2 documents found, page 1 of 1
The role of vitamin C in the gene expression of oxidative stress markers in fib...
Bonucci,Jessica; Gragnani,Alfredo; Trincado,Marcelo Moraes; Vincentin,Victor; Correa,Silvana Aparecida Alves; Ferreira,Lydia MasakoAbstract Purpose: To assess the action of vitamin C on the expression of 84 oxidative stress related-genes in cultured skin fibroblasts from burn patients. Methods: Skin samples were obtained from ten burn patients. Human primary fibroblasts were isolated and cultured to be distributed into 2 groups: TF (n = 10, fibroblasts treated with vitamin C) and UF (n = 10, untreated fibroblasts). Gene expression analysis...
Aldefluor protocol to sort keratinocytes stem cells from skin
Noronha,Samuel Marcos Ribeiro; Gragnani,Alfredo; Pereira,Thiago Antônio Calado; Correa,Silvana Aparecida Alves; Bonucci,Jessica; Ferreira,Lydia MasakoAbstract Purpose: To investigate the use Aldefluor® and N, N - Dimethylaminobenzaldehyde (DEAB) to design a protocol to sort keratinocyte stem cells from cultured keratinocytes from burned patients. Methods: Activated Aldefluor® aliquots were prepared and maintained at temperature between 2 to 8°C, or stored at -20°C. Next, the cells were collected following the standard protocol of sample preparation. Results:...