4 documents found, page 1 of 1
Strategies to select yeast starters cultures for production of flavor compounds...
Souza, Anderson Proust Gonçalves de; Vicente, Maristela de Araújo; Klein, Raphael Contelli; Fietto, Luciano Gomes; Coutrim, Maurício XavierIn this work, we have used classical genetics techniques to find improved starter strains to produce cachaça with superior sensorial quality. Our strategy included the selection of yeast strains resistant to 5,5′,5″-trifluor-d,l-leucine (TLF) and cerulenin, since these strains produce higher levels of higher alcohols and esters than parental strains. However, no clear relationship was observed when levels of fl...
Molecular diagnosis of canine visceral leishmaniasis : a comparative study of t...
Reis, Levi Eduardo Soares; Vital, Wendel Coura; Roatt, Bruno Mendes; Bouillet, Leoneide Érica Maduro; Ker, Henrique GamaPolymerase chain reaction (PCR) and its variations represent highly sensitive and specificmethods for Leishmania DNA detection and subsequent canine visceral leishmaniasis (CVL)diagnosis. The aim of this work was to compare three different molecular diagnosis tech-niques (conventional PCR [cPCR], seminested PCR [snPCR], and quantitative PCR [qPCR])in samples of skin and spleen from 60 seropositive dogs by immun...
Clinical forms of canine visceral Leishmaniasis in naturally Leishmania infantu...
Nicolato, Roney Luiz de Carvalho; Abreu, Raquel Trópia de; Roatt, Bruno Mendes; Soares, Rodrigo Dian de Oliveira Aguiar; Reis, Levi Eduardo SoaresHematological analysis has limited applications for disease diagnosis in Leishmania infantum–infected dogs, but it can be very important in evaluating the clinical forms of the disease and in understanding the evolution of canine visceral leishmaniasis (CVL) pathogenesis. Recently, we demonstrated that alterations in leucopoiesis and erythropoiesis are related to clinical status and bone marrow parasite density...
The involvement of calcium carriers and of the vacuole in the glucose-induced c...
Bouillet, Leoneide Érica Maduro; Cardoso, Anamaria de Souza; Perovano, Eduardo; Pereira, R. R.; Ribeiro, Erica Milena de CastroPrevious work from our laboratories demonstrated that the sugar-induced activation of plasma membrane H+-ATPase in Saccharomyces cerevisiae is dependent on calcium metabolism with the contribution of calcium influx from external medium. Our results demonstrate that a glucose-induced calcium (GIC) transporter, a new and still unidentified calcium carrier, sensitive to nifedipine and gadolinium and activated by g...