5 documents found, page 1 of 1
Prevalence of four urogenital sexually transmitted infections in a dedicated cl...
Lodhia, Zohra; Azevedo, Jacinta; Alves, João; Cordeiro, Dora; Antunes, Marília; Borrego, Maria J.Background/Objectives: To determine the prevalence of urogenital Chlamydia trachomatis (CT), Neisseria gonorrhoeae (NG), Trichomonas vaginalis (TV), and Mycoplasma genitalium (MG) among attendees of an open and freely available sexually transmitted infections (STI) dedicated clinic in Lisbon, at Centro de Saúde da Lapa, during 1-year. Methods: Molecular testing for CT, NG, MG, and TV was performed on 1,062 urog...
Parallel Evolution of Group B Streptococcus Hypervirulent Clonal Complex 17 Unv...
Almeida, Alexandre; Rosinski-Chupin, Isabelle; Plainvert, Céline; Douarre, Pierre-Emmanuel; Borrego, Maria J.; Poyart, Claire; Glaser, PhilippeGroup B Streptococcus (GBS) is a commensal of the gastrointestinal and genitourinary tracts, while a prevailing cause of neonatal disease worldwide. Of the various clonal complexes (CCs), CC17 is overrepresented in GBS-infected newborns for reasons that are still largely unknown. Here, we report a comprehensive genomic analysis of 626 CC17 isolates collected worldwide, identifying the genetic traits behind thei...
In Vitro Activity of Quaternary Ammonium Surfactants against Streptococcal, Chl...
Inácio, Ângela S.; Nunes, Alexandra; Milho, Catarina; Mota, Luís Jaime; Borrego, Maria J.; Gomes, João P.; Vaz, Winchil L.C.; Vieira, Otília V.Quaternary ammonium compounds (QAC) are widely used, cheap, and chemically stable disinfectants and topical antiseptics with wide-spectrum antimicrobial activities. Within this group of compounds, we recently showed that there are significant differences between the pharmacodynamics of n-alkyl quaternary ammonium surfactants (QAS) with a short (C12) alkyl chain when in vitro toxicities toward bacterial and mamm...
Genotypes and antimicrobial resistant phenotypes of Neisseria gonorrhoeae in Po...
Florindo, Carlos; Pereira, Rui; Boura, Márcia; Nunes, Baltazar; Paulino, Albertina; Gomes, João Paulo; Borrego, Maria J.OBJECTIVES: To determine the antibiotic phenotype and MAST-genotype distribution of Neisseria gonorrhoeae isolates in Portugal between 2004 and 2009, and to evaluate specific associations between MAST-genotypes and sexual orientation, age and antibiotic resistance. METHODS: A total of 236 N gonorrhoeae isolates were typed through N gonorrhoeae multiantigen sequence typing (NG-MAST). The degree of polymorphism a...
Correlating Chlamydia trachomatis infectious load with urogenital ecological su...
Gomes, João P.; Borrego, Maria J.; Atik, Berna; Santo, Irene; Azevedo, Jacinta; Brito de Sá, Armando; Nogueira, Paulo; Dean, DeborahThe association of infectious burden of Chlamydia trachomatis with patient characteristics and clinical disease may have implications for understanding disease pathogenesis.We examined chlamydial load from 171 urine samples where load was based on copy number of organisms per copy number of eukaryotic cells derived by real-time quantitative PCR. High- (E, F, G) and low-prevalence (Ia, H, J, Ja) genotypes in the...