10 documents found, page 1 of 1
Advancing bone tissue engineering research using bone-derived cells laden in bi...
Bastos, Ana Raquel FernandesBone health is critically challenged by issues such as slow fracture healing, osteoporosis, and bone infections. Osteoporosis, causing over 8.9 million fractures annually, burdens global healthcare system and is currently managed with bisphosphonates, bone grafts, and growth factors. However, these treatments frequently fall short due to the need for repeated interventions, limited effectiveness, and the diseas...
In vitro bone tissue engineering strategies: the relevance of cells and culturi...
Bastos, Ana Raquel Fernandes; Maia, F. Raquel; Oliveira, Joaquim M.; Reis, R. L.; Correlo, V. M.The most recent advances in bone tissue engineering (BTE) approaches step forward in the field of three-dimensional (3D) tissue models, enabling the development of more realistic tools to study bone disorders, such as osteoporosis. BTE field aims to mimic native bone tissue more truthfully, providing an appropriate environment for tissue regeneration and repair through the combination of 3D porous scaffolds, sp...
Hydroxyapatite/alginate/gellan gum inks with osteoconduction and osteogenic pot...
Bastos, Ana Raquel Fernandes; da Silva, Lucília Pereira; Maia, F. Raquel; Franco, Albina Ribeiro; Noro, Jennifer Martins; Silva, CarlaThere is a growing demand for engineered bone tissues custom-designed to match the patient-specific defect size and in vitro models for studying bone diseases and/or drug screening. Herein, we propose a bioprinted bone tissue construct using SaOs-2 cells within alginate/gellan gum/hydroxyapatite inks. Different ink formulations were developed with varying hydroxyapatite content and then evaluated for viscoelast...
Collagen membrane from bovine pericardium for treatment of long bone defect
Yamanaka, J. S.; Oliveira, A. C.; Bastos, Ana Raquel Fernandes; Fernandes, Emanuel Mouta; Reis, R. L.; Correlo, V. M.; Shimano, A. C.The treatment of bone regeneration failures has been constantly improved with the study of new biomaterials. Techgraft® is a collagen membrane derived from bovine pericardium, which has been shown to have biocompatibility and effectiveness in tissue repair. However, its use in orthopedics has not yet been evaluated. Therefore, the purpose of this study was to characterize a bovine pericardium collagen membrane ...
Pharmacological and non-pharmacological agents versus bovine colostrum suppleme...
Kydonaki, Eirini K.; Freitas, Laura; Reguengo, Henrique; Simón, Carlos Raposo; Bastos, Ana Raquel Fernandes; Fernandes, Emanuel MoutaOsteoporosis is defined by loss of bone mass and deteriorated bone microarchitecture. The present study compared the effects of available pharmacological and non-pharmacological agents for osteoporosis [alendronate (ALE) and concomitant supplementation of vitamin D (VD) and calcium (Ca)] with the effects of bovine colostrum (BC) supplementation in ovariectomized (OVX) and orchidectomized (ORX) rats. Seven-month...
Recent approaches towards bone tissue engineering
Maia, F. Raquel; Bastos, Ana Raquel Fernandes; Oliveira, J. M.; Correlo, V. M.; Reis, R. L.Bone tissue engineering approaches have evolved towards addressing the challenges of tissue mimetic requirements over the years. Different strategies have been combining scaffolds, cells, and biologically active cues using a wide range of fabrication techniques, envisioning the mimicry of bone tissue. On the one hand, biomimetic scaffold-based strategies have been pursuing different biomaterials to produce scaf...
Electroactive polyamide/cotton fabrics for biomedical applications
Bastos, Ana Raquel Fernandes; Silva, Lucília Pereira; Gomes, V. P.; Lopes, P. E.; Rodrigues, Luísa Cidália Guimarães; Reis, R. L.; Correlo, V. M.The latest advances on the development of wearables electrochemical sensors and biosensors has been revolutionizing healthcare, allowing a faster and specific diagnosis of pathological condition. The purpose of this work was to develop the first stage of wearable conductive based-textiles using natural (cotton) and synthetic (polyamide) fabrics composed of the conductive polypyrrole and polyaniline polymers. Co...
Lactoferrin-hydroxyapatite containing spongy-like hydrogels for bone tissue eng...
Bastos, Ana Raquel Fernandes; Silva, Lucília Pereira; Maia, F. Raquel; Pina, Sandra Cristina Almeida; Rodrigues, Tânia; Sousa, FilipaThe development of bioactive and cell-responsive materials has fastened the field of bone tissue engineering. Gellan gum (GG) spongy-like hydrogels present high attractive properties for the tissue engineering field, especially due to their wide microarchitecture and tunable mechanical properties, as well as their ability to entrap the responsive cells. Lactoferrin (Lf) and Hydroxyapatite (HAp) are bioactive fa...
Preparation of conductive fibers for biomedical applications
Bastos, Ana Raquel FernandesOs avanços na tecnologia dos biosensores tem vindo a revolucionar os cuidados de saúde, nomeadamente no diagnóstico de condições patológicas de forma rápida e eficaz, tornando o uso destes dispositivos uma realidade. O objetivo deste trabalho foi desenvolver tecidos condutores, sintéticos e naturais, compostos por poliamida (PA) ou algodão (CO), respetivamente, utilizando o polipirrole (PPy) e a polianilina (PA...
Gellan Gum Spongy-like Hydrogels Reinforced with Hydroxyapatite for Bone Tissue...
Maia, F. Raquel; Musson, D. S.; Naot, D.; Silva, Lucília Pereira; Bastos, Ana Raquel Fernandes; Costa, João B.; Oliveira, J. M.; Correlo, V. M.Bone tissue engineering with cell-laden-hydrogels has been attracting attention worldwide. In fact, they can resemble in some aspects the extracellular matrix, as high water content. In a forward thinking manner, our group engineered gellan gum (GG) spongy-like hydrogels where cells adhered and proliferated without the need of any modification. Moreover, we took advantage of these characteristics and combined i...