The Polyploid Gene Assembler (PGA), developed and tested in this study, represents a new strategy to perform gene-space assembly from complex genomes using low coverage DNA sequencing. The pipeline integrates reference-assisted loci and de novo assembly strategies to construct high-quality sequences focused on gene content. Pipeline validation was conducted with wheat (Triticum aestivum), a hexaploid species, u...
Agradecimentos: We thank Halley Caixeta de Oliveira and Li Yang for valuable suggestions and critical reading of the article, Marc Lohse for his assistance with the MapMan annotation, Ramon Vidal for assistance with the bioinformatics, and Ricardo Silverio Machado for helping with the photosynthesis measurements. We thank the University of North Carolina High Throughput Sequencing Facility team for intense supp...
Orientador: Vera Nisaka Solferini; Dissertação (mestrado) - Universidade Estadual de Campinas, Instituto de Biologia; Resumo: Moluscos que vivem na zona entremarés estão sujeitos a uma grande heterogeneidade ambiental, o que influencia vários aspectos de sua biologia evolutiva. Entre eles, as lapas estão entre os gastrópodes mais abundantes nos costões de todo o planeta, sendo Collisella subrugosa a única espéc...