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Superior operational stability of immobilized l-asparaginase over surface-modif...
Almeida, Mafalda R.; Cristóvão, Raquel O.; Barros, Maria A.; Nunes, João C. F.; Boaventura, Rui A. R.; Loureiro, José M.; Faria, Joaquim L.Made available in DSpace on 2022-04-28T19:46:42Z (GMT). No. of bitstreams: 0 Previous issue date: 2021-12-01; Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP); Fundação para a Ciência e a Tecnologia; l-asparaginase (ASNase, EC 3.5.1.1) is an enzyme that catalyzes the l-asparagine hydrolysis into l-aspartic acid and ammonia, being mainly applied in pharmaceutical and food industries. However, some d...
Superior operational stability of immobilized L-asparaginase over surface-modif...
Almeida, Mafalda R.; Cristóvão, Raquel O.; Barros, Maria A.; Nunes, João C. F.; Boaventura, Rui A. R.; Loureiro, José M.; Faria, Joaquim L.L-asparaginase (ASNase, EC 3.5.1.1) is an enzyme that catalyzes the L-asparagine hydrolysis into L-aspartic acid and ammonia, being mainly applied in pharmaceutical and food industries. However, some disadvantages are associated with its free form, such as the ASNase short half-life, which may be overcome by enzyme immobilization. In this work, the immobilization of ASNase by adsorption over pristine and modifi...
Immobilization of L-asparaginase towards surface-modified carbon nanotubes
Almeida, Mafalda R.; Cristóvão, Raquel O.; Barros, Maria A.; Nunes, João C.F.; Boaventura, Rui A. R.; Loureiro, José M.; Faria, Joaquim L.L-asparaginase (ASNase, EC 3.5.1.1) is an enzyme that catalyzes L-asparagine hydrolysis into L-aspartic acid and ammonia and is mainly applied in pharmaceutical and food industries [1]. The ASNase currently commercialized for pharmaceutical purposes is produced from two main bacterial sources: recombinant Escherichia coli and Erwinia chrysanthemi. However, some disadvantages are associated with its free form, s...
Development and characterization of a novel l-asparaginase/MWCNT nanobioconjugate
Cristovao, Raquel O.; Almeida, Mafalda R.; Barros, Maria A.; Nunes, Joao C. F.; Boaventura, Rui A. R.; Loureiro, Jose M.; Faria, Joaquim L.Made available in DSpace on 2020-12-12T01:38:47Z (GMT). No. of bitstreams: 0 Previous issue date: 2020-08-24; The enzyme l-asparaginase (ASNase) presents effective antineoplastic properties used for acute lymphoblastic leukemia treatment besides their potential use in the food sector to decrease the acrylamide formation. Considering their applications, the improvement of this enzyme's properties by efficient im...
Development and characterization of a novel l-asparaginase/MWCNT nanobioconjugate
Cristóvão, Raquel O.; Almeida, Mafalda R.; Barros, Maria A.; Nunes, João C. F.; Boaventura, Rui A. R.; Loureiro, José M.; Faria, Joaquim L.The enzyme L-asparaginase (ASNase) presents effective antineoplastic properties used for acute lymphoblastic leukemia treatment besides their potential use in the food sector to decrease the acrylamide formation. Considering their applications, the improvement of this enzyme's properties by efficient immobilization techniques is in high demand. Carbon nanotubes are promising enzyme immobilization supports, sinc...
Use of cork granules as an effective sustainable material to clean-up spills of...
Todescato, Diego; Hackbarth, Fabíola V.; Carvalho, Pedro J.; Souza, Antônio A. Ulson de; Souza, Selene M A. G. Ulson de; Boaventura, Rui A. R.The use of cork granules for cleaning up crude oil or oil derivative spills and further oil recovery appears as a promising option due to their unique properties, which allow a high oil sorption capacity, low water pickup and excellent reuse. The present work reports the effect of oil viscosity on cork sorption capacity by using five types of oils (lubricating oil, 5.7 goil gcork-1; heavy oil, 4.2 goil gcork-1;...
Combination of chemical coagulation, photo-Fenton oxidation and biodegradation ...
Rodrigues, Carmen S. D.; Neto, Ana R.; Duda, Rose M.; Oliveira, Roberto A. de [UNESP]; Boaventura, Rui A. R.; Madeira, Luis M.Made available in DSpace on 2018-11-26T17:20:53Z (GMT). No. of bitstreams: 0 Previous issue date: 2017-01-20; FEDER; national funds through FCT - Fundacaip para a Ciencia e a Tecnologia; LEPABE; Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES); Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq); The post-treatment of biodigested sugarcane vinasse was envisaged in the present...
Water quality modelling of Lis River, Portugal
Vieira, Judite; Fonseca, André; Vilar, Vítor J. P.; Boaventura, Rui A. R.; Botelho, Cidália M. S.The aim of the study was to predict the impact of flow conditions, discharges and tributaries on the water quality of Lis River using QUAL2Kw model. Calibration of the model was performed, based on data obtained in field surveys carried out in July 2004 and November 2006. Generally the model fitted quite well the experimental data. The results indicated a decrease of water quality in the downstream area of Lis ...
Effect of Cr (VI) in Four Portuguese Microalgae Growth
Dinis, Maria Alzira Pimenta; Vilar, Vitor J. P.; Monteiro, Álvaro; Boaventura, Rui A. R.The objective of this work was to study the growth of microalgae Chlorella fusca ACOI 621, Chlorella vulgaris ACOI 879, Scenedesmus acutus ACOI 538 and Scenedesmus obliquus ACOI 550, in the presence and absence of Cr(VI). The microalgae growth was assessed as a function of different parameters, particularly, temperature, pH and Cr(VI) addition. Although growth inhibition due to Cr(VI) toxicity is rather visible...
Surface Water Quality Assessment of Lis River Using Multivariate Statistical Me...
Vieira, Judite S.; Pires, José C. M.; Martins, Fernando G.; Vilar, Vítor J. P.; Boaventura, Rui A. R.; Botelho, Cidália M. S.This study presents the application of multivariate statistical tools for the evaluation of spatial variations and the interpretation of water quality data obtained in a monitoring program of Lis river basin surface water, Portugal. Twenty-seven physicochemical and microbiological parameters were determined in six water sampling campaigns at 16 monitoring sites during the period from September 2003 to November ...