15 documents found, page 1 of 2
APOBEC3 deaminase editing in mpox virus as evidence for sustained human transmi...
O’Toole, Áine; Neher, Richard A.; Ndodo, Nnaemeka; Borges, Vitor; Gannon, Ben; Gomes, João Paulo; Groves, Natalie; King, David J.; Maloney, DanielHistorically, mpox has been characterized as an endemic zoonotic disease that transmits through contact with the reservoir rodent host in West and Central Africa. However, in May 2022, human cases of mpox were detected spreading internationally beyond countries with known endemic reservoirs. When the first cases from 2022 were sequenced, they shared 42 nucleotide differences from the closest mpox virus (MPXV) p...
Prospective observational study on the role of catheter colonization and multid...
Pinto, Miguel; Borges, Vitor; Nascimento, Maria; Martins, Filomena; Pessanha, Maria Ana; Faria, Isabel; Rodrigues, Joao; Matias, Rui; Joao Paulo, GomesBackground: Central venous catheter (CVC)-related bloodstream infection (CRBSI) is a huge public health consern with considerable impact on mortality and health costs. The emergence of antimicrobial resistant microorganisms associated or not with CVC colonization by biofilms makes the treatment of CRBSI even more challeging. Methods:A 3-year observational study enrolling 3 tertiary hospitals located in Lisbon (...
Chlamydia trachomatis outbreak: when the virulence-associated genome backbone i...
Borges, Vitor; Cordeiro, Dora; Salas, Ana Isabel; Lodhia, Zohra; Correia, Cristina; Isidro, Joana; Fernandes, Cândida; Rodrigues, Ana; Azevedo, JacintaChlamydia trachomatis is the most prevalent sexually transmitted bacterium worldwide and the causative agent of trachoma. Its strains are classified according to their ompA genotypes, which are strongly linked to differential tissue tropism and disease outcomes [ocular disease, urogenital disease and lymphogranuloma venereum (LGV)]. While the genome-based species phylogenetic tree presents four main clades corr...
INSaFLU: a bioinformatics framework that potentiates the integration of influen...
Borges, VitorProcurarei dar um perspetiva geral da importância da análise total do genoma viral na vigilância laboratorial (e actividades de I&D) do vírus influenza, destacando o potencial papel da plataforma INSaFLU nesta revolução tecnológica / científica. Abordarei ainda alguns desafios (e perspetivas futuras) associados à integração da informação gerada com a sequenciação total do genoma de agentes patogénicos na descod...
Phenotypic signatures and genetic determinants of oxacillin tolerance in a labo...
Chung, Marilyn; Borges, Vitor; Gomes, João Paulo; de Lencastre, Herminia; Tomasz, AlexanderAddition of β-lactam antibiotics to growing cultures of bacteria inhibit synthesis of the bacterial cell wall peptidoglycan accompanied by killing (loss of viable titer) and lysis (physical disintegration) of the cells. However, it has also been well established that these antibiotics are not effective in killing non-growing or slow-growing bacteria and the mechanism of this "antibiotic tolerance" is not well u...
Genetic Determinants of High-Level Oxacillin Resistance in Methicillin-Resistan...
Pardos de la Gandara, Maria; Borges, Vitor; Chung, Marilyn; Milheiriço, Catarina; Gomes, João Paulo; de Lencastre, Herminia; Tomasz, AlexanderMethicillin-resistant Staphylococcus aureus (MRSA) strains carry either a mecA- or a mecC-mediated mechanism of resistance to beta-lactam antibiotics, and the phenotypic expression of resistance shows extensive strain-to-strain variation. In recent communications, we identified the genetic determinants associated with the stringent stress response that play a major role in the antibiotic resistant phenotype of ...
A large outbreak of Legionnaires’ Disease in an industrial town in Portugal
George, Francisco; Shivaji, Tara; Pinto, Catia Sousa; Serra, Luis Antonio Oliveira; Valente, João; Albuquerque, Maria JoãoBackground: We describe the investigation and control of an outbreak of Legionnaires’ disease in Portugal in October, November and December 2014. Methods: Confirmed cases were individuals with pneumonia, laboratory evidence of Legionella pneumophila serogroup 1 and exposure, by residence, occupational or leisure to the affected municipalities. 49 possible sources were reduced to four potential sources, all indu...
Multilocus sequencing typing as a tool to investigate childhood Haemophilus inf...
Bettencourt, Célia; Borges, Vitor; Gonçalo-Marques, José; Cunha, Florbela; Bajanca-Lavado, Maria PaulaBackground: Haemophilus influenzae is an important cause of serious childhood invasive disease despite the use of the vaccine against serotype b strains (Hib). Six capsular types, a-f, have been identified to date, although most of strains are non-capsulated (NC). Multilocus Sequencing Typing (MLST) is a powerful method that allows a precise and unambiguous characterization of H. influenzae genotypes. A partner...
A large outbreak of Legionnaires’ Disease in an industrial town in Portugal
George, Francisco; Shivaji, Tara; Pinto, Catia Sousa; Serra, Luis Antonio Oliveira; Valente, João; Albuquerque, Maria JoãoA B S T R A C T - Background: We describe the investigation and control of an outbreak of Legionnaires’ disease in Portugal in October, November and December 2014. Methods: Confirmed cases were individuals with pneumonia, laboratory evidence of Legionella pneumophila serogroup 1 and exposure, by residence, occupational or leisure to the affected municipalities. 49 possible sources were reduced to four potential...
A large outbreak of Legionnaires’ Disease in an industrial town in Portugal
George, Francisco; Shivaji, Tara; Pinto, Catia Sousa; Serra, Luis Antonio Oliveira; Valente, João; Albuquerque, Maria JoãoABSTRACT - Background: We describe the investigation and control of an outbreak of Legionnaires’ disease in Portugal in October, November and December 2014. Methods: Confirmed cases were individuals with pneumonia, laboratory evidence of Legionella pneumophila serogroup 1 and exposure, by residence, occupational or leisure to the affected municipalities. 49 possible sources were reduced to four potential source...