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Density separation of petrous bone powders for optimized ancient DNA yields

Fernandes, Daniel; Sirak, Kendra A.; Cheronet, Olivia; Novak, Mario; Brück, Florian; Zelger, Evelyn; Llanos-Lizcano, Alejandro; Wagner, Anna

Density separation is a process routinely used to segregate minerals, organic matter, and even microplastics, from soils and sediments. Here we apply density separation to archaeological bone powders before DNA extraction to increase endogenous DNA recovery relative to a standard control extraction of the same powders. Using nontoxic heavy liquid solutions, we separated powders from the petrous bones of 10 indi...

Date: 2023   |   Origin: Estudo Geral - Universidade de Coimbra
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