L-asparaginase (ASNase) presents effective antineoplastic properties used for acute lymphoblastic leukemia treatment besides their potential use in the food sector to decrease the acrylamide formation. Considering their applications, the improvement of this enzyme's properties by efficient immobilization techniques is in high demand. Carbon nanotubes are promising enzyme immobilization supports, sinc...
Using carbon nanotubes for antileukemic drugs purification
Nunes, João C. F.; Cristóvão, Raquel O.; Barros, Maria A.; Almeida, Mafalda R.; Faria, Joaquim L.; Sampaio, Maria J.; Freire, Mara G.Nowadays, the average life expectancy is progressively increasing. Nevertheless, there are still several fatal diseases generally associated with aging, such as cancer, heart and neurological diseases. Biopharmaceuticals as nucleic-acid-based products, antibodies and recombinant proteins and enzymes are applied in order to overcome these age-related diseases. L-asparaginase (LA) is one of the most broadly used ...
Tailoring carbon nanotubes surface chemistry for the purification of antileukem...
Barros, Maria A.; Almeida, Mafalda R.; Faria, Joaquim L.; Sampaio, Maria J.; Tavares, Ana P. M.; Silva, Cláudia G.Biopharmaceuticals have been used to overcome fatal diseases related with aging. L-aspariginase (LA), in particular, has shown evidences of being efficient for leukemia therapy. The LA production and purification require several steps increasing the cost of the process. In this work, functionalized carbon nanotubes (CNTs) were studied as a cost-effective support to purify LA. It was shown that CNTs have strong ...