6 documents found, page 1 of 1
Impact of Conventional and Potential New Metal-Based Drugs on Lipid Metabolism ...
Bispo, Daniela S. C.; Correia, Marlene; Carneiro, Tatiana J.; Martins, Ana S.; Reis, Aliana A. N.; Carvalho, Ana L. M. Batista de; Marques, Maria P. M.This work investigated the mechanisms of action of conventional drugs, cisplatin and oxaliplatin, and the potentially less deleterious drug Pd2Spermine (Spm) and its Pt(II) analog, against osteosarcoma MG-63 cells, using nuclear-magnetic-resonance metabolomics of the cellular lipidome. The Pt(II) chelates induced different responses, namely regarding polyunsaturated-fatty-acids (increased upon cisplatin), sugge...
Impact of Sea Warming and 17-α-Ethinylestradiol Exposure on the Lipid Metabolis...
Rodrigues, João A.; Bispo, Daniela S. C.; Silva, Mónica G.; Araújo, Rita; Soares, Amadeu M. V. M.; Freitas, Rosa; Gil, Ana M.This paper reports on an NMR metabolomics study of lipophilic extracts of Ruditapes philippinarum clams exposed to the hormonal contaminant 17-α-ethinylestradiol (EE2), at 17 °C and 21 °C. The results reveal that exposure at 17 °C triggers a weak response at low EE2 concentrations, suggestive of a slight increase in membrane rigidity, followed by lipid metabolic stability at higher EE2 concentrations. On the ot...
NMR metabolomics assessment of osteogenic differentiation of adipose-tissue-der...
Bispo, Daniela S. C.; Jesus, Catarina S. H.; Correia, Marlene; Ferreira, Filipa; Bonifazio, Giulia; Goodfellow, Brian J.; Oliveira, Mariana B.This Article presents, for the first time to our knowledge, an untargeted nuclear magnetic resonance (NMR) metabolomic characterization of the polar intracellular metabolic adaptations of human adipose-derived mesenchymal stem cells during osteogenic differentiation. The use of mesenchymal stem cells (MSCs) for bone regeneration is a promising alternative to conventional bone grafts, and untargeted metabolomics...
Endo- and exometabolome crosstalk in mesenchymal stem cells undergoing osteogen...
Bispo, Daniela S. C.; Michálková, Lenka; Correia, Marlene; Jesus, Catarina S. H.; Duarte, Iola F.; Goodfellow, Brian J.; Oliveira, Mariana B.This paper describes, for the first time to our knowledge, a lipidome and exometabolome characterization of osteogenic differentiation for human adipose tissue stem cells (hAMSCs) using nuclear magnetic resonance (NMR) spectroscopy. The holistic nature of NMR enabled the time-course evolution of cholesterol, mono- and polyunsaturated fatty acids (including ω-6 and ω-3 fatty acids), several phospholipids (phosph...
An intracellular metabolic signature as a potential donor-independent marker of...
Bispo, Daniela S. C.; Jesus, Catarina S. H.; Romek, Katarzyna; Marques, Inês M. C.; Oliveira, Mariana B.; Mano, João F.; Gil, Ana M.This paper describes an untargeted NMR metabolomics study to identify potential intracellular donor-dependent and donor-independent metabolic markers of proliferation and osteogenic differentiation of human adipose mesenchymal stem cells (hAMSCs). The hAMSCs of two donors with distinct proliferating/osteogenic characteristics were fully characterized regarding their polar endometabolome during proliferation and...
Metabolomic applications in stem cell research: a review
Bispo, Daniela S. C.; Jesus, Catarina S. H.; Marques, Inês M. C.; Romek, Katarzyna M.; Oliveira, Mariana B.; Mano, João F.; Gil, Ana M.This review describes the use of metabolomics to study stem cell (SC) characteristics and function, excluding SCs in cancer research, suited to a fully dedicated text. The interest in employing metabolomics in SC research has consistently grown and emphasis is, here, given to developments reported in the past five years. This text informs on the existing methodologies and their complementarity regarding the inf...