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Alternative methodologies for genotyping polymorphisms in the CAST and CAPN1 ge...

Blecha,Isabella Maiumi Zaidan; Sousa,Isadora Inácio; Ferreira,Anna Beatriz Robottom; Feijó,Gelson Luis Dias; Torres Júnior,Roberto Augusto de Almeida

ABSTRACT The objectives of this study were to genotype single nucleotide polymorphisms (SNP) AF159246:g.2959A>G (CAST/DdeI) and AF248054.2:g.6545C>T (CAPN4751) in beef cattle by PCR-RFLP (Polymerase Chain Reaction - Restriction Fragment Length Polymorphism), using the restriction enzyme DdeI for both SNP, and describe the use of these genotyping methodologies for the first time. For the SNP located in the CAST ...

Date: 2019   |   Origin: Oasisbr

Wildlife species, Ixodid fauna and new host records for ticks in an Amazon fore...

Zimmermann,Namor Pinheiro; Aguirre,André de Abreu Rangel; Rodrigues,Vinicius da Silva; Garcia,Marcos Valério; Medeiros,Jansen Fernandes

Abstract The objective of this work was to evaluate the diversity of ticks associated with free-living animals and to investigate new host records for ticks. Ticks were collected from animals rescued during the flood of the Jamari River in the municipality of Ariquemes, state of Rondônia, North Region of Brazil. A total of 39 animals were captured, out of which 10 were amphibians, 19 were reptiles and 10 were m...

Date: 2018   |   Origin: Oasisbr

Analysis of Bm86 conserved epitopes: is a global vaccine against Cattle Tick Rh...

Blecha,Isabella Maiumi Zaidan; Csordas,Bárbara Guimarães; Aguirre,André de Abreu Rangel; Cunha,Rodrigo Casquero; Garcia,Marcos Valério; Andreotti,Renato

Abstract The cattle tick Rhipicephalus microplus causes significant economic losses in agribusiness. Control of this tick is achieved mainly through the application of chemical acaricides, often resulting in contamination of animal food products and of the environment. Another major concern associated with acaricide use is the increasing reports of resistance of this tick vector against the active ingredients o...

Date: 2018   |   Origin: Oasisbr

New insights from molecular characterization of the tick Rhipicephalus (Boophil...

Csordas,Bárbara Guimarães; Garcia,Marcos Valério; Cunha,Rodrigo Casquero; Giachetto,Poliana Fernanda; Blecha,Isabella Maiumi Zaidan; Andreotti,Renato

Abstract The Rhipicephalus (Boophilus) microplus complex currently consists of five taxa, namely R. australis, R. annulatus, R. (B.) microplus clade A sensu, R. microplus clade B sensu, and R. (B.) microplus clade C sensu. Mitochondrial DNA-based methods help taxonomists when they are facing the morpho-taxonomic problem of distinguishing members of the R. (B.) microplus complex. The purpose of this study was to...

Date: 2016   |   Origin: Oasisbr

Association of polymorphisms in the leptin and thyroglobulin genes with meat qu...

Carvalho,Thiago Dutra de; Siqueira,Fabiane; Torres Júnior,Roberto Augusto de Almeida; Medeiros,Sérgio Raposo de; Feijó,Gelson Luís Dias

The objective of the present study was to estimate the allelic and genotypic frequencies of the polymorphisms E2FB (AY138588.1: c.305C> T), located in the leptin gene (LEP), and TG5 (X05380.1:g.-422C>T), located in the thyroglobulin gene (TG), and evaluate the association of these polymorphisms in crossbred cattle of seven distinct genetic groups with the following traits: slaughter weight (SW), hot carcass wei...

Date: 2012   |   Origin: Oasisbr

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