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Influence of forskolin supplementation on embryos produced in vitro
Costa, Camila Bortoliero; Lunardelli, Paula Alvares; Bizarro-Silva, Camila; Fontes, Patricia Kubo [UNESP]; Nogueira, Marcelo Fábio Gouveia [UNESP]Made available in DSpace on 2019-10-06T16:59:55Z (GMT). No. of bitstreams: 0 Previous issue date: 2019-03-01; The objective of this study was to evaluate the effects of forskolin supplementation of the culture medium on the development, lipid content, and transcript levels genes of in vitro-produced bovine embryos. Ovaries (n = 420) from Nelore cows were obtained from the slaughterhouse. On day 5 (D5) of in vit...
Proliferative activity of oocytes in multi-oocyte follicles of bovine ovary
Oliveira, Reginaldo Luis; Silva-Santos, Katia Cristina; Gonzalez, Suellen Miguez; Bizarro-Silva, Camila; Machado, Fernanda ZandonadiWe characterized the proliferative activity of multi-oocyte follicles with anti-nuclear antigen of proliferating cells (PCNA). Ovaries (n = 12) from heifers were processed for histology. From 789 multi-oocytes follicles observed, only 11 were considered appropriated for immunostaining, since they presented all nuclei of the oocytes clearly visible. All multi-oocyte follicles were positive for PCNA, but some ooc...