22 documents found, page 1 of 3
Improving fructooligosaccharide production via sacC gene deletion in Zymomonas ...
Braga, Adelaide Correia; Maia, Ana Benedita; Gomes, Daniela Filipa Correia; Rodrigues, Joana Lúcia Lima Correia; Costa, João Manuel RainhaFructooligosaccharides (FOS) are promising prebiotics in the relevant and increasing market of functional food. Industrially, these compounds are produced from sucrose by the action of fructosyltransferase or b-fructofuranosidase enzymes. However, this process often yields low conversion rates and results in impure mixtures due to the release of high levels of glucose. Zymomonas mobilis is a well-known ethanol-...
Be@t project: valorisation of waste cooking oil and grapevine pruning for susta...
Rainha, João; Braga, Adelaide Correia; Cardoso, Beatriz B.; Fernandes, Margarida M; Rodrigues, Catarina Cunha; Santos, Maria Anita CarvalhoThe development of sustainable alternatives to synthetic surfactants is increasingly important for reducing environmental impact in industries such as textiles. Rhamnolipids, glycolipid biosurfactants produced by Pseudomonas aeruginosa, combine excellent surface activity, biodegradability, and versatility. However, the high production costs, largely due to substrate expenses, limit their commercial use. The Be@...
Be@t project: bioconversion of agro-industrial wastes into biosurfactants for t...
Rainha, João; Braga, Adelaide Correia; Cardoso, Beatriz B.; Fernandes, Margarida M; Rodrigues, Catarina Cunha; Santos, Maria Anita CarvalhoIntroduction: Developing sustainable alternatives to synthetic surfactants is essential to reduce the environmental impact of industries such as textiles. Rhamnolipids, glycolipid biosurfactants produced by Pseudomonas aeruginosa, combine excellent surface activity, biodegradability, and functional versatility. However, their large-scale application remains limited by high production costs, primarily due to exp...
Utilizing waste cooking oil and agro-industrial residues for cost-effective rha...
Rainha, João; Braga, Adelaide Correia; Fernandes, Margarida M; Rodrigues, Catarina Cunha; Silva, Carla Alexandra Pereira; Rodrigues, L. R.Surfactants are chemical agents that reduce surface or interfacial tension between phases, widely applied in detergents, emulsifiers, and personal care products. Petrochemical surfactants dominate a market worth over 40 billion, but their production contributes to greenhouse gas emissions, and their disposal causes significant water and soil pollution. Biosurfactants, synthesized by microorganisms, offer a sust...
Agro-industrial by-products valorization for fructooligosaccharide production w...
Braga, Adelaide Correia; Maia, Ana Benedita; Rodrigues, L. R.Fructooligosaccharides (FOS) have gained attention due to their prebiotic properties and potential health benefits. This study explores the production of FOS using Zymomonas mobilis ZM4, a promising candidate for biotechnological processes, utilizing corn steep liquor (CSL) and sugarcane molasses as alternative and sustainable carbon and nitrogen sources. Two distinct media formulations were investigated, namel...
Optimization of chondroitin production in E. coli using genome scale models
Couto, Márcia R.; Rodrigues, Joana Lúcia Lima Correia; Braga, Adelaide Correia; Dias, Oscar; Rodrigues, L. R.Chondroitin is a natural occurring glycosaminoglycan with applications as a nutraceutical and pharmaceutical ingredient and can be extracted from animal tissues. Microbial chondroitin-like polysaccharides emerged as a safer and more sustainable alternative source. However, chondroitin titers using either natural or recombinant microorganisms are still far from meeting the increasing demand. The use of genome-sc...
Agro-industrial wastes as alternative substrates for the production of prebioti...
Braga, Adelaide Correia; Maia, Ana Benedita; Gomes, Daniela Filipa Correia; Costa, João Manuel Rainha; Cardoso, Beatriz Alexandra BatistaFructooligosaccharides (FOS) are promising prebiotics in the relevant and increasing market of functional food. However, to achieve a more sustainable process, the industrial production of FOS should use cheap substrates. Zymomonas mobilis (ZM) has great potential for the production of FOS due to the presence of native enzymes (levansucrase) capable of metabolizing sucrose. In addition, ZM can use different car...
Atmospheric plasma and UV polymerisation for developing sustainable anti-adhesi...
Caykara, Tugce; Fernandes, Sara; Braga, Adelaide Correia; Rodrigues, Joana Lúcia Lima Correia; Rodrigues, L. R.; Silva, Carla JoanaEnhancing the hydrophilicity of polymeric materials is an important step for achieving anti-adhesiveness. Thus, in this study, atmospheric plasma as a pre-treatment was combined with a UV grafting process to obtain a durable surface modification on polyethylene terephthalate (PET). The most promising conditions for the atmospheric plasma process were found to be 15 kW power and 4 m/min speed, leading to a conta...
Design of new hydrolyzed collagen-modified magnetic nanoparticles to capture pa...
Sande, Maria Georgina; Roque, Lúcia; Braga, Adelaide Correia; Marques, Márcia; Ferreira, Débora Carina Gonçalves Abreu; Saragliadis, AthanasiosEnrichment and diagnosis tools for pathogens currently available are time consuming, thus the development of fast and highly sensitive alternatives is desirable. In this study, a novel approach was described that enables selective capture of bacteria expressing hydrolyzed collagen-binding adhesins with hydrolyzed collagen-coated magnetic nanoparticles (MNPs). This platform could be useful to shorten the time ne...
Two-step purification of epilactose produced by cellobiose 2-epimerase from Cal...
Cardoso, Beatriz B.; Fernandes, Jean Michel; Pinheiro, Ana C.; Braga, Adelaide Correia; Silvério, Sara Isabel Cruz; Rodrigues, L. R.Epilactose is a functional sugar that can be produced from lactose using cellobiose 2-epimerase and it is considered a developing prebiotic. In that sense, the development of strategies to produce and purify epilactose is key for its wider use in the food industry. The aim of this work was to establish a food-grade purification strategy suitable to be scaled-up to an industrial level. Firstly, the epilactose wa...