3 documents found, page 1 of 1
Effects of cryoprotectants and low temperatures on hatching and abnormal embryo...
Costa,Raphael S.; Souza,Fabrício M. S. de; Senhorini,José A.; Veríssimo-Silveira,Rosicleire; Ninhaus-Silveira,AlexandreABSTRACT This study evaluated the effect of the cryoprotectants and the low temperatures on the embryonic development of Prochilodus lineatus, describing their main morphological alterations. On chilling sensitivity test, the survival rates at the twenty somites stage (20S) were 53.6% at 0ºC, and 100% in 5ºC. To test toxicity, the embryos were exposed to a graded series of 1,2-Propanediol (PROP), dimethyl sulfo...
Cytogenetic characterization of the strongly electric Amazonian eel, Electropho...
Fonteles,Soraia B.A.; Lopes,Carlos E.; Akama,Alberto; Fernandes,Flora M.C.; Porto-Foresti,Fábio; Senhorini,José A.; Daniel-Silva,Maria de Fátima Z.A karyotype analysis of the electric eel, Electrophorus electricus (Teleostei, Gymnotiformes), a strongly electric fish from northern South America, is presented. Two female specimens were analyzed, one from the Amazon River and one from the Araguaia River. The specimens had a chromosomal number of 2n = 52 (42M-SM + 10A). C-bands were present in a centromeric and pericentromeric position on part of the chromoso...
Paraoxonase activity in liver of Pacu, Piaractus mesopotamicus Holmberg (Charac...
Bastos,Vera Lucia F. Cunha; Folly,Evelize; Rossini,Ana; Ceccarelli,Paulo S.; Senhorini,José A.; Bastos,Jayme CunhaEnzymatic production of p-nitrophenol in liver of Piaractus mesopotamicus Holmberg, 1887 was consistently assayed at pH 8.5 using 7.5 mM paraoxon as substrate. This pacu liver paraoxonase activity was activated by NaCl. Apparent values of K M were 2.42 x 10-3 M in the presence of 0.5 M NaCl and 8.99 x 10-3 M without NaCl. Apparent maximum velocity values calculated in the absence and presence of 0.5 M NaCl were...