28 documents found, page 1 of 3
Diagnosis of Schistosoma mansoni infections : what are the choices in Brazilian...
Moraes, Vanessa Silva; Shollenberger, Lisa Marie; Siqueira, Liliane Maria Vidal; Borges, William de Castro; Harn, Donald A.The population of Brazil is currently characterised by many individuals harbouring low-intensity Schistosoma mansoni infections. The Kato-Katz technique is the diagnostic method recommended by the World Health Organization (WHO) to assess these infections, but this method is not sensitive enough in the context of low egg excretion. In this regard, potential alternatives are being employed to overcome the limits...
Serological proteomic screening and evaluation of a recombinant egg antigen for...
Moraes, Vanessa Silva; Shollenberger, Lisa Marie; Borges, William de Castro; Rabello, Ana Lúcia Teles; Harn, Donald A.Background: Despite decades of use of control programs, schistosomiasis remains a global public health problem. To further reduce prevalence and intensity of infection, or to achieve the goal of elimination in low-endemic areas, there needs to be better diagnostic tools to detect low-intensity infections in low-endemic areas in Brazil. The rationale for development of new diagnostic tools is that the current st...
Differential expression of proteins in genetically distinct Trypanosoma cruzi s...
Oliveira, Maykon Tavares de; Rúbio, Karina Taciana Santos; Neves, Leandro Xavier; Toledo, Max Jean de Ornelas; Borges, William de Castro; Lana, Marta deBackground Trypanosoma cruzi, a hemoflagellate protozoan parasite and the etiological agent of Chagas disease (CD), exhibits great genetic and biological diversity. Infected individuals may present clinical manifestations with different levels of severity. Several hypotheses have been proposed to attempt to correlate the diversity of clinical signs and symptoms to the genetic variability of T. cruzi. This work ...
The schistosomiasis spleenOME : unveiling the proteomic landscape of splenomega...
Cosenza Contreras, Miguel de Jesus; Castro, Renata Alves de Oliveira e; Mattei, Bruno; Campos, Jonatan Marques; Silva, Gustavo GonçalvesSchistosomiasis is a neglected parasitic disease that affects millions of people worldwide and is caused by helminth parasites from the genus Schistosoma. When caused by S. mansoni, it is associated with the development of a hepatosplenic disease caused by an intense immune response to the important antigenic contribution of adult worms and to the presence of eggs trapped in liver tissue. Although the importanc...
Alterations in the proteomic composition of Serratia marcescens in response to ...
Queiroz, Pollyana Santos; Ruas, France Anne Dias; Barboza, Natália Rocha; Borges, William de Castro; Cota, Renata Guerra de SáBackground: Proteomics is an important tool for the investigation of dynamic physiological responses of microbes under heavy metal stress. To gain insight into how bacteria respond to manganese (II) and identify the proteins involved in Mn (II) oxidation, the shotgun proteomics approach was applied to a potential Mn (II)-oxidizing Serratia marcescens strain cultivated in the absence and presence of Mn (II). Res...
Microexon gene transcriptional profiles and evolution provide insights into blo...
Li, Xiao Hong; DeMarco, Ricardo; Neves, Leandro Xavier; James, Sally R.; Newling, Katherine; Ashton, Peter D.; Cao, Jian Ping; Wilson, R. AlanAdult schistosomes have a well-developed alimentary tract comprising an oral sucker around the mouth, a short esophagus and a blind ending gut. The esophagus is not simply a muscular tube for conducting blood from the mouth to gut but is divided into compartments, surrounded by anterior and posterior glands, where processing of ingested blood is initiated. Self-cure of rhesus macaques from a Schistosoma japonic...
Transcriptional profile and structural conservation of SUMO-Specific proteases ...
Pereira, Roberta Verciano; Cabral, Fernanda Janku; Gomes, Matheus de Souza; Passos, Liana Konovaloff Jannotti; Borges, William de CastroSmall ubiquitin-related modifier (SUMO) is involved in numerous cellular processes including protein localization, transcription, and cell cycle control. SUMOylation is a dynamic process, catalyzed by three SUMO-specific enzymes and reversed by Sentrin/SUMO-specific proteases (SENPs). Here we report the characterization of these proteases in Schistosoma mansoni. Using in silico analysis, we identified two SENPs...
Exposure of cultured fibroblasts to the peptide PR-11 for the identification of...
Breguez, Gustavo Silveira; Neves, Leandro Xavier; Rúbio, Karina Taciana Santos; Freitas, Lorran Miranda Andrade de; Faria, Gabriela de OliveiraThe PR-11 peptide corresponds to the N-terminal and active region of the endogenously synthesized PR-39 molecule, of porcine origin. It is known to possess various biological effects including antimicrobial properties, angiogenic and anti-inflammatory activities. Apart from its reported activity as a proteasome inhibitor, a more comprehensive understanding of its function, at the molecular level, is still lacki...
Plasma membrane proteomes of differentially matured dendritic cells identified ...
Bernard, Stéphanie Ferret; Borges, William de Castro; Dowle, Adam A.; Sanin, David E.; Cook, Peter C.; Turner, Joseph D.; MacDonald, Andrew S.Dendritic cells (DCs) play a pivotal role in polarising Th lymphocyte subsets but it is unclear whatmolecular events occur when DCs generate Th2-type responses. Here, we analysed plasma membrane-enriched fractions from immature, pro-Th1 and pro-Th2 DCs and used a combination of iTRAQ labelling and LC–MS/MS to quantify changes in the proteomes. Analysis was performed on triplicate biological samples and changes ...
Understanding global changes of the liver proteome during murine schistosomiasi...
Campos, Jonatan Marques; Neves, Leandro Xavier; Paiva, Nívia Carolina Nogueira de; Castro, Renata Alves de Oliveira e; Casé, Ana HelenaSchistosomiasis is an endemic disease affecting over 207million people worldwide caused by helminth parasites of the genus Schistosoma. In Brazil the disease is responsible for the loss of up to 800 lives annually, resulting from the desabilitating effects of this chronic condition. In this study, we infected Balb/c mice with Schistosoma mansoni and analysed global changes in the proteomic profile of soluble li...