4 documents found, page 1 of 1
One-step purification of L-asparaginase from cell extracts using carbon xerogels
Cristóvão, Raquel O.; Barros, Rita A. M.; Marramaque, Teresa P.; Aguiar, Gonçalo G.; Almeida, Mafalda R.; Carabineiro, Sónia A. C.L-asparaginase (ASNase, EC 3.5.1.1) is an enzyme with wide applications in the pharmaceutical sector and food processing industries. It is mainly used as a biotherapeutic for treating Acute Lymphoblastic Leukemia (ALL) and to reduce acrylamide formation in starchy compounds. Despite its relevance, current purification methods for microbial enzymes involve complex and expensive techniques. To overcome this drawb...
Recent developments and challenges in the application of fungal laccase for the...
Magalhães, Flávia F.; Pereira, Ana F. S.; Cristóvão, Raquel O.; Barros, Rita A. M.; Faria, Joaquim L.; Silva, Cláudia G.; Freire, Mara G.According to the European Environment Agency, the textile industry is responsible for 20% of global water pollution due to dyeing and finishing products, thus facing severe environmental challenges. It is essential to design more biocompatible and sustainable treatment processes capable of removing dyes from industrial wastewater to fight this environmental hazard. Chemical industries must change traditional ch...
Immobilization and characterization of l-asparaginase over carbon xerogels
Barros, Rita A. M.; Cristóvão, Raquel O.; Carabineiro, Sónia A. C.; Neves, Márcia C.; Freire, Mara G.; Faria, Joaquim L.; Santos-Ebinuma, Valéria C.L-asparaginase (ASNase) is an aminohydrolase currently used in the pharmaceutical and food industries. Enzyme immobilization is an exciting option for both applications, allowing for a more straightforward recovery and increased stability. High surface area and customizable porosity make carbon xerogels (CXs) promising materials for ASNase immobilization. This work describes the influence of contact time, pH, a...
Unveiling the Influence of Carbon Nanotube Diameter and Surface Modification on...
Cristóvão, Raquel O.; Barros, Rita A. M.; Pinho, João G.; Teixeira, Lília S.; Neves, Márcia C.; Freire, Mara G.; Faria, Joaquim L.L-asparaginase (ASNase, EC 3.5.1.1) is an amidohydrolase enzyme known for its anti-cancer properties, with an ever-increasing commercial value. Immobilization has been studied to improve the enzyme’s efficiency, enabling its recovery and reuse, enhancing its stability and half-life time. In this work, the effect of pH, contact time and enzyme concentration during the ASNase physical adsorption onto pristine and...