19 documents found, page 1 of 2
Changing human hair fibre colour and shape from the follicle
Matamá, Maria Teresa Gonçalves Macedo; Costa, Cristiana Filipa Chaves; Fernandes, Bruno; Araújo, Rita; Cruz, Célia F.; Tortosa, FranciscoIntroduction: Natural hair curvature and colour are genetically determined human traits, that we inten tionally change by applying thermal and chemical treatments to the fibre. Presently, those cosmetic methodologies act externally and their recurrent use is quite detrimental to hair fibre quality and even to our health. Objectives: This work represents a disruptive concept to modify natural hair colour and cur...
Comparative analysis of transcriptomic responses to sub-lethal levels of six en...
Gil, Fátima N.; Gonçalves, Alina C.; Becker, Jörg D.; Viegas, Cristina A.Accidental spills and misuse of pesticides may lead to current and/or legacy environmental contamination and may pose concerns regarding possible risks towards non-target microbes and higher eukaryotes in ecosystems. The present study was aimed at comparing transcriptomic responses to effects of sub-lethal levels of six environmentally relevant pesticide active substances in the Saccharomyces cerevisiae eukaryo...
The regulator LdhR and the d-lactate dehydrogenase LdhA of Burkholderia multivo...
Silva, Inês N.; Ramires, Marcelo J.; Azevedo, Lisa A.; Guerreiro, Ana R.; Tavares, Andreia C.; Becker, Jörg D.; Moreira, Leonilde M.LysR-type transcriptional regulators (LTTR) are the most commonly found regulators in Burkholderia cepacia complex, comprising opportunistic pathogens causing chronic respiratory infections in cystic fibrosis (CF) patients. Despite LTTRs being global regulators of pathogenicity in several bacteria, few have been characterized in Burkholderia Here, we showed that gene ldhR of B. multivorans encoding a LTTR is co...
Glutamate receptor-like channels are essential for chemotaxis and reproduction ...
Ortiz-Ramírez, Carlos; Michard, Erwan; Simon, Alexander A.; Damineli, Daniel S. C.; Hernández-Coronado, Marcela; Becker, Jörg D.; Feijó, José A.Glutamate receptors are well characterized channels that mediate cell-to-cell communication during neurotransmission in animals. Nevertheless, information regarding their functional role in organisms without nervous systems is still limited. In plants, Glutamate Receptor-like (GLR) genes have been implicated in defence against pathogens, reproduction, control of stomata aperture and light signal transduction(1-...
Early programming of the oocyte epigenome temporally controls late prophase I t...
Navarro-Costa, Paulo; McCarthy, Alicia; Prudêncio, Pedro; Greer, Christina; Guilgur, Leonardo G.; Becker, Jörg D.; Secombe, Julie; Rangan, PrashanthOocytes are arrested for long periods of time in the prophase of the first meiotic division (prophase I). As chromosome condensation poses significant constraints to gene expression, the mechanisms regulating transcriptional activity in the prophase I-arrested oocyte are still not entirely understood. We hypothesized that gene expression during the prophase I arrest is primarily epigenetically regulated. Here w...
HydroxyprolineO-arabinosyltransferase mutants oppositely alter tip growth inAra...
MacAlister, Cora A.; Ortiz-Ramírez, Carlos; Becker, Jörg D.; Feijó, José A.; Lippman, Zachary B.Hydroxyproline O-arabinosyltransferases (HPATs) are members of a small, deeply conserved family of plant-specific glycosyltransferases that add arabinose sugars to diverse proteins including cell wall-associated extensins and small signaling peptides. Recent genetic studies in flowering plants suggest that different HPAT homologs have been co-opted to function in diverse species-specific developmental contexts....
Transcriptomic and metabolomic profiling of ionic liquid stimuli unveils enhanc...
Alves, Paula C.; Hartmann, Diego O.; Núñez, Oscar; Martins, Isabel; Gomes, Teresa L.; Garcia, Helga; Galceran, Maria Teresa; Hampson, RichardThe inherent potential of filamentous fungi, especially of Ascomycota, for producing diverse bioactive metabolites remains largely silent under standard laboratory culture conditions. Innumerable strategies have been described to trigger their production, one of the simplest being manipulation of the growth media composition. Supplementing media with ionic liquids surprisingly enhanced the diversity of extracel...
Intercellular communication inArabidopsis thalianapollen discovered viaAHG3tran...
Jiang, Hua; Yi, Jun; Boavida, Leonor C.; Chen, Yuan; Becker, Jörg D.; Köhler, Claudia; McCormick, SheilaAn Arabidopsis pollen grain (male gametophyte) consists of three cells: the vegetative cell, which forms the pollen tube, and two sperm cells enclosed within the vegetative cell. It is still unclear if there is intercellular communication between the vegetative cell and the sperm cells. Here we show that ABA-hypersensitive germination3 (AHG3), encoding a protein phosphatase, is specifically transcribed in the v...
Genome-Wide Analysis of PAPS1-Dependent Polyadenylation Identifies Novel Roles ...
Kappel, Christian; Trost, Gerda; Czesnick, Hjördis; Ramming, Anna; Kolbe, Benjamin; Vi, Son Lang; Bispo, Cláudia; Becker, Jörg D.; de Moor, CorneliaThe poly(A) tail at 3' ends of eukaryotic mRNAs promotes their nuclear export, stability and translational efficiency, and changes in its length can strongly impact gene expression. The Arabidopsis thaliana genome encodes three canonical nuclear poly(A) polymerases, PAPS1, PAPS2 and PAPS4. As shown by their different mutant phenotypes, these three isoforms are functionally specialized, with PAPS1 modifying orga...
V-ATPase Proton Pumping Activity Is Required for Adult Zebrafish Appendage Rege...
Monteiro, Joana; Aires, Rita; Becker, Jörg D.; Jacinto, António; Certal, Ana C.; Rodríguez-León, JoaquínThe activity of ion channels and transporters generates ion-specific fluxes that encode electrical and/or chemical signals with biological significance. Even though it is long known that some of those signals are crucial for regeneration, only in recent years the corresponding molecular sources started to be identified using mainly invertebrate or larval vertebrate models. We used adult zebrafish caudal fin as ...