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Sustainable Strategy to Boost Legumes Growth under Salinity and Drought Stress ...

Ben Gaied, Roukaya; Brígido, Clarisse; Sbissi, I.; Tarhouni, M.

The escalating risks of drought and salinization due to climate change and anthropogenic activities are a major global concern. Rhizobium–legume (herb or tree) symbiosis is proposed as an ideal solution for improving soil fertility and rehabilitating arid lands, representing a crucial direction for future research. Consequently, several studies have focused on enhancing legume tolerance to drought and salinity ...


Genomic Insights into a Desert-Adapted Sinorhizobium Strain from the Tunisian S...

Ben Gaied, Roukaya; Sbissi, I.; Kaur, S.; diCenzo, G. C; Brígido, Clarisse

Nitrogen fixation by rhizobia in symbiosis with legumes is essential for nitrogen cycling and sustainable agriculture and plays a critical role in the survival of native leguminous plants in the arid and Saharan environments of Tunisia. The Sinorhizobium meliloti strain IRAMC:0087, initially isolated from the root nodules of the Saharan shrub Calobota saharae in southern Tunisia, can establish nodulation with V...


Enhancing Pisum sativum growth and symbiosis under heat stress: the synergistic...

Ben Gaied, Roukaya; Sbissi, Imed; Tarhouni, Mohamed; Brígido, Clarisse

The 1-aminocyclopropane-1-carboxylate (ACC) deaminase is a crucial bacterial trait, yet it is not widely distributed among rhizobia. Hence, employing a co-inoculation approach that combines selected plant growth-promoting bacteria with compatible rhizobial strains, especially those lacking ACC deaminase, presents a practical solution to alleviate the negative effects of diverse abiotic stresses on legume nodula...


Genomic analysis of a Sinorhizobium strain isolated from the Tunisian desert

Ben Gaied, Roukaya; Sbissi, Imed; Kaur, Sabhjeet; diCenzo, George C.; Tarhouni, Mohamed; Brígido, Clarisse

The N2-fixation by rhizobia in symbiosis with legumes is critical to global nitrogen cycling and sustainable agriculture[1] and for survival and prevalence of endemic spontaneous legume’s in Arid and Saharan regions of Tunisia. The strain Sinorhizobium meliloti IRAMC:0087 was originally isolated from root nodules of a Saharan shrub Calobota saharae growing in the Saharan regions of Southern Tunisia. IRAMC:0087 ...


Genomic analysis of a Sinorhizobium strain isolated from the Tunisian desert

Ben Gaied, Roukaya; Sbissi, Imed; Kaur, Sabhjeet; diCenzo, George C.; Tarhouni, Mohamed; Brígido, Clarisse

The N2-fixation by rhizobia in symbiosis with legumes is critical to global nitrogen cycling and sustainable agriculture[1] and for survival and prevalence of endemic spontaneous legume’s in Arid and Saharan regions of Tunisia. The strain Sinorhizobium meliloti IRAMC:0087 was originally isolated from root nodules of a Saharan shrub Calobota saharae growing in the Saharan regions of Southern Tunisia. IRAMC:0087 ...


From arid wild legume shrubs to food legume: Application of non-rhizobial bacte...

Ben Gaied, Roukaya; Sbissi, Imed; Tarhouni, Mohamed; Brígido, Clarisse

Chickpea (Cicer arietinum L.) is an important grain legume with a high nutritional value. Being the third-largest food legume produced worldwide, chickpea is mainly cultivated in developing countries where crop farming suffers the impacts of various biotic and abiotic constraints such as heat, salinity, and drought. In such vulnerable areas, the increase of soil salinity results in an annual loss of 8-10% of ch...


Bacterial Endophytes from Legumes Native to Arid Environments Are Promising Too...

Ben Gaied, Roukaya; Sbissi, Imed; Tarhouni, Mohamed; Brígido, Clarisse

Symbiotic nitrogen fixation is a major contributor of N in agricultural ecosystems, but the establishment of legume–rhizobium symbiosis is highly affected by soil salinity. Our interest is focused on the use of non-rhizobial endophytes to assist the symbiosis between chickpea and its microsymbiont under salinity to avoid loss of production and fertility. Our aims were (1) to investigate the impact of salinity o...


Genomic analysis of Sinorhizobium meliloti IRAMC:0087 an halotolerant rhizobium...

Ben Gaied, Roukaya; Sbissi, Imed; Brígido, Clarisse; Tarhouni, Mohamed

Arid and Saharan regions of Tunisia have great interest due to their endemic spontaneous legume’s diversity. The strain IRAMC:0087 was isolated from root nodules of a Saharan shrub Genista saharae growing in Southern Tunisia. Phenotypic characterization of this strain has revealed tolerance to high salinity levels, drought and high temperatures. To investigate the basis of this, we sequenced its complete genome...


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