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Platelet-rich plasma in the treatment of induced tendinopathy in horses: histol...
Maia, Leandro; Souza, Maria Verônica de; Ribeiro Júnior, José Ivo; Oliveira, Aécio Carlos de; Alves, Geraldo Eleno Silveira; Benjamin, Laércio dos AnjosThis study was carried out to evaluate the effect of platelet-rich plasma (PRP) on the treatment of tendinopathy induced in the superficial digital flexor tendon (TFDS) of horses, by using histologic evaluation. Six healthy crossbred geldings aged 8 to 15 years (12 ± 3) were used. The TFDS tendinopathy was provoked in both forelimbs, by intratendinous administration of 2.5 mg collagenase (2.5 mg/mL), and this p...
Bacupari peel extracts (Garcinia brasiliensis) reduces the biometry, lipogenesi...
Moreira, Maria Eliza de Castro; Araújo, Fernanda de Oliveira; Sousa, Andressa Rodrigues de; Toledo, Renata Celi Lopes; Benjamin, Laércio dos AnjosThe aim was to evaluate the effect of the ethanol extract of bacupari peel (EEB) on biometric measurements, hepatic lipogenesis and progression of non-alcoholic fatty liver disease (NAFLD) in obese Wistar rats. Chemical analysis of the bacupari peel extract identified 7-epiclusianone as the major constituent (140.02 mg/g) followed by morelloflavone (35.86 mg/g). Animals treated with high fat diet plus EEB (BHFD...
The seminiferous epithelium cycle and daily spermatic production in the adult m...
Bitencourt, Viviane Lewicki; Paula, Tarcı́zio Antônio Rego de; Matta, Sérgio Luis Pinto da; Fonseca, Cláudio César; Benjamin, Laércio dos AnjosThe duration of the seminiferous epithelium cycle was estimated in adult maned wolves (Chrysocyon brachyurus, Illiger, 1811), by applying intratesticular injections with tritiated thymidine. The total duration of the seminiferous epithelium cycle in this species was calculated in 8.99 days. So, taking into account that approximately 4.5 cycles of the seminiferous epithelium are necessary for the whole spermatog...
The human follicle stimulating hormone (hFSH) keeps the normal ultrastructure o...
Costa, Sanely Lourenço da; Costa, Eduardo Paulino da; Pereira, Emílio César Martins; Benjamin, Laércio dos Anjos; Verde, Isabel Bezerra LimaO objetivo desse estudo foi investigar os efeitos do FSH humano (hFSH) no cultivo in vitro de folículos pré-antrais caprinos. Fragmentos do córtex ovariano de caprinos foram cultivados em ?-MEM+ suplementado com 0, 10, ou 50 ng/mL de hFSH por um ou sete dias. Pequenos fragmentos de tecido ovariano cultivado e não cultivados, foram processados por histologia clássica e microscopia eletrônica de transmissão. Os t...
Infecção experimental em bovinos: cinética da produção de imunoglobulinas IgM e...
Guimarães-Peixoto, Rafaella Paola Meneguete dos; Pinto, Paulo Sérgio de Arruda; Benjamin, Laércio dos Anjos; Oliveira, Leandro Licursi de; Marcon, LucasA cisticercose bovina é uma zoonose que acomete humanos em sua forma adulta (teníase) e em sua forma larvar encontra-se inserida na musculatura de bovinos infectados (cisticercos). Ainda não está totalmente esclarecido como ocorre a resposta imune animal frente a infecção por cisticercos, sendo necessário sua total compreensão para aprimoramento de testes diagnósticos e prevenção da doença. Este trabalho teve o...
Influence of the preservation period in silica-gel on the predatory activity of...
Braga, Fabio Ribeiro; Araújo, Jackson Victor; Araujo, Juliana Milani; Tavela, Alexandre de Oliveira; Ferreira, Sebastião RodrigoThe continued maintenance of nematophagous fungi predatory activity under laboratory conditions is one of the basic requirements for a successful biological control. The purpose of this study was to evaluate the influence of time on the preservation of the fungus Duddingtonia flagrans (AC001 and CG722) stored in silica-gel for 7 years and their subsequent predatory activity on cyathostomin L3 larvae in 2% water...
Immunohistochemical, morphological and histometrical analyses of follicular dev...
Marcon, Lucas; Thomé, Ralph Gruppi; Mounteer, Ann Honor; Bazzoli, Nilo; Rizzo, Elizete; Benjamin, Laércio dos AnjosThiodan® is an organochlorine insecticide used in agriculture that can reach aquatic ecosystems where it can affect fish reproduction. This research aimed to evaluate follicular development and the expression of integrin β1, collagen type IV and caspase 3 by morphological, histometrical and immunohistochemical analyses of Astyanax bimaculatus exposed to Thiodan®. Treatments included three sublethal concentratio...
Coadministration of sodium alginate pellets containing the fungi Duddingtonia f...
Tavela, Alexandre de Oliveira; Araújo, Jackson Victor de; Braga, Fábio Ribeiro; Silveira, Wendeo Ferreira da; Silva, Vinicius Herold Dornelas eThe predatory nematophagous fungi have been used as an alternative control of gastrointestinal nematodes of domestic animals in natural and laboratory conditions. However, it is unclear if the association of some of these species could bring some kind of advantage, from a biological standpoint. In this context, this study consisted of two tests in vitro: in assay A, the assessment of the viability of the associ...
Contribuições para a adoção de uma cultura de divulgação, valorização e de resp...
Zuanon, Átima Clemente Alves; Benjamin, Laércio dos Anjos; Fonseca, Cláudio CésarA busca por mecanismos que promovam melhorias das atividades que são atribuídas aos Comitês ou Comissões de Ética no Uso de Animais, nas atividades didático-científicas, caracteriza uma interface adicional e imprescindível no debate emergente nas instituições de ensino superior. O ideal objetivado é o de descentralizar, conhecer e, sobretudo, disponibilizar informações técnicas e legais sobre o assunto, bem com...
Pathological and histometric analysis of the gills of female Hyphessobrycon equ...
Lopes, Diego Senra; Leandro, Mila Vasques; Goulart, Amara Manarino Andrade; Benjamin, Laércio dos Anjos; Mounteer, Ann Honor; Marcon, LucasFemale individuals of Hyphessobrycon eques were exposed to Diflubenzuron (Dimilin®) in order to determine whether exposure to sublethal levels of this insecticide causes changes in gill morphology. Fish were exposed to 0.01, 0.1 and 1.0 mg L−1 for 96 h and 17 days and then submitted to pathological and histometric evaluation. Pathological lesions, such as hyperplasia, lamellar fusion, vascular congestion, secon...