7 documents found, page 1 of 1
DNA damage in circulating leukocytes measured with the comet assay may predict ...
Bonassi, Stefano; Ceppi, Marcello; Møller, Peter; Azqueta, Amaya; Milić, Mirta; Monica, Neri; Brunborg, Gunnar; Godschalk, Roger; Koppen, GudrunThe comet assay or single cell gel electrophoresis is the most common method used to measure strand breaks and a variety of other DNA lesions in human populations. To estimate the risk of overall mortality, mortality by cause, and cancer incidence associated with DNA damage, a cohort of 2,403 healthy individuals (25,978 person-years) screened in 16 laboratories using the comet assay between 1996 and 2016 was fo...
The hCOMET project: international database comparison of results with the comet...
Milić, Mirta; Ceppi, Marcello; Bruzzone, Marco; Azqueta, Amaya; Brunborg, Gunnar; Godschalk, Roger; Koppen, Gudrun; Langie, Sabine; Møller, PeterThe alkaline comet assay, or single cell gel electrophoresis, is one of the most popular methods for assessing DNA damage in the human population. One of the open issues concerning this assay is the identification of those factors that can explain the large inter-individual and inter-laboratory variation. International collaborative initiatives such as the hCOMET project - a COST Action launched in 2016 - repre...
The hCOMET project: International database comparison of results with the comet...
Milić, Mirta; Ceppi, Marcello; Bruzzone, Marco; Azqueta, Amaya; Brunborg, Gunnar; Godschalk, Roger; Koppen, Gudrun; Langie, Sabine; Møller, PeterThe alkaline comet assay, or single cell gel electrophoresis, is one of the most popular methods for assessing DNA damage in human population. One of the open issues concerning this assay is the identification of those factors that can explain the large inter-individual and inter-laboratory variation. International collaborative initiatives such as the hCOMET project - a COST Action launched in 2016 - represent...
DNA damage in circulating leukocytes measured with the comet assay may predict ...
Bonassi, Stefano; Ceppi, Marcello; Møller, Peter; Azqueta, Amaya; Milić, Mirta; Neri, Monica; Brunborg, Gunnar; Godschalk, Roger; Koppen, GudrunThe comet assay or single cell gel electrophoresis, is the most common method used to measure strand breaks and a variety of other DNA lesions in human populations. To estimate the risk of overall mortality, mortality by cause, and cancer incidence associated to DNA damage, a cohort of 2,403 healthy individuals (25,978 person-years) screened in 16 laboratories using the comet assay between 1996 and 2016 was fol...
Application of the comet assay in human biomonitoring: an hCOMET perspective
Azqueta, Amaya; Ladeira, Carina; Giovannelli, Lisa; Boutet-Robinet, Elisa; Bonassi, Stefano; Neri, Monica; Gajski, Goran; Duthie, SusanThe comet assay is a well-accepted biomonitoring tool to examine the effect of dietary, lifestyle, environmental and occupational exposure on levels of DNA damage in human cells. With such a wide range of determinants for DNA damage levels, it becomes challenging to deal with confounding and certain factors are interrelated (e.g. poor nutritional intake may correlate with smoking status). This review describes ...
Minimum Information for Reporting on the Comet Assay (MIRCA): recommendations f...
Møller, Peter; Azqueta, Amaya; Boutet-Robinet, Elisa; Koppen, Gudrun; Bonassi, Stefano; Milić, Mirta; Gajski, Goran; Costa, SolangeThe comet assay is a widely used test for the detection of DNA damage and repair activity. However, there are interlaboratory differences in reported levels of baseline and induced damage in the same experimental systems. These differences may be attributed to protocol differences, although it is difficult to identify the relevant conditions because detailed comet assay procedures are not always published. Here...
The European Registered Toxicologist (ERT): Current status and prospects for ad...
Wilks, Martin F.; Blaauboer, Bas J.; Schulte-Hermann, Rolf; Wallace, Heather M.; Galli, Corrado L.; Haag-Grönlund, Marie; Matović, VesnaFollowing its inception in 1994, the certification of European Registered Toxicologists (ERT) by EUROTOX has been recognized as ensuring professional competence as well as scientific integrity and credibility. Criteria and procedures for registration are contained in the ERT "Guidelines for Registration 2012". The register of ERT currently has over 1900 members. In order to continue the harmonisation of require...