4 documents found, page 1 of 1
Ion-exchange chromatography used to isolate a spermadhesin-related protein from...
Teixeira, Dárcio Ítalo Alves; Melo, Luciana Magalhães; Gadelha, Carlos Alberto de Almeida; Cunha, Rodrigo Maranguape Silva da; Bloch Júnior, CarlosMammalian seminal plasma contains among others, proteins called spermadhesins, which are the major proteins of boar and stallion seminal plasma. These proteins appear to be involved in capacitation and sperm-egg interaction. Previously, we reported the presence of a protein related to spermadhesins in goat seminal plasma. In the present study, we have further characterized this protein, and we propose ion-excha...
Phylloseptin-1 is leishmanicidal for amastigotes of Leishmania amazonensis insi...
Kückelhaus, Selma Aparecida Souza; Aquino, Daniela Sant’Ana de; Borges, Tatiana Karla dos Santos; Moreira, Daniel Carneiro; Leite, Luciana de MagalhãesLeishmania protozoans are the causal agents of neglected diseases that represent an important public health issue worldwide. The growing occurrence of drug-resistant strains of Leishmania and severe side effects of available treatments represent an important challenge for the leishmaniases treatment. We have previously reported the leishmanicidal activity of phylloseptin-1 (PSN-1), a peptide found in the skin s...
Cinerascetins, new peptides from Hypsiboas cinerascens : MALDI LIFT-TOF-MS/MS d...
Almeida, Richardson Alves de; Gordo, Marcelo; Silva, Felipe Moura Araujo da; Araújo, Rafael C. de; Ramada, Marcelo Henrique Soller; Abrão, Fernando YanoThe continuous search for antimicrobial candidates pushes the pursuit of compounds in the most diverse organisms. Amphibians are known as a prolific source of antibacterial peptides. Based on the rich biodiversity of the Amazon region the unexplored green-tree frog (Hypsiboas cinerascens) was studied for its skin secretion peptide content. Chromatographic separations and established tandem mass spectrometry (MS...
Cloning, purification, and partial characterization of Bacillus subtilis urate ...
Pfrimer, Pollyanna; Moraes, Lídia Maria Pepe de; Galdino, Alexsandro Sobreira; Salles, Loise Pedrosa; Reis, Viviane Castelo BrancoUrate oxidase (EC 1.7.3.3) is an enzyme involved in purine metabolism which is used in the treatment of gout and as diagnostic reagent for detection of uric acid. In order to produce this enzyme in large quantities for biotechnological purposes, the gene coding for the Bacillus subtilis urate oxidase was cloned and heterologously expressed in Escherichia coli. Time course induction in E. coli showed an induced ...