7 documents found, page 1 of 1
Trypanosoma cruzi nucleoside triphosphate diphosphohydrolase 1 (TcNTPDase-1) bi...
Mariotini-Moura, Christiane; Bastos, Matheus Silva e; Castro, Felipe Freitas de; Trindade, Mellina Lanna; Vasconcellos, Raphael de SouzaPrevious work has suggested that Trypanosoma cruzi diphosphohydrolase 1 (TcNTPDase-1) may be involved in the infection of mammalian cells and serve as a potential target for rational drug design. In this work, we produced recombinant TcNTPDase-1 and evaluated its nucleotidase activity, cellular localization and role in parasite adhesion to mammalian host cells. TcNTPDase-1 was able to utilize a broad range of t...
Achievement of constitutive fluorescent pLEXSY-egfp Leishmania braziliensis and...
Bastos, Matheus Silva e; Souza, Luciana Ângelo de; Onofre, Thiago Souza; Almeida, Márcia Rogéria de; Bressan, Gustavo CostaGene reporter-fluorescent cells have emerged as alternative method for drug screening. Achievement of constitutive expression of fluorescent protein GFP by Leishmania braziliensis as alternative method for drug screening. L. braziliensis-GFP was generated using Leishmania tarentolae pLEXSY-egfp for constitutive expression of GFP. Fluorescent cells were selected and subjected to standardisation tests of anti-pro...
Leishmania infantum ecto-nucleoside triphosphate diphosphohydrolase-2 is an apy...
Vasconcellos, Raphael De Souza; Mariotini-Moura, Christiane; Gomes, Rodrigo Saar; Serafim, Tiago Donatelli; Firmino, Rafaela de CássiaVisceral leishmaniasis is an important tropical disease, and Leishmania infantum chagasi (synonym of Leishmania infantum) is the main pathogenic agent of visceral leishmaniasis in the New World. Recently, ecto-nucleoside triphosphate diphosphohydrolases (E-NTPDases) were identified as enablers of infection and virulence factors in many pathogens. Two putative E-NTPDases (∼70 kDa and ∼45 kDa) have been found in ...
Expressão das NTPDases 1 e 2 de Leishmania infantum chagasi em sistema bacteria...
Bastos, Matheus Silva eFundação de Amparo a Pesquisa do Estado de Minas Gerais; The Visceral Leishmaniasis (VL) represents an important global health problem in many countries including Brazil and it has seen a growing number of cases in humans and dogs. The dogs are being considered the main domestic reservoir responsible for maintaining the disease in the outskirts of large cities. The aim of this work is the study of NTPDase-1 and...
Expressão heteróloga e caracterização bioquímica e biológica da NTPDase-1 de Le...
Bastos, Matheus Silva eFundação de Amparo a Pesquisa do Estado de Minas Gerais; The Visceral Leishmaniasis (VL) represents an important global health problem in many countries including Brazil and it has seen a growing number of cases in humans and dogs. The dogs are being considered the main domestic reservoir responsible for maintaining the disease in the outskirts of large cities. The aim of this work is the study of Leishmania in...
Trypanosoma cruzi nucleoside triphosphate diphosphohydrolase 1 (TcNTPDase-1) bi...
Moura, Christiane Mariotini; Bastos, Matheus Silva e; Castro, Felipe Freitas de; Trindade, Mellina Lanna; Vasconcellos, Raphael de SouzaPrevious work has suggested that Trypanosoma cruzi diphosphohydrolase 1 (TcNTPDase-1) may be involved in the infection of mammalian cells and serve as a potential target for rational drug design. In this work, we produced recombinant TcNTPDase-1 and evaluated its nucleotidase activity, cellular localiza- tion and role in parasite adhesion to mammalian host cells. TcNTPDase-1 was able to utilize a broad range of...
Influence of ecto-nucleoside triphosphate diphosphohydrolase activity on Trypan...
Santos, Ramon de Freitas; Pôssa, Marcela Auxiliadora Souza; Bastos, Matheus Silva e; Guedes, Paulo Marcos da Matta; Almeida, Márcia Rogéria deBackground: The protozoan Trypanosoma cruzi is the causative agent of Chagas disease. There are no vaccines or effective treatment, especially in the chronic phase when most patients are diagnosed. There is a clear necessity to develop new drugs and strategies for the control and treatment of Chagas disease. Recent papers have suggested the ecto-nucleotidases (from CD39 family) from pathogenic agents as importa...