10 documents found, page 1 of 1
EFEITO IN VITRO E IN VIVO DE EXTRATOS DE Eugenia uniflora EM NEMATÓDEOS GASTRIN...
Castro,Luciana Laitano Dias de; Sprenger,Lew Kan; Madrid,Isabel Martins; Oliveira,Fernando Caetano de; Oliveira,Plíno Aguiar deResumo Este estudo avaliou a ação in vitro e in vivo de extratos de Eugenia uniflora em nematódeos gastrintestinais de ovinos. No teste de inibição da eclodibilidade dos ovos, extratos aquosos e hidroalcoólicos foram testados nas concentrações de 40 a 1,25 mg/mL. O efeito citotóxico foi mensurado através do ensaio de MTT em células VERO nas concentrações de 4000 a 1,95 µg/mL. A composição química foi analisada ...
Galectins and collectinis expression are increased in Haemonchus contortus-infe...
Souza,Bárbara Maria Paraná da Silva; Lambert,Sabrina Mota; Nishi,Sandra Mayumi; Benavides,Magda Vieira; Berne,Maria Elisabeth AiresGalectins and collectins are proteins classified in the lectin family that have the ability to recognize molecular patterns associated with pathogens. Studies on cattle have demonstrated high expression of these proteins during infection with gastrointestinal nematodes. The aim of this study was to investigate whether the level of Haemonchus contortus infection would alter the expression of galectins (Gal11 and...
Use of ELISA based on NcSRS2 of Neospora caninumexpressed in Pichia pastoris fo...
Pinheiro,Amanda Fernandes; Borsuk,Sibele; Berne,Maria Elisabeth Aires; Pinto,Luciano da Silva; Andreotti,Renato; Roos,Talita; Roloff,Barbara CoutoNeosporosis is a disease caused by the protozoon Neospora caninum that leads to significant economic losses in many countries. In the present study, we report on use of the recombinant protein NcSRS2 of N. caninum expressed in Pichia pastoris in an indirect immunoenzymatic assay (ELISA) for diagnosing neosporosis infection in sheep and dogs. We observed that the ELISA test yielded specificity of 94.5% and sensi...
Determination of IgG avidity in BALB/c mice experimentally infected with Toxoca...
Schoenardie,Elizandra Roselaine; Scaini,Carlos James; Avila,Luciana Farias da Costa de; Sperotto,Rita Leal; Borsuk,Sibele; Felicetti,Cristine Dias PiresToxocariasis is a zoonotic disease in that IgM titers can remain high for long periods making difficult to determine the stage of the disease. The aim of this study is to investigate the applicability of indirect ELISA, associated with urea, to discriminate between the acute and chronic toxocariasis. IgG avidity was evaluated in 25 BALB/c mice experimentally infected with 1000 Toxocara canis eggs. Blood samples...
Conventional serological performance in diagnosis of Chagas' disease in souther...
Araújo,Anelise Bergmann; Berne,Maria Elisabeth AiresResults of Chagas' disease diagnosis show disagreement. The aim of this study was to compare commercial tests for Chagas' disease serodiagnosis in southern Brazil. A total of 161 samples were evaluated. Three enzyme-linked immunosorbent assays, one indirect hemagglutination and one indirect immunofluorescence were assessed. Trypomastigote excreted-secreted antigen-blot was a confirmatory method. From 161 sample...
Frequency of geohelminths in public squares in Pelotas, RS, Brazil
Moura,Micaele Quintana de; Jeske,Sabrina; Vieira,Juliana Nunes; Corrêa,Tiago Gallina; Berne,Maria Elisabeth Aires; Villela,Marcos MarreiroThe frequency of parasitic contamination of public areas in the municipality of Pelotas, state of Rio Grande do Sul, Brazil, was studied between June 2010 and May 2011, when soil samples were collected from eight city squares. Out of 400 samples submitted to centrifugal floatation technique in solution of sodium dichromate with density of 1.35, 176 (44%) proved positive for at least one parasite; 29 (16.5%) sam...
Vertical transmission of Toxocara canis in successive generations of mice
Schoenardie,Elizandra Roselaine; Scaini,Carlos James; Pepe,Michele Soares; Borsuk,Sibele; Avila,Luciana Farias da Costa de; Villela,MarcosVisceral toxocariasis is a neglected zoonosis caused by Toxocara canis larvae in unusual hosts. In dogs, the definitive host, the infection occurs mainly through transplacental and transcolostral transmission. Studies on experimental models have shown that vertical transmission may result from acute infections. Considering that toxocariasis is characterized as a chronic infection, with possible reactivation of ...
Origanum vulgare (Lamiaceae) ovicidal potential on castrointestinal nematodes o...
Castro,Luciana Laitano Dias de; Madrid,Isabel Martins; Aguiar,Cíntia Lidiane Guidotti; Castro,Leonardo Mortagua de; Cleff,Marlete BrumDue to anthelmintic resistance in nematodes, several research studies have been developed seeking control alternatives to these parasites. This study evaluated the in vitro action of Origanum vulgare on gastrointestinal nematode eggs of cattle. In order to evaluate the ability to inhibit egg hatch, different dried leaves extracts of this plant were tested, such as dye, hydroalcoholic and aqueous extracts at con...
Presence of eggs of Toxocara spp. and hookworms in a student environment in Rio...
Gallina,Tiago; Silva,Maria Antonieta Machado Pereira da; Castro,Luciana Laitano Dias de; Wendt,Emília Welter; Villela,Marcos MarreiroEnvironmental contamination by parasite forms was studied on a university campus in the municipality of Pelotas, Rio Grande do Sul. Soil samples were analyzed using the modified Caldwell & Caldwell technique to identify parasite forms. Nematode eggs were detected in 62% of the samples. Among the parasites detected, eggs of Toxocara spp. and Ancylostomatidae were the most prevalent parasites in the studied a...
Prevalência da doença de Chagas em gestantes da região sul do Rio Grande do Sul
Araújo,Anelise Bergmann; Castagno,Victor Delpizzo; Gallina,Tiago; Berne,Maria Elisabeth AiresAnticorpos antiTrypanosoma cruzi no cordão umbilical de 351 parturientes da Cidade de Pelotas, RS foram pesquisados a fim de investigar a prevalência da doença de Chagas em gestantes. Um (0,3%) caso foi identificado, não sendo detectada transmissão congênita. Salienta-se a importância da investigação da doença de Chagas em gestantes de zonas endêmicas ou provenientes destas.