31 documents found, page 1 of 4
Azadirachta indica A. Juss (neem) phenolic extract inhibits human B-lymphoblast...
Santos, Klebson Silva; Costa, Carla; Bessa, Maria João; Teixeira, João Paulo; Muniz, Ana Veruska Cruz da Silva; Padilha, Francine FerreiraAim: As far as is known, the pharmaceutical effects of neem on human B-lymphoblastoid (TK6) cells have not been studied until now. Hence, the present study aimed to obtain neem phenolic extracts for inhibits the proliferation of TK6 cells and explore some possible underlying mechanisms involved in these effects. Methods: Hexane extract (HE) was obtained in the first step. After that, the residual hexane was rem...
Assessing the in vitro toxicity of airborne (nano)particles to the human respir...
Bessa, Maria João; Brandão, Fátima; Rosário, Fernanda; Moreira, Luciana; Reis, Ana Teresa; Valdiglesias, Vanessa; Laffon, Blanca; Fraga, SóniaSeveral studies have been conducted to address the potential adverse health risks attributed to exposure to nanoscale materials. While in vivo studies are fundamental for identifying the relation-ship between dose and occurrence of adverse effects, in vitro model systems provide important information regarding the mechanism(s) of action at the molecular level. With a special focus on exposure to inhaled (nano)p...
Multiparametric in vitro genotoxicity assessment of different variants of amorp...
Brandão, Fátima; Costa, Carla; Bessa, Maria João; Valdiglesias, Vanessa; Hellack, Bryan; Haase, Andrea; Fraga, Sónia; Teixeira, João PauloThe hazard posed to human health by inhaled amorphous silica nanomaterials (aSiO2 NM) remains uncertain. Herein, we assessed the cyto- and genotoxicity of aSiO2 NM variants covering different sizes (7, 15, and 40 nm) and surface modifications (unmodified, phosphonate-, amino- and trimethylsilyl-modified) on rat alveolar epithelial (RLE-6TN) cells. Cytotoxicity was evaluated at 24 h after exposure to the aSiO2 N...
Unveiling the Toxicity of Fine and Nano-Sized Airborne Particles Generated from...
Bessa, Maria João; Brandão, Fátima; Fokkens, Paul H.B.; Leseman, Daan L.A.C.; Boere, A. John F.; Cassee, Flemming R.; Salmatonidis, ApostolosHigh-energy industrial processes have been associated with particle release into workplace air that can adversely affect workers’ health. The present study assessed the toxicity of incidental fine (PGFP) and nanoparticles (PGNP) emitted from atmospheric plasma (APS) and high-velocity oxy-fuel (HVOF) thermal spraying. Lactate dehydrogenase (LDH) release, 2-(4-nitrophenyl)-2H-5-tetrazolio]-1,3-benzene disulfonate...
Assessing the in vitro toxicity of engineered and airborne nanoceramics: contri...
Bessa, Maria JoãoAdvanced ceramic technologies have a strong potential for airborne (nano)particle formation and emission, meaning that workers of those industries are at great risk of exposure to these particles. However, toxicological data of these (nano)particles is lacking, particularly for airborne particles released within sectors such as the ceramic industry. To address this relevant topic, the present work aimed to asse...
Toxicity assessment of industrial engineered and airborne process-generated nan...
Bessa, Maria João; Brandão, Fátima; Fokkens, Paul; Cassee, Flemming R.; Salmatonidis, Apostolos; Viana, Mar; Vulpoi, Adriana; Simon, SimionThe advanced ceramic technology has been pointed out as a potentially relevant case of occupational exposure to nanoparticles (NP). Not only when nanoscale powders are being used for production, but also in the high-temperature processing of ceramic materials there is also a high potential for NP release into the workplace environment. In vitro toxicity of engineered NP (ENP) [antimony tin oxide (Sb2O3•SnO2; AT...
In Vitro Toxicity of Industrially Relevant Engineered Nanoparticles in Human Al...
Bessa, Maria João; Brandão, Fátima; Fokkens, Paul H.B.; Leseman, Daan L.A.C.; Boere, A. John F.; Cassee, Flemming R.; Salmatonidis, ApostolosDiverse industries have already incorporated within their production processes engineered nanoparticles (ENP), increasing the potential risk of worker inhalation exposure. In vitro models have been widely used to investigate ENP toxicity. Air-liquid interface (ALI) cell cultures have been emerging as a valuable alternative to submerged cultures as they are more representative of the inhalation exposure to airbo...
Genotoxicity and Gene Expression in the Rat Lung Tissue following Instillation ...
Brandão, Fátima; Costa, Carla; Bessa, Maria João; Dumortier, Elise; Debacq-Chainiaux, Florence; Hubaux, Roland; Salmon, Michel; Laloy, JulieSeveral reports on amorphous silica nanomaterial (aSiO2 NM) toxicity have been questioning their safety. Herein, we investigated the in vivo pulmonary toxicity of four variants of aSiO2 NM: SiO2_15_Unmod, SiO2_15_Amino, SiO2_7 and SiO2_40. We focused on alterations in lung DNA and protein integrity, and gene expression following single intratracheal instillation in rats. Additionally, a short-term inhalation st...
Genotoxicity of TiO2 Nanoparticles in Four Different Human Cell Lines (A549, HE...
Brandão, Fátima; Fernández-Bertólez, Natalia; Rosário, Fernanda; Bessa, Maria João; Fraga, Sónia; Pásaro, Eduardo; Teixeira, João Paulo; Laffon, BlancaTitanium dioxide nanoparticles (TiO2 NPs) have a wide variety of applications in many consumer products, including as food additives, increasing the concern about the possible hazards that TiO2 NPs may pose to human health. Although most previous studies have focused on the respiratory system, ingestion must also be considered as an important exposure route. Furthermore, after inhalation or ingestion, TiO2 NPs ...
Assessment of genotoxic effects of titanium dioxide nanoparticles on different ...
Fernández-Bertólez, Natalia; Brandao, Fátima; Rosário, Fernanda; Bessa, Maria João; Fraga, Sónia; Pásaro, Eduardo; Teixeira, Joao Paulo; Costa, CarlaThe main objective of the present work was to assess the cellular uptake and potential genotoxicity (micronuclei induction) of TiO2 NPs on four diverse human cell lines.