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Correction
Almeida, Micael; Inácio, José M.; Vital, Carlos M.; Rodrigues, Madalena R.; Araújo, Beatriz C.; Belo, José A.After the publication of the review article [1], one of the referenced manuscripts was found to have been retracted. To enhance the quality of the manuscript, we decided to replace the previous reference number 157 [2] from the original manuscript with the new reference [3] at the same position in the reference list. Original reference number 157: Zhang, D.; Ning, J.; Ramprasath, T.; Yu, C.; Zheng, X.; Song, P....
Cell Reprogramming, Transdifferentiation, and Dedifferentiation Approaches for ...
Almeida, Micael; Inacio, Jose M; Vital, Flávia M R; Rodrigues, Madalena; Araújo, Beatriz C.; Belo, José A.Funding Information: This work was funded by FUNDA\u00C7\u00C3O PARA A CI\u00CANCIA E A TECNOLOGIA, grant number PTDC/BIMMED/3363/2014 to J.A.B., a predoctoral fellowship to M.A. (grant number 06890/2021/BD). This work was supported by the Research Unit UID/04462: iNOVA4Health\u2014Programa de Medicina Translacional and by the Associated Laboratory LS4FUTURE (LA/P/0087/2020), all financially supported by Funda\...
Myocardial RNA Sequencing Reveals New Potential Therapeutic Targets in Heart Fa...
Inácio, José M.; Cristo, Fernando; Pinheiro, Miguel; Vasques-Nóvoa, Francisco; Saraiva, Francisca; Nunes, Mafalda M.; Rosas, Graça; Reis, AndreiaFunding Information: This work was supported by the Fundação para a Ciência e a Tecnologia (NETDIAMOND-SAICTPAC/0047/2015), Scientific Employment Stimulus to J.M.I. (Norma Transitória 8189/2018), post-doctoral fellowship to F.C. (DAI/2019/08/SAICTPAC/0047/2015), iNOVA4Health-UID/Multi/04462/2020 and LS4FUTURE Associated Laboratory (LA/P/0087/2020). Genome Medicine Lab is funded by FCT and COMPETE2020 (iBiMED: U...
A novel biallelic loss-of-function variant in DAND5 causes heterotaxy syndrome
Ganapathi, Mythily; Buchovecky, Christie M.; Cristo, Fernando; Ahimaz, Priyanka; Ruzal-Shapiro, Carrie; Wou, Karen; Inácio, José M.; Iglesias, AlejandroFunding J.A.B. acknowledges funding from iNOVA4Health-UID/Multi/04462/2013, a program financially supported by Fundação para a Ciência e Tecnologia/Ministério da Educação e Ciência through national funds and cofunded by Fundo Europeu de Desenvolvimento Regional (FEDER) under the PT2020 Partnership Agreement.; The majority of heterotaxy cases do not obtain a molecular diagnosis, although pathogenic variants in m...
CCBE1 Is Essential for Epicardial Function during Myocardium Development
Bonet, Fernando; Añez, Sabrina Brito; Inácio, José Manuel; Futschik, Matthias E.; Belo, José A.Funding: This work was supported by Fundação para a Ciência e a Tecnologia (FCT) grants (PTDC/MEC-CAR/29590/2017) to JA Belo and by the Scientific Employment Stimulus to JMI (Norma Transitória 8189/2018, FCT), and iNOVA4Health—UIDB/Multi/04462/2013, a program Int. J. Mol. Sci. 2022, 23, 12642 14 of 17 financially supported by Fundação para a Ciência e Tecnologia/Ministério da Educação e Ciência, through nationa...
Generation and characterization of a human iPS cell line from a patient-related...
Pars, Selin; Cristo, Fernando; Inácio, José M.; Rosas, Graça; Carreira, Isabel Marques; Melo, Joana Barbosa; Mendes, Patrícia; Martins, Duarte SaraivaA DAND5-control human iPSC line was generated from the urinary cells of a phenotypically normal donor. Exfoliated renal epithelial (RE) cells were collected and reprogrammed into iPSCs using Sendai virus reprogramming system. The pluripotency, in vitro differentiation potential, karyotype stability, and the transgene-free status of generated iPSC line were analyzed and confirmed. This cell line can be exploited...
Loss of Ccbe1 affects cardiac-specification and cardiomyocyte differentiation i...
Bover, Oriol; Justo, Tiago; Pereira, Paulo N. G.; Facucho-Oliveira, João; Inácio, José M.; Ramalho, José S.; Domian, Ibrahim J.; Belo, José A.Understanding the molecular pathways regulating cardiogenesis is crucial for the early diagnosis of heart diseases and improvement of cardiovascular disease. During normal mammalian cardiac development, collagen and calcium-binding EGF domain-1 (Ccbe1) is expressed in the first and second heart field progenitors as well as in the proepicardium, but its role in early cardiac commitment remains unknown. Here we d...
Generation of human iPSC line from a patient with laterality defects and associ...
Cristo, Fernando; Inácio, José M; Rosas, Graça; Carreira, Isabel Marques; Melo, Joana Barbosa; Almeida, Luís Pereira de; Mendes, PatríciaA human iPSC line was generated from exfoliated renal epithelial (ERE) cells of a patient affected with Congenital Heart Disease (CHD) and Laterality Defects carrying tshe variant p.R152H in the DAND5 gene. The transgene-free iPSCs were generated with the human OSKM transcription factor using the Sendai-virus reprogramming system. The established iPSC line had the specific heterozygous alteration, a stable kary...
Matrix Gla Protein expression pattern in the early avian embryo
Correia, Elizabeth; Conceição, Natércia; Cancela, Leonor; Belo, José A.MGP (Matrix Gla Protein) is an extracellular matrix vitamin K dependent protein previously identified as a physiological inhibitor of calcification and shown to be well conserved among vertebrates during evolution. MGP is involved in other mechanisms such as TGF-beta and BMP activity, and a proposed modulator of cell-matrix interactions. MGP is expressed early in vertebrate development although its role has not...
Novel triblock co-polymer nanofibre system as an alternative support for embryo...
Perestrelo, Ana Rubina; Mouffouk, Fouzi; Costa, Ana M. Rosa da; Belo, José A.Conventionally, embryonic stem cells (ESCs) are cultured on gelatin or over a mitotically inactivated monolayer of mouse embryonic fibroblasts (MEFsi). Considering the lack of versatile, non-animal-derived and inexpensive materials for that purpose, we aimed to find a biomaterial able to support ESC growth in a pluripotent state that avoids the need for laborious and time-consuming MEFsi culture in parallel wit...