10 documents found, page 1 of 1

Resveratrol-loaded polydimethylsiloxane-silica hybrid materials: synthesis, cha...

In this work, hybrid materials within the polydimethylsiloxane-silica (PDMS-SiO2) system, synthesized via the sol-gel method, were developed and characterized for their potential to incorporate and release the bioactive compound resveratrol (RES). RES was incorporated into the materials with a high loading efficiency (>75%) using the rotary evaporator technique. This incorporation induced the amorphization of R...

3D sub-cellular localization of upconverting nanoparticles through hyperspectra...

Hyperspectral microscopy is an intriguing technique combining spectroscopy with optical microscopy that can be used to simultaneously obtain spectral and spatial information. The relevance of hyperspectral imaging in biomedical applications such as the monitoring of bioimaging agents, the identification of pathogens and cancerous cells, and the cellular uptake of nanoparticles has emerged recently, due to recen...

Coating of Magnetite Nanoparticles with Fucoidan to Enhance Magnetic Hypertherm...

Magnetic nanoparticles (NP), such as magnetite, have been the subject of research for application in the biomedical field, especially in Magnetic Hyperthermia Therapy (MHT), a promising technique for cancer therapy. NP are often coated with different compounds such as natural or synthetic polymers to protect them from oxidation and enhance their colloidal electrostatic stability while maintaining their thermal ...

Bacterial nanocellulose-hyaluronic acid microneedle patches for skin applicatio...

The aim of the present study was to develop innovative patches for dermo-cosmetic applications based on dissolvable hyaluronic acid (HA) microneedles (MNs) combined with bacterial nanocellulose (BC) as the back layer. HA was employed as an active biomacromolecule, with hydrating and regenerative properties and volumizing effect, whereas BC was used as support for the incorporation of an additional bioactive mol...

Desenvolvimento e estabelecimento de linha celular de blástula de Xenopus laevis

A redução do uso de experimentação animal tem-se tornado uma prioridade nos últimos anos. As metodologias in vitro, nomeadamente através da realização de experiências com linhas celulares, têm demonstrado constituir uma alternativa rápida, simples, de baixo custo e sensível à experimentação animal. O desenvolvimento destas metodologias é particularmente relevante no contexto de avaliação de risco (AR) de contam...

Macrophage metabolomics reveals differential metabolic responses to subtoxic le...

This study employed NMR metabolomics to characterize macrophage responses to subtoxic concentrations of silver nanoparticles (AgNPs ca. 30 nm) and ionic silver (Ag+), with a view to further elucidate their immunomodulatory activity at the cell metabolism level. Exposure to AgNPs caused RAW 264.7 macrophages to decrease intracellular glucose utilization, possibly due to interference with glycolytic enzymes, and ...

Pullulan microneedle patches for the efficient transdermal administration of in...

The present study reports the fabrication of dissolvable microneedle (MN) patches using pullulan (PL), a water-soluble polysaccharide with excellent film-forming ability, for the transdermal administration of insulin, envisioning the non-invasive treatment of diabetes. PL MNs patches were successfully prepared by micromoulding and revealed good thermal stability (Tdmax = 294 °C) and mechanical properties (>0.15...

Desenvolvimento e estabelecimento de linha celular de blástula de Xenopus laevis

A redução do uso de experimentação animal tem-se tornado uma prioridade nos últimos anos. As metodologias in vitro, nomeadamente através da realização de experiências com linhas celulares, têm demonstrado constituir uma alternativa rápida, simples, de baixo custo e sensível à experimentação animal. O desenvolvimento destas metodologias é particularmente relevante no contexto de avaliação de risco (AR) de contam...

Bridging a Gap between Cr(VI)-Induced Oxidative Stress and Genotoxicity in Lett...

Chromium(Cr) contamination in arable soils and irrigating water remains a priority, particularly due to the challenges posed to crop production and food safety. Long-term Cr(VI) effects remain less addressed than short-term ones, particularly regarding organspecific genotoxic profiles. Here we used the crop Lactuca sativa growing in a protected horticultural system and irrigated for 21 days with Cr(VI) (up to 2...

A pilot observational study on pets DNA damage