5 documents found, page 1 of 1
Enrichment of hepatic glycogen and plasma glucose from H₂18 O informs gluconeog...
Coelho, Margarida; Mahar, Rohit; Belew, Getachew D.; Torres, Alejandra; Barosa, Cristina; Cabral, Fernando; Viegas, Ivan; Gastaldelli, AmaliaDeuterated water (2 H2 O) is a widely used tracer of carbohydrate biosynthesis in both preclinical and clinical settings, but the significant kinetic isotope effects (KIE) of 2 H can distort metabolic information and mediate toxicity. 18 O-water (H2 18 O) has no significant KIE and is incorporated into specific carbohydrate oxygens via well-defined mechanisms, but to date it has not been evaluated in any animal...
The Gut Microbiome Responds Progressively to Fat and/or Sugar-Rich Diets and Is...
Pessoa, João; Belew, Getachew D.; Barroso, Cristina; Egas, Conceição; Jones, John G.Describing diet-related effects on the gut microbiome is essential for understanding its interactions with fat and/or sugar-rich diets to promote obesity-related metabolic diseases. Here, we sequenced the V3-V4 hypervariable region of the bacterial 16S rRNA gene to study the composition and dynamics of the gut microbiome of adult mice fed diets rich in fat and/or sugar, at 9 and 18 weeks of diet. Under high-fat...
Estimating pentose phosphate pathway activity from the analysis of hepatic glyc...
Belew, Getachew D.; Di Nunzio, Giada; Tavares, Ludgero; Silva, Joao G.; Torres, Alejandra N.; Jones, John GriffithThe pentose phosphate pathway (PPP) is an important component of hepatic intermediary metabolism. Jin et al developed an elegant 13 C-NMR method for measuring hepatic PPP flux by quantifying the distribution of glucose 13 C-isotopomers formed from [U-13 C]glycerol. We demonstrate that this approach can be extended to exogenous [U-13 C]fructose and [U-13 C]glucose precursors by 13 C-NMR analysis of glycogen.; Re...
Determining the contribution of a high-fructose corn syrup formulation to hepat...
DiNunzio, Giada; Belew, Getachew D.; Torres, Alejandra N.; Silva, Joao Gabriel; Silva, Luís P.; Barosa, Cristina; Tavares, Ludgero; Jones, John G.Excessive sugar intake including high-fructose corn syrup (HFCS) is implicated in the rise of obesity, insulin resistance and non-alcoholic fatty liver disease. Liver glycogen synthesis is influenced by both fructose and insulin signaling. Therefore, the effect of HFCS on hepatic glycogenesis was evaluated in mice feeding ad-libitum. Using deuterated water: the fraction of glycogen derived from triose-P sources...
A Mitochondrial Approach to Cardiovascular Risk and Disease
Veloso, Caroline D.; Belew, Getachew D.; Ferreira, Luciana L.; Grilo, Luís F.; Jones, John Griffith; Portincasa, Piero; Sardão, Vilma A.Cardiovascular diseases (CVDs) are a leading risk factor for mortality worldwide and the number of CVDs victims is predicted to rise through 2030. While several external parameters (genetic, behavioral, environmental and physiological) contribute to cardiovascular morbidity and mortality; intrinsic metabolic and functional determinants such as insulin resistance, hyperglycemia, inflammation, high blood pressure...