19 documents found, page 1 of 2
Multiparametric in vitro genotoxicity assessment of different variants of amorp...
Brandão, F; Costa, C; Bessa, MJ; Valdiglesias, V; Hellack, B; Haase, A; Fraga, S; Teixeira, JPThe hazard posed to human health by inhaled amorphous silica nanomaterials (aSiO2 NM) remains uncertain. Herein, we assessed the cyto- and genotoxicity of aSiO2 NM variants covering different sizes (7, 15, and 40 nm) and surface modifications (unmodified, phosphonate-, amino- and trimethylsilyl-modified) on rat alveolar epithelial (RLE-6TN) cells. Cytotoxicity was evaluated at 24 h after exposure to the aSiO2 N...
Assessing the in vitro toxicity of airborne (nano)particles to the human respir...
Bessa, MJ; Brandão, F; Rosário, F; Moreira, L; Reis, AT; Valdiglesias, V; Laffon, B; Fraga, S; Teixeira, JPSeveral studies have been conducted to address the potential adverse health risks attributed to exposure to nanoscale materials. While in vivo studies are fundamental for identifying the relationship between dose and occurrence of adverse effects, in vitro model systems provide important information regarding the mechanism(s) of action at the molecular level. With a special focus on exposure to inhaled (nano)pa...
In Vitro Cyto-and Genotoxicity Assessment of Antibacterial Paints with Triclosa...
Querido, MM; Rosário, F; Bessa, MJ; Mendes, F; Teixeira, JC; Teixeira, JP; Pereira, CCSurfaces with antimicrobial properties are gaining notoriety as an efficient method to avoid surface contamination. Self-disinfecting paints are a promising strategy towards cleaner indoor environments by preventing the colonization of walls with microorganisms. However, its widespread use needs an appropriate toxicological safety evaluation due to the potential for biological disturbance associated to its bioc...
Unveiling the Toxicity of Fine and Nano-Sized Airborne Particles Generated from...
Bessa, MJ; Brandão, F; Fokkens, PHB; Leseman, DLAC; Boere, AJF; Cassee, FR; Salmatonidis, A; Viana, M; Monfort, E; Fraga, S; Teixeira, JPHigh-energy industrial processes have been associated with particle release into workplace air that can adversely affect workers' health. The present study assessed the toxicity of incidental fine (PGFP) and nanoparticles (PGNP) emitted from atmospheric plasma (APS) and high-velocity oxy-fuel (HVOF) thermal spraying. Lactate dehydrogenase (LDH) release, 2-(4-nitrophenyl)-2H-5-tetrazolio]-1,3-benzene disulfonate...
In Vitro Toxicity of Industrially Relevant Engineered Nanoparticles in Human Al...
Bessa, MJ; Brandão, F; Fokkens, PHB; Leseman, DLAC; Boere, AJF; Cassee, FR; Salmatonidis, A; Viana, M; Vulpoi, A; Simon, S; Monfort, E; Teixeira, JPDiverse industries have already incorporated within their production processes engineered nanoparticles (ENP), increasing the potential risk of worker inhalation exposure. In vitro models have been widely used to investigate ENP toxicity. Air–liquid interface (ALI) cell cultures have been emerging as a valuable alternative to submerged cultures as they are more representative of the inhalation exposure to airbo...
Toxicity assessment of industrial engineered and airborne process-generated nan...
Bessa, MJ; Brandão, F; Fokkens, P; Cassee, FR; Salmatonidis, A; Viana, M; Vulpoi, A; Simon, S; Monfort, E; Teixeira, JP; Fraga, SThe advanced ceramic technology has been pointed out as a potentially relevant case of occupational exposure to nanoparticles (NP). Not only when nanoscale powders are being used for production, but also in the high-temperature processing of ceramic materials there is also a high potential for NP release into the workplace environment. In vitro toxicity of engineered NP (ENP) [antimony tin oxide (Sb2O3•SnO2; AT...
Characterizing the Chemical Profile of Incidental Ultrafine Particles for Toxic...
Viana, M; Salmatonidis, A; Bezantakos, S; Ribalta, C; Moreno, N; Córdoba, P; Cassee, FR; Boere, J; Fraga, S; Teixeira, JP; Bessa, MJ; Monfort, EIncidental ultrafine particles (UFPs) constitute a key pollutant in industrial workplaces. However, characterizing their chemical properties for exposure and toxicity assessments still remains a challenge. In this work, the performance of an aerosol concentrator (Versatile Aerosol Concentration Enrichment System, VACES) was assessed to simultaneously sample UFPs on filter substrates (for chemical analysis) and ...
Genotoxicity and Gene Expression in the Rat Lung Tissue following Instillation ...
Brandão, F; Costa, C; Bessa, MJ; Dumortier, E; Debacq-Chainiaux, F; Hubaux, R; Salmon, M; Laloy, J; Stan, MS; Hermenean, A; Gharbia, S; Dinischiotu, ASeveral reports on amorphous silica nanomaterial (aSiO2 NM) toxicity have been questioning their safety. Herein, we investigated the in vivo pulmonary toxicity of four variants of aSiO2 NM: SiO2_15_Unmod, SiO2_15_Amino, SiO2_7 and SiO2_40. We focused on alterations in lung DNA and protein integrity, and gene expression following single intratracheal instillation in rats. Additionally, a short-term inhalation st...
Nanoparticle exposure and hazard in the ceramic industry: an overview of potent...
Bessa, MJ; Brandão, F; Viana, M; Gomes, JF; Monfort, E; Cassee, FR; Fraga, S; Teixeira, JPThe ceramic industry is an industrial sector of great impact in the global economy that has been benefiting from advances in materials and processing technologies. Ceramic manufacturing has a strong potential for airborne particle formation and emission, namely of ultrafine particles (UFP) and nanoparticles (NP), meaning that workers of those industries are at risk of potential exposure to these particles. At p...
Genotoxicity of TiO(2) Nanoparticles in Four Different Human Cell Lines (A549, ...
Brandão, F; Fernández-Bertólez, N; Rosário, F; Bessa, MJ; Fraga, S; Pásaro, E; Teixeira, JP; Laffon, B; Valdiglesias, V; Costa, CTitanium dioxide nanoparticles (TiO2 NPs) have a wide variety of applications in many consumer products, including as food additives, increasing the concern about the possible hazards that TiO2 NPs may pose to human health. Although most previous studies have focused on the respiratory system, ingestion must also be considered as an important exposure route. Furthermore, after inhalation or ingestion, TiO2 NPs ...