8 documents found, page 1 of 1
Endocan as a marker of endotheliitis in COVID-19 patients: modulation by veno-v...
Reina-Couto, M; Alves, D; Silva-Pereira, C; Pereira-Terra, P; Martins, S; Bessa, J; Teixeira-Santos, L; Pinho, D; Morato, M; Dias, CC; Sarmento, A"Background and aims: Endocan has been scarcely explored in COVID-19, especially regarding its modulation by veno-venous extracorporeal membrane oxygenation (VV-ECMO), hypertension or previous renin-angiotensin-aldosterone system (RAAS) inhibitors treatment. We compared endocan and other endotheliitis markers in hospitalized COVID-19 patients and assessed their modulation by VV-ECMO, hypertension and previous R...
Proinflammatory and endothelial activation profiles in hospitalized COVID-19 pa...
Silva-Pereira, PDC; Reina-Couto, M; Alves, D; Pereira-Terra, P; Martins, S; Bessa, J; Teixeira-Santos, L; Pinho, D; Morato, M; Sarmento, A; Tavares, Mn/a
Foxm1 modulates cell non-autonomous response in zebrafish skeletal muscle homeo...
Ferreira, FJ; Carvalho, L; Logarinho, E; Bessa, JFoxm1 is a master regulator of the cell cycle, contributing to cell proliferation. Recent data have shown that this transcription factor also modulates gene networks associated with other cellular mechanisms, suggesting non-proliferative functions that remain largely unexplored. In this study, we used CRISPR/Cas9 to disrupt foxm1 in the zebrafish terminally differentiated fast-twitching muscle cells. foxm1 geno...
FOXM1 repression increases mitotic death upon antimitotic chemotherapy through ...
Vaz, S; Ferreira, FJ; Macedo, JC; Leor, G; Ben-David, U; Bessa, J; Logarinho, EInhibition of spindle microtubule (MT) dynamics has been effectively used in cancer treatment. Although the mechanisms by which MT poisons elicit mitotic arrest are fairly understood, efforts are still needed towards elucidating how cancer cells respond to antimitotic drugs owing to cytotoxicity and resistance side effects. Here, we identified the critical G2/M transcription factor Forkhead box M1 (FOXM1) as a ...
Antisense transcription across nucleotide repeat expansions in neurodegenerativ...
Castro, AF; Loureiro, JR; Bessa, J; Silveira, IUnstable repeat expansions and insertions cause more than 30 neurodegenerative and neuromuscular diseases. Remarkably, bidirectional transcription of repeat expansions has been identified in at least 14 of these diseases. More remarkably, a growing number of studies has been showing that both sense and antisense repeat RNAs are able to dysregulate important cellular pathways, contributing together to the observ...
A Conserved Notochord Enhancer Controls Pancreas Development in Vertebrates
Amorim, JP; Gali-Macedo, A; Marcelino, H; Bordeira-Carriço, R; Naranjo, S; Rivero-Gil, S; Teixeira, J; Galhardo, M; Marques, JF; Bessa, JThe notochord is an evolutionary novelty in vertebrates that functions as an important signaling center during development. Notochord ablation in chicken has demonstrated that it is crucial for pancreas development; however, the molecular mechanism has not been fully described. Here, we show that in zebrafish, the loss of function of nog2, a Bmp antagonist expressed in the notochord, impairs ß cell differentiat...
CRISPR-Cas9-Mediated Genomic Deletions Protocol in Zebrafish
Amorim, JP; Bordeira-Carriço, R; Gali-Macedo, A; Perrod, C; Bessa, JSince its first application for site-directed mutagenesis, the CRISPR-Cas9 system has revolutionized genome engineering. Here, we present a validated workflow for the generation of targeted genomic deletions in zebrafish, including the design, cloning, and synthesis of single-guide RNAs and Cas9 mRNA, followed by microinjection in zebrafish embryos and subsequent genotype screening for the establishment of a mu...
A pentanucleotide ATTTC repeat insertion in the non-coding region of DAB1, mapp...
Seixas, AI; Loureiro, JR; Costa, C; Ordóñez-Ugalde, A; Marcelino, H; Oliveira, CL; Loureiro, JL; Dhingra, A; Brandão, E; Cruz, VT; Timóteo, AAdvances in human genetics in recent years have largely been driven by next-generation sequencing (NGS); however, the discovery of disease-related gene mutations has been biased toward the exome because the large and very repetitive regions that characterize the non-coding genome remain difficult to reach by that technology. For autosomal-dominant spinocerebellar ataxias (SCAs), 28 genes have been identified, b...