4 documents found, page 1 of 1
Grooved poly(lactide-co-trimethylene carbonate) substrates in tenogenic media m...
Ribeiro, S.; Pugliese, E.; Korntner, S. H.; Fernandes, E. M.; Gomes, Manuela E.; Reis, R. L.; O'Riordan, A.; Kearns, S.; Kelly, J. L.; Biggs, M.Tissue engineering strategies for tendon repair and regeneration rely heavily on the use of tendon derived cells. However, these cells frequently undergo phenotypic drift in vitro, which compromises their therapeutic potential. In order to maintain the phenotype of tendon derived cells in vitro, microenvironmental cues (biophysical, biochemical and/or biological in origin) have been used to better imitate the c...
Assessing the combined effect of surface topography and substrate rigidity in h...
Ribeiro, S.; Pugliese, E.; Korntner, S. H.; Fernandes, Emanuel Mouta; Gomes, Manuela E.; Reis, R. L.; O’Riordan, A.; Bayon, Y.; Zeugolis, D. I.The combined effect of surface topography and substrate rigidity in stem cell cultures is still under-investigated, especially when biodegradable polymers are used. Herein, we assessed human bone marrow stem cell response on aliphatic polyester substrates as a function of anisotropic grooved topography and rigidity (7 and 12 kPa). Planar tissue culture plastic (TCP, 3 GPa) and aliphatic polyester substrates wer...
Designing microenvironments for optimal outcomes in tissue engineering and rege...
Tsiapalis, D.; Ribeiro, S.; De Pieri, A.; Sallent, I.; Guillaumin, S.; Gaspar, D.; Korntner, S.; Bayon, Y.; Gomes, Manuela E.; Reis, R. L.Bone marrow mesenchymal stem cells have been extensively used for tissue engineering and regenerative medicine applications due to their ease of isolation and expansion and their ability to differentiate towards various lineages of mesodermal origin. Despite these properties, their clinical potential is often hampered by the simplicity of the in vitro environment and its inability to resemble the complex in viv...
Design and characterization of synthetic biodegradable films for musculoskeleta...
Ribeiro, S.; Novacek, V.; Fernandes, Emanuel Mouta; Gomes, Manuela E.; Reis, R. L.; Bayon, Y.; Zeugolis, D. I.To repair soft tissue, it is vital to ensure that the biomaterial is able to mimic the complex elasticity of the native tissue. It has been demonstrated that substrate stiffness has a huge influence on cellular growth, differentiation, motility and phenotype maintenance. The goal of the present study is to characterize extensively a set of polymeric films with variable mechanical profiles. A range of synthetic ...