9 documents found, page 1 of 1
Identificação de micobactérias não tuberculosas isoladas de sítios estéreis em ...
Senna,Simone Gonçalves; Marsico,Ana Grazia; Vieira,Gisele Betzler de Oliveira; Sobral,Luciana Fonseca; Suffys,Philip Noel; Fonseca,Leila de SouzaOBJETIVO: Identificar micobactérias não tuberculosas (MNT) isoladas de sítios estéreis em pacientes internados no Hospital Universitário Clementino Fraga Filho, Rio de Janeiro (RJ) entre 2001 e 2006. MÉTODOS: Durante o período do estudo, 34 isolados de MNT de sítios estéreis de 14 pacientes, a maioria HIV positivos, foram submetidos a identificação fenotípica e hsp65 PCR-restriction enzyme analysis (PRA, anális...
An alternative for the preadsorption step in the paratuberculosis serodiagnosis...
Marassi,Carla Dray; Silva,Marley; Oelemann,Walter Martin Roland; Fonseca,Leila de Souza; Ristow,Paula; Lilenbaum,WalterELISAs for paratuberculosis employ a preadsorption step with Mycobacterium phlei to diminish unspecific reactions As M. fortuitum is one of the most frequent environmental mycobacteria, the purpose of this pilot study was to evaluate its use as an alternative for the preadsorption in ELISAs for paratuberculosis. Results suggest that M. fortuitum can be an alternative instead of or associated to M. phlei with co...
Do DNA extraction methods and Taq polimerase quality improve the double repetit...
Cavalcanti,Hebe Rodrigues; Marques,Elizabeth; Fonseca,Leila de Souza; Saad,Maria Helena FéresDouble repetitive element (DRE) PCR amplification is a simple Mycobacterium tuberculosis typing method, however amplification failure or poor resolution of bands commit its efficacy. In order to verify if whether or not these features could be minimized by improving DNA extraction procedures or Taq polymerise quality, DRE-PCR was performed on 24 M. tuberculosis DNA samples extracted by heat-shock, mechanical an...
Interference of tuberculosis on the performance of ELISAs used in the diagnosis...
Lilenbaum,Walter; Ferreira,Rachel; Marassi,Carla Dray; Ristow,Paula; Oelemann,Walter Martins Roland; Fonseca,Leila de SouzaForty-four cows from five herds infected with tuberculosis (TB) and without paratuberculosis (PTB), and 21 cows from a herd without either infection were studied. The cattle presented concordant results in both the skin test and gamma-interferon assay for TB and two commercial ELISAs for PTB. Animals were divided according to TB test results into Group A with 28 TB-infected animals, Group B with 16 TB-negative ...
Evaluation of Mycobacterium avium subsp. paratuberculosis faecal culture protoc...
Ristow,Paula; Silva,Marlei Gomes; Fonseca,Leila de Souza; Lilenbaum,WalterParatuberculosis is an important enteritis of ruminants caused by Mycobacterium avium subsp. paratuberculosis (Map). The disease is officially considered exotic in Brazil, but recent serological surveys and the isolation of the agent suggest it may occur in our herds. The aim of this study was to evaluate three different formulations of Herrold's egg yolk agar with mycobactin J (HEYM) and four faecal culture pr...
Improvement of an in-house ELISA for bovine paratuberculosis serology in Brazil
Marassi,Carla Dray; Fonseca,Leila de Souza; Ristow,Paula; Ferreira,Rachel; Lilenbaum,Walter; Oelemann,Walter M.R.Paratuberculosis (Johne's disease) is a chronic enteritis in cattle caused by infection with Mycobacterium avium subsp. paratuberculosis (Map). In Brazil, few reports describe the isolation of the organism. The gold standard test for Map is its isolation from tissues or feces. Moreover, bacterial growth is slow and test results are available only after three to five months of incubation Furthermore, shedding of...
Avaliação de testes rápidos em microplacas usando indicadores de viabilidade ce...
Ribeiro,Marta Osório; Gomes,Marlei da Silva; Senna,Simone Gonçalves; Rossetti,Maria Lucia Rosa; Fonseca,Leila de SouzaINTRODUÇÃO: As taxas de resistência aos fármacos constituem um dos pilares da avaliação dos programas de controle da tuberculose. A demora na obtenção dos resultados, conseqüência da metodologia convencional utilizada, faz com que haja a necessidade de avaliação de novos testes, mais rápidos e menos onerosos. OBJETIVO: Comparar técnicas fenotípicas rápidas para determinação do perfil de susceptibilidade de M. t...
Sensitivity of AMPLICOR MTB on direct detection of Mycobacterium tuberculosis i...
Anjos Filho,Luciano dos; Oelemann,Walter M.R.; Barreto,Clara E.N.; Kritski,Afranio Lineu; Fonseca,Leila de SouzaWe evaluated the performance of the AMPLICOR MTB assay for detection of M. tuberculosis in clinical specimens obtained from 98 smear-negative tuberculous patients (43 with positive and 55 with negative culture results). The sensitivity of the AMPLICOR MTB was 44.9% (44/98) and that of culture was 43.8% (43/98). No significant difference was observed between the results obtained by AMPLICOR MTB and by culture. W...
Relação entre o diagnóstico sorológico (ELISA) e a gravidade da tuberculose pul...
Sant'Anna,Clemax Couto; Fonseca,Leila de Souza; Saad,Maria Helena FéresForam estudadas 48 crianças de 0 a 13 anos através da realização do ensaio imunoenzimático ligado a enzima (ELISA) para pesquisa de anticorpos da classe IgG antiPPD, visando estabelecer correlação entre a resposta imune humoral medida pela sorologia e a gravidade da tuberculose, segundo formas radiológicas (leve, moderada e grave). A amostra foi composta de 29 crianças com tuberculose e 19 sem tuberculose comun...