11 documents found, page 1 of 2
Selection of aptamers to target enterotoxigenic Escherichia coli infections
Campos, Ana Maria; Barros, Maria Margarida; Oliveira, Ricardo; Castro, Joana Isabel Reis; Araújo, Daniela Eira; Outor-Monteiro, Divanildo[Excerpt] Introduction Enterotoxigenic Escherichia coli (ETEC) is the leading cause of enteric infections in swine, resulting in significant costs for the industry (1 ). Initial adhesion to the intestinal epithelium relies on fimbriae, with F4 and F18 types being most associated with enteric infections. The traditional use of antibiotics for prevention and treatment is increasingly restricted due to rising anti...
An asset for food safety: the knowledge behind the physiological alterations in...
Barros, Maria Margarida; Campos, Ana Maria; Castro, Joana Isabel Reis; Oliveira, Ricardo; Araújo, Daniela Eira; Outor-Monteiro, DivanildoFoodborne pathogens represent a significant public health risk in both developed and developing countries. Among these pathogens, enterotoxigenic Escherichia coli (ETEC) is a major cause of diarrhea in humans and one of the leading causes of mortality in newly weaned pigs. The main sources of ETEC contamination include environments with poor hygiene and contaminated water, meat, cereals, and vegetables. Therefo...
In vitro selection of DNA aptamers against staphylococcal enterotoxin A
Oliveira, Ricardo; Pinho, Eva; Barros, Maria Margarida; Azevedo, Nuno Filipe; Almeida, Carina Manuela FernandesStaphylococcal enterotoxin A (SEA) is the most frequently reported in staphylococcal food poisoning (SFP) outbreaks. Aptamers are single-stranded nucleic acids that are seen as promising alternatives to antibodies in several areas, including diagnostics. In this work, systematic evolution of ligands by exponential enrichment (SELEX) was used to select DNA aptamers against SEA. The SELEX protocol employed magnet...
Application of DNA aptamers to block enterotoxigenic Escherichia coli toxicity ...
Barros, Maria Margarida; Castro, Joana Isabel Reis; Araújo, Daniela Eira; Oliveira, Ricardo; Campos, Ana Maria; Silva, Sónia CarinaEnterotoxigenic Escherichia coli (ETEC) is the major bacterial cause of diarrheal diseases in pigs, particularly at young ages, resulting in significant costs to swine farming. The pathogenicity of ETEC is largely dependent on the presence of fimbriae and the ability to produce toxins. Fimbriae are responsible for their initial adhesion to the intestinal epithelial cells, leading to the onset of infection. In p...
Emerging approaches for mitigating biofilm-formation-associated infections in f...
Araújo, Daniela Eira; Silva, Ana Rita; Fernandes, Rúben Rodrigues; Serra, Patrícia; Barros, Maria Margarida; Campos, Ana Maria; Oliveira, RicardoThe importance of addressing the problem of biofilms in farm, wild, and companion animals lies in their pervasive impact on animal health and welfare. Biofilms, as resilient communities of microorganisms, pose a persistent challenge in causing infections and complicating treatment strategies. Recognizing and understanding the importance of mitigating biofilm formation is critical to ensuring the welfare of anim...
Detecting enterotoxigenic Escherichia coli in animal production: method develop...
Barros, Maria Margarida; Oliveira, Ricardo; Castro, Joana Isabel Reis; Campos, Ana; Almeida, Gonçalo Nieto; Araújo, Daniela; Silva, Sónia CarinaSwine enteric colibacillosis is a disease characterized by an intestinal infection caused by enterotoxigenic Escherichia coli (ETEC). This infection mostly causes illness or death in neonatal and weaned pigs making it responsible for significant economic losses. Bacterial fimbriae (F4/F5/F6/F18/F41) are responsible for the adhesion to epithelial cells, and when both the immunological systems and the gut microbi...
Swine colibacillosis: global epidemiologic and antimicrobial scenario
Barros, Maria Margarida; Castro, Joana Isabel Reis; Araújo, Daniela Eira; Campos, Ana Maria; Oliveira, Ricardo; Silva, Sónia CarinaSwine pathogenic infection caused by Escherichia coli, known as swine colibacillosis, represents an epidemiological challenge not only for animal husbandry but also for health authorities. To note, virulent E. coli strains might be transmitted, and also cause disease, in humans. In the last decades, diverse successful multidrug-resistant strains have been detected, mainly due to the growing selective pressure o...
Aptamers for blocking enterotoxigenic Escherichia coli
Barros, Maria Margarida; Oliveira, Ricardo; Castro, Joana Isabel Reis; Araújo, Daniela Eira; Silva, Sónia Carina; Campos, Ana; Outor-Monteiro, DivanildoEnterotoxigenic Escherichia coli (ETEC) is the major cause of enteric infections in swine, resulting in significant costs for the swine industry. Among other virulence factors, fimbriae are essential for the initial adhesion of ETEC to the intestinal epithelial cells. In particular, the F4-type (K88) fimbriae are commonly associated with neonatal infections and most post-weaning diarrhoeal infections. These dis...
Detecting Enterotoxigenic Escherichia coli in animal production: method develop...
Barros, Maria Margarida; Campos, Ana Maria; Oliveira, Ricardo; Castro, Joana; Almeida, Gonçalo Nieto; Silva, Sónia; Monteiro, Divanildo OutorSwine enteric colibacillosis is a disease characterized by an intestinal infection caused by the colonization of enterotoxigenic Escherichia coli (ETEC). This infection mostly causes illness or death in neonatal and weaned pigs making it responsible for significant economic losses worldwide [1,2]. Bacterial fimbriae (F4/F5/F6/F18) allow the adhesion of the bacteria to epithelial cells, and when both the immunol...
Aptamers for blocking enterotoxigenic Escherichia coli
Barros, Maria Margarida; Oliveira, Ricardo; Campos, Ana Maria; Castro, Joana; Araújo, Daniela; Silva, Sónia; Monteiro, Divanildo Outor; Almeida, CarinaThe most common bacterial pathogen causing enteric infections in swine is enterotoxigenic Escherichiacoli (ETEC). ETEC-associated diseases, lead to acute diarrhoea and eventual death of the animal, resulting in significant costs to the swine industry [1]. The toxins/fimbriae produced areessentialfor theirpathogenicity. Fimbriae are responsible for the first adhesion of ETEC to the intestinal epithelial cells, g...