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Production of Human Milk Fat Substitutes by Lipase-Catalyzed Acidolysis: Immobi...

Soares, Cleide M. F.; Barbosa, Milson S.; Santos, Samuel B.; Mattedi, Silvana; Lima, Álvaro S.; Pereira, Matheus M.; Tecelão, Carla

Human milk fat (HMF) triacylglycerols (TAGs) mainly contain palmitic acid esterified at the sn-2 position while oleic and other unsaturated fatty acids are located at positions sn-1,3. This study aimed at the production of HMF substitutes (HMFS) by lipase-catalyzed acidolysis of tripalmitin with oleic acid, in a solvent-free medium. Burkholderia cepacia lipase (BCL) was immobilized in silica (prepared with prot...


Production of human milk fat substitutes by lipase-catalyzed acidolysis: immobi...

Soares, Cleide M. F.; Barbosa, Milson S.; Santos, Samuel B.; Mattedi, Silvana; Lima, Álvaro S.; Pereira, Matheus M.; Tecelão, Carla

Human milk fat (HMF) triacylglycerols (TAGs) mainly contain palmitic acid esterified at the sn-2 position while oleic and other unsaturated fatty acids are located at positions sn-1,3. This study aimed at the production of HMF substitutes (HMFS) by lipase-catalyzed acidolysis of tripalmitin with oleic acid, in a solvent-free medium. Burkholderia cepacia lipase (BCL) was immobilized in silica (prepared with prot...


Selective partition of caffeine from coffee bean and guarana seed extracts usin...

Santos, Samuel B.; Reis, Igor A. O.; Silva, Camila P. C.; Campos, Andressa F.; Ventura, Sonia P. M.; Soares, Cleide M. F.; Lima, Alvaro S.

This work proposes the application of flexible alcoholic aqueous two-phase systems to manipulate the partition of caffeine. An optimization study was performed the caffeine partitioning towards top phase (60.0 wt% 2-propanol + 20.0 wt% K3PO4 at 303 K - K-caf = 3.4 and R-T = 89.1%) and bottom phase (60.0 wt% methanol + 17.5 wt% K2HPO4/KH2PO4 at 278 K - K-caf = 0.06 and R-B = 81.1%). These processes were evaluate...


Extraction and Recovery of Rutin from Acerola Waste using Alcohol-Salt-Based Aq...

Reis, Igor A. O.; Santos, Samuel B.; Pereira, Frances D. S.; Sobral, Carla R. S.; Freire, Mara G.; Freitas, Lisiane S.; Soares, Cleide M. F.

Extraction of rutin from acerola waste was investigated using alcohol-salt-based aqueous two-phase systems (ATPS). Initially, the partitioning was studied using model systems with pure and commercial rutin. The impact of the ATPS constituents and composition, initial amount of rutin, temperature and addition of electrolytes was evaluated. Rutin can be recovered either in the alcohol-or-salt-rich phase depending...


Increased significance of food wastes: selective recovery of added-value compounds

Reis, Igor A. O.; Santos, Samuel B.; Santos, Ludmila A.; Oliveira, Naiana; Freire, Mara G.; Pereira, Jorge F. B.; Ventura, Sónia P. M.

A single-step selective separation of two food additives was investigated using alcohol-salt aqueous two-phase systems (ATPS). The selective partitioning of two of the most used additives from a processed food waste material, vanillin and l-ascorbic acid, was successfully accomplished. The results obtained prove that alcohol-salt ATPS can be easily applied as cheaper processes for the selective recovery of valu...


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