26 documents found, page 1 of 3
loysia citrodora extract as a chemopreventive agent against HPV16-induced lesio...
Medeiros-Fonseca, Beatriz; Faustino-Rocha, Ana I; Silva, Jessica; Pires, Maria João; Neuparth, Maria João; Vala, Helena; Vasconcelos-Nóbrega, CarmenAim: Aloysia citrodora has a long history of traditional use in treating various ailments. This study evaluated the in vivo chemopreventive efficacy and systemic toxicity of an extract of A. citrodora in a transgenic mouse model of HPV16 (human papillomavirus type 16)-induced cancer. Methods: The experiment involved six groups (n = 5): group 1 (G1, wild-type (WT), water), group 2 (G2, HPV, water), group 3 (G3, ...
Genetic Architecture of Congenital Hypogonadotropic Hypogonadism: Insights from...
Carriço, Josianne Nunes; Gonçalves, Catarina Inês; Al-Naama, Asma; Syed, Najeeb; Aragüés, José Maria; Bastos, Margarida; Fonseca, FernandoStudy question: What is the contribution of genetic defects in Portuguese patients with congenital hypogonadotropic hypogonadism (CHH)? Summary answer: Approximately one-third of patients with CHH were found to have a genetic cause for their disorder, with causal pathogenic and likely pathogenic germline variants distributed among 10 different genes; cases of oligogenic inheritance were also included. What is k...
Maturity-Onset Diabetes of the Young (MODY) in Portugal: Novel GCK, HNFA1 and H...
Alvelos, Maria I.; Gonçalves, Catarina I.; Coutinho, Eduarda; Almeida, Joana T.; Bastos, Margarida; Sampaio, Maria L.; Melo, Miguel; Martins, SofiaMaturity-onset diabetes of the young (MODY) is a frequently misdiagnosed type of diabetes, which is characterized by early onset, autosomal dominant inheritance, and absence of insulin dependence. The most frequent subtypes are due to mutations of the GCK (MODY 2), HNF1A (MODY 3), and HNF4A (MODY 1) genes. We undertook the first multicenter genetic study of MODY in the Portuguese population. The GCK, HNF1A, and...
Multi-step thermally induced transitions of ß-lactoglobulin - an in situ spectr...
Rodrigues, Rui M.; Claro, Bárbara; Bastos, Margarida; Pereira, Ricardo Nuno Correia; Vicente, A. A.; Petersen, Steffen B.An in-situ approach based in multiple spectroscopic techniques and benchmarked with DSC was used to characterise ß-Lg thermally-induced transitions. The methodology applied overcomes previously reported limitations, by ensuring similar experimental conditions in different determinations, non-aggregation conditions and allowing distinguishing between fluorescent variations due to collisional quenching and struct...
SLMP53-1 interacts with wild-type and mutant p53 DNA-binding domain and reactiv...
Gomes, A. S.; Ramos, Helena; Gomes, S.; Loureiro, Joana B.; Soares, Joana; Barcherini, Valentina; Monti, Paola; Fronza, GilbertoBackground Half of human cancers harbour TP53 mutations that render p53 inactive as a tumor suppressor. As such, reactivation of mutant (mut)p53 through restoration of wild-type (wt)-like function represents one of the most promising therapeutic strategies in cancer treatment. Recently, we have reported the (S)-tryptophanol-derived oxazoloisoindolinone SLMP53-1 as a new reactivator of wt and mutp53 R280K with i...
DIBMA nanodiscs keep α-synuclein folded
Adão, Regina; Cruz, Pedro F.; Vaz, Daniela C.; Fonseca, Fátima; Pedersen, Jannik Nedergaard; Ferreira-da-Silva, Frederico; Brito, Rui M. M.α-Synuclein (αsyn) is a cytosolic intrinsically disordered protein (IDP) known to fold into an α-helical structure when binding to membrane lipids, decreasing protein aggregation. Model membrane enable elucidation of factors critically affecting protein folding/aggregation, mostly using either small unilamellar vesicles (SUVs) or nanodiscs surrounded by membrane scaffold proteins (MSPs). Yet SUVs are mechanical...
A sexualização da mulher na publicidade das revistas de moda em Portugal: uma p...
Bastos, Margarida; Mota-Ribeiro, Silvana; Broega, A. C.Esta proposta procura dar conta do modo como diferentes aspetos das imagens publicitárias funcionam para construir aquilo que diversa/os autora/es definiram como “sexualização da mulher” ou “erotização do corpo feminino na publicidade”. A partir de uma perspetiva feminista que procura a desconstrução de manifestações comunicativas de forma crítica, apresenta-se o projeto teórico-metodológico que permite entreve...
Alcohols as molecular probes in ionic liquids: evidence for nanostructuration
Vaz, Inês C. M.; Bhattacharjee, Arijit; Rocha, Marisa A. A.; Coutinho, João A. P.; Bastos, Margarida; Santos, Luís M. N. B. F.A comprehensive study of the solution and solvation of linear alcohols (propan-1-ol, butan-1-ol and pentan-1-ol) in ionic liquids (ILs) is presented. The effect of the alkyl chain size of both alcohols and ILs (1-alkyl-3-methylimidazolium bis(trifluoromethylsulfonyl)imide, [CnC1im][NTf2], ionic liquid series) on the thermodynamic properties of solution and solvation was used to obtain insight into the interacti...
Alcohols as molecular probes in ionic liquids: evidence for nanostructuration
Vaz, Ines C. M.; Bhattacharjee, Arijit; Rocha, Marisa A. A.; Coutinho, Joao A. P.; Bastos, Margarida; Santos, Luis M. N. B. F.A comprehensive study of the solution and solvation of linear alcohols (propan-1-ol, butan-1-ol and pentan-1-ol) in ionic liquids (ILs) is presented. The effect of the alkyl chain size of both alcohols and ILs (1-alkyl-3-methylimidazolium bis(trifluoromethylsulfonyl)imide, [C(n)C(1)im][NTf2], ionic liquid series) on the thermodynamic properties of solution and solvation was used to obtain insight into the inter...
Gonadal Function in Turner Syndrome
Alves, Márcia; Bastos, Margarida; Almeida Santos, Teresa; Carrilho, FranciscoIntroduction: Turner syndrome is characterized by the absence, total or partial, of one X chromosome in females, being one of the most frequent chromosomal abnormalities. Diagnosis is made by karyotype. Turner syndrome manifestations include primary hypogonadism, before or after puberty (gonadal dysgenesis). The degree and extent of gonadal disfunction are variable.Objectives: We intended to assess clinical, ka...