3 documents found, page 1 of 1
Supported ionic liquids to purify microbial L-Asparaginase
Almeida, Mafalda R.; Nunes, João C. F.; Pereira, Matheus M.; Bento, Heitor B. S.; Pedrolli, Danielle B.; Santos-Ebinuma, Valéria C.; Neves, Márcia C.L-Asparaginase (ASNase) is a versatile enzyme that converts L-asparagine into ammonia and aspartic acid. This enzyme has applications in the food industry and health sector. However, high purity ASNase is required, resulting in high production costs. Therefore, in this work, two supported ionic liquids (SILs), specifically silica modified with dimethylbutylpropylammonium chloride ([Si][N3114]Cl) or triethylprop...
L-asparaginase production review: bioprocess design and biochemical characteris...
Castro, Daniel; Marques, Ana Sofia C.; Almeida, Mafalda R.; Paiva, Gabriela B. de; Bento, Heitor B. S.; Pedrolli, Danielle B.; Freire, Mara G.In the past decades, production of biopharmaceuticals has gained high interest due to its high sensitivity, specificity and lower risk of negative effects to patients. Biopharmaceuticals are mostly therapeutic recombinant proteins produced through biotechnological processes. In this context, L-Asparaginase (L-Asparagine amidohydrolase, L-ASNase (E.C. 3.5.1.1)) is a therapeutic enzyme that has been abundantly st...
Production and characterization of recombinant Aliivibrio fischeri L-Asparagina...
Bento, Heitor B. S.; Paiva, Gabriela B.; Pedrolli, Danielle B.; Tavares, Ana P. M.; Santos-Ebinuma, ValériaL-Asparaginase has been successfully applied in the treatment of lymphoid malignancies. Some limitations in the use of the commercial preparations of this drug include several side effects that may be correlated to L-Glutaminase activity, as immunosuppressive effects (Castro et al., 2021). The objective of this study was to evaluate the characteristics of a novel engineered Aliivibrio fischeri L-asparaginase ty...