3 documents found, page 1 of 1
Use of ELISA based on NcSRS2 of Neospora caninumexpressed in Pichia pastoris fo...
Pinheiro,Amanda Fernandes; Borsuk,Sibele; Berne,Maria Elisabeth Aires; Pinto,Luciano da Silva; Andreotti,Renato; Roos,Talita; Roloff,Barbara CoutoNeosporosis is a disease caused by the protozoon Neospora caninum that leads to significant economic losses in many countries. In the present study, we report on use of the recombinant protein NcSRS2 of N. caninum expressed in Pichia pastoris in an indirect immunoenzymatic assay (ELISA) for diagnosing neosporosis infection in sheep and dogs. We observed that the ELISA test yielded specificity of 94.5% and sensi...
Determination of IgG avidity in BALB/c mice experimentally infected with Toxoca...
Schoenardie,Elizandra Roselaine; Scaini,Carlos James; Avila,Luciana Farias da Costa de; Sperotto,Rita Leal; Borsuk,Sibele; Felicetti,Cristine Dias PiresToxocariasis is a zoonotic disease in that IgM titers can remain high for long periods making difficult to determine the stage of the disease. The aim of this study is to investigate the applicability of indirect ELISA, associated with urea, to discriminate between the acute and chronic toxocariasis. IgG avidity was evaluated in 25 BALB/c mice experimentally infected with 1000 Toxocara canis eggs. Blood samples...
Vertical transmission of Toxocara canis in successive generations of mice
Schoenardie,Elizandra Roselaine; Scaini,Carlos James; Pepe,Michele Soares; Borsuk,Sibele; Avila,Luciana Farias da Costa de; Villela,MarcosVisceral toxocariasis is a neglected zoonosis caused by Toxocara canis larvae in unusual hosts. In dogs, the definitive host, the infection occurs mainly through transplacental and transcolostral transmission. Studies on experimental models have shown that vertical transmission may result from acute infections. Considering that toxocariasis is characterized as a chronic infection, with possible reactivation of ...