15 documents found, page 1 of 2
Genomic instability as a main driving factor of unsuccessful ageing: Potential ...
Laffon, B; Bonassi, S; Costa, S; Valdiglesias, VGenome instability denotes an increased tendency to alterations in the genome during cell life cycle, driven by a large variety of endogenous and exogenous insults. Ageing is characterized by the presence of damage to various cellular constituents, but genome alterations, randomly accumulating with age in different tissues, constitute the key target in this process, and are believed to be the main factor of age...
DNA damage in circulating leukocytes measured with the comet assay may predict ...
Bonassi, S; Ceppi, M; Møller, P; Azqueta, A; Milić, M; Neri, M; Brunborg, G; Godschalk, R; Koppen, G; Langie, SAS; Teixeira, JP; Bruzzone, MThe comet assay or single cell gel electrophoresis, is the most common method used to measure strand breaks and a variety of other DNA lesions in human populations. To estimate the risk of overall mortality, mortality by cause, and cancer incidence associated to DNA damage, a cohort of 2,403 healthy individuals (25,978 person-years) screened in 16 laboratories using the comet assay between 1996 and 2016 was fol...
The hCOMET project: International database comparison of results with the comet...
Milić, M; Ceppi, M; Bruzzone, M; Azqueta, A; Brunborg, G; Godschalk, R; Koppen, G; Langie, S; Møller, P; Teixeira, JP; Alija, A; Anderson, D; Andrade, VThe alkaline comet assay, or single cell gel electrophoresis, is one of the most popular methods for assessing DNA damage in human population. One of the open issues concerning this assay is the identification of those factors that can explain the large inter-individual and inter-laboratory variation. International collaborative initiatives such as the hCOMET project - a COST Action launched in 2016 - represent...
Low Vitamin D Levels and Frailty Status in Older Adults: A Systematic Review an...
Marcos-Pérez, D; Sánchez-Flores, M; Proietti, S; Bonassi, S; Costa, S; Teixeira, JP; Fernández-Tajes, J; Pásaro, E; Valdiglesias, V; Laffon, BSerum vitamin D deficiency is widespread among older adults and is a potential modifiable risk factor for frailty. Moreover, frailty has been suggested as an intermediate step in the association between low levels of vitamin D and mortality. Hence, we conducted a systematic review of the literature and meta-analysis to test the possible association of low concentrations of serum 25-hydroxyvitamin D (25(OH)D), a...
Minimum Information for Reporting on the Comet Assay (MIRCA): recommendations f...
Møller, P; Azqueta, A; Boutet-Robinet, E; Koppen, G; Bonassi, S; Milić, M; Gajski, G; Costa, S; Teixeira, JP; Costa Pereira, C; Dusinska, MThe comet assay is a widely used test for the detection of DNA damage and repair activity. However, there are interlaboratory differences in reported levels of baseline and induced damage in the same experimental systems. These differences may be attributed to protocol differences, although it is difficult to identify the relevant conditions because detailed comet assay procedures are not always published. Here...
Association of inflammatory mediators with frailty status in older adults: resu...
Marcos-Pérez, D; Sánchez-Flores, M; Proietti, S; Bonassi, S; Costa, S; Teixeira, JP; Fernández-Tajes, J; Pásaro, E; Laffon, B; Valdiglesias, VFrailty is a geriatric syndrome defined as a status of extreme vulnerability to stressors, leading to a higher risk of negative health-related outcomes. “Inflammaging”, an age-related state of low-grade chronic inflammation, is characterized by an increased concentration of pro-inflammatory cytokines and acute phase proteins. Inflammaging has been postulated as an underlying mechanism of frailty, and several st...
Immunological alterations in frail older adults: A cross sectional study
Valdiglesias, V; Marcos-Perez, D; Lorenzi, M; Onder, G; Gostner, JM; Strasser, B; Fuchs, D; Bonassi, SFrailty is a progressive physiologic decline in multiple body systems, characterized by loss of function, loss of physiologic reserve, and increased vulnerability to disease and death. This condition is induced by a complex and multifactorial interaction between genetic, biological, physical, psychological and environmental factors. To understand the interplay between the age-related decline of the immune respo...
Frailty Syndrome and Genomic Instability in Older Adults: Suitability of the Cy...
Sanchez-Flores, M; Marcos-Perez, D; Lorenzo-Lopez, L; Maseda, A; Millan-Calenti, JC; Bonassi, S; Pasaro, E; Laffon, B; Valdiglesias, VFrailty, a condition involving increased risk of disability and mortality in older adults, has emerged as a reliable way to predict the effect of aging. Genomic instability may help to anticipate recognition of frail individuals and improving frailty outcomes. Our objective was to evaluate the potential of the micronucleus frequency, evaluated in lymphocytes and buccal cells, to anticipate frailty identificatio...
Oxidative stress, genomic features and DNA repair in frail elderly: a systemati...
Sánchez-Flores, M; MArcos-Pérez, D; Costa, S; Teixeira, JP; Bonassi, S; Pásaro, E; Laffon, B; Valdiglesias, VFrailty is an emerging geriatric syndrome characterized by higher vulnerability to stressors, with an increased risk of adverse health outcomes such as mortality, morbidity, disability, hospitalization, and institutionalization. Although it is generally recognized to have a biological basis, no particular biological trait has been consistently associated to frailty status so far. In this work, epidemiological s...
The Influence of Thermal Comfort on the Quality of Life of Nursing Home Residents
Mendes, A; Papoila, AL; Carreiro-Martins, P; Aguiar, L; Bonassi, S; Caires, I; Palmeiro, T; Ribeiro, AS; Neves, P; Pereira, C; Botelho, A; Neuparth, NThermal comfort (TC) parameters were measured in 130 rooms from nursing homes (NH), following ISO 7730:2005 in order to evaluate the influence of winter season TC indices on quality of life (QoL) in older individuals. Mean radiant temperature (mrT), predicted mean vote (PMV) and predicted percent of dissatisfied people (PPD) indices, and the respective measurement uncertainties were calculated using Monte Carlo...