8 documents found, page 1 of 1

Generation and characterization of induced pluripotent stem cell line (IBBISTi0...

Angelman Syndrome is a rare neurodevelopmental disorder caused by several (epi)genetic alterations. The patients present strong neurological impairment due to the absence of a functional maternal UBE3A gene in neurons. Here, we generated and characterized a new induced pluripotent stem cell (iPSC) line from a female child with Angelman syndrome harbouring a class II deletion. iPSCs were reprogrammed from fibrob...

Might synthetic cannabinoids influence neural differentiation?

Transcriptome profiling of human pluripotent stem cell‐derived cerebellar organ...

Human-induced pluripotent stem cells (iPSCs) have great potential for disease modeling. However, generating iPSC-derived models to study brain diseases remains a challenge. In particular, the ability to recapitulate cerebellar development in vitro is still limited. We presented a reproducible and scalable production of cerebellar organoids by using the novel single-use Vertical-Wheel bioreactors, in which funct...

hiPSC-based model of prenatal exposure to cannabinoids: effect on neuronal diff...

Phytocannabinoids are psychotropic substances ofcannabis with the ability to bind endocannabinoid (eCB) receptors that regulate synaptic activity in the central nervous system (CNS). Synthetic cannabinoids (SCs) are synthetic analogs of Δ9-tetrahydrocannabinol (Δ9-THC), the psychotropic compound of cannabis, acting as agonists of eCB receptor CB1. SC is an easily available and popular alternative to cannabis, a...

Modeling Rett syndrome with human patient-specific forebrain organoids

Engineering brain organoids from human induced pluripotent stem cells (hiPSCs) is a powerful tool for modeling brain development and neurological disorders. Rett syndrome (RTT), a rare neurodevelopmental disorder, can greatly benefit from this technology, since it affects multiple neuronal subtypes in forebrain sub-regions. We have established dorsal and ventral forebrain organoids from control and RTT patient-...

In vitro and in silico evaluations of resin-based dental restorative material t...

Objectives: This study aims to evaluate the cytocompatibility of three provisional restoration materials and predict neurotoxic potential of their monomers. These materials are Tab 2000® (methyl methacrylate based), ProTemp 4™ (bis-acrylic based) and Structur 3® (urethane dimethacrylate based). Materials and methods: Resin samples were incubated in a cell culture medium and the cytotoxic effects of these extrac...

Quantification of cell cycle kinetics by EdU (5-ethynyl-2′-deoxyuridine)-couple...

We propose a novel single-deoxynucleoside-based assay that is easy to perform and provides accurate values for the absolute length (in units of time) of each of the cell cycle stages (G1, S and G2/M). This flow-cytometric assay takes advantage of the excellent stoichiometric properties of azide-fluorochrome detection of DNA substituted with 5-ethynyl-2'-deoxyuridine (EdU). We show that by pulsing cells with EdU...

Bilirubin as a Determinant for Altered Neurogenesis, Neuritogenesis, and Synapt...

Elevated levels of serum unconjugated bilirubin (UCB) in the first weeks of life may lead to long-term neurologic impairment. We previously reported that an early exposure of developing neurons to UCB, in conditions mimicking moderate to severe neonatal j. - Fundacao para a Ciencia e a Tecnologia (FCT) ; FEDER [POCI/SAU-MMO/55955/2004, PTDC/SAU-NEU/64385/2006, SFRH/BPD/26390/2006, SFRH/BPD/26381/2006]; EU FP6 I...