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Photochemical treatment strategies for okadaic acid degradation: Effects of sal...

Moreno-Andrés, Javier; Lage, Sandra; Braga, Ana Catarina; Reis Costa, Pedro

This study evaluates the degradation and detoxification of okadaic acid (OA), a marine biotoxin, through UVassisted photochemical processes using environmentally relevant OA concentrations. Experiments were conducted in distilled water (DW) and artificial seawater (ASW), applying two UV sources: UV-LED (lambda(max) = 275 nm) and low-pressure mercury lamp (LP-Hg; lambda = 254 nm), combined with hydrogen peroxide...


Lipophilic marine toxins in sediments from Arrábida marine protected area, Port...

Soliño, Lucia; Braga, Ana Catarina; Lobo-Arteaga, Jorge; Reis Costa, Pedro

During the development and senescence of harmful algal blooms (HAB), most of the algae cells not ingested by grazers or filter-feeding organisms sink to the bottom, making sediments important reservoirs of algae toxins. In this study, lipophilic marine toxins were determined in the sediments collected from depths ranging from 5 to 145 m depth in the marine protected area of Arrabida (southwest Portuguese coast)...


Lipophilic marine toxins in sediments from Arrábida marine protected area, Port...

Soliño, Lucia; Braga, Ana Catarina; Lobo-Arteaga, Jorge; Reis Costa, Pedro

During the development and senescence of harmful algal blooms (HAB), most of the algae cells not ingested by grazers or filter-feeding organisms sink to the bottom, making sediments important reservoirs of algae toxins. In this study, lipophilic marine toxins were determined in the sediments collected from depths ranging from 5 to 145 m depth in the marine protected area of Arrabida ´ (southwest Portuguese coas...


Gymnodinium catenatum paralytic Shellfish toxin production and photobiological ...

Lopes, Vanessa M.; Court, Mélanie; Seco, Martim Costa; Borges, Francisco O.; Vicente, Bernardo; Lage, Sandra; Braga, Ana Catarina; Duarte, Bernardo

Marine heatwaves (MHWs) have doubled in frequency since the 1980s and are projected to be exacerbated during this century. MHWs have been shown to trigger harmful algal blooms (HABs), with severe consequences to marine life and human populations. Within this context, this study aims to understand, for the first time, how MHWs impact key biological and toxicological parameters of the paralytic shellfish toxin (P...


Bivalve shellfish safety in Portugal: variability of faecal levels, metal conta...

Braga, Ana Catarina; Rodrigues, Susana Margarida; Lourenço, Helena Maria; Reis Costa, Pedro; Pedro, Sónia

Bivalves are a high-value product whose production has markedly increased, reaching 9863 tonnes in Portugal in 2021. Bivalves' habitats-lagoons, estuaries and coastal waters-are exposed to biological and anthropogenic contaminants, which can bioaccumulate in these organisms and pose a significant public health risk. The need to obtain a safe product for human consumption led to the implementation of standardise...


Does Ocean Sunfish Mola spp. (Tetraodontiformes: Molidae) represent a risk for ...

Baptista, Miguel; Braga, Ana Catarina; Rosa, Rui; Reis Costa, Pedro

Tetrodotoxin (TTX) is a potent neurotoxin naturally occurring in terrestrial and marine organisms such as pufferfish. Due to the risk of TTX poisoning, fish of Tetraodontidae family and other puffer-related species must not be placed in the EU markets. This restriction applies to fish of the family Molidae even though no data on toxins’ occurrence is available. In this study, the presence of TTX and its analogu...


Bioaccessibility of lipophilic and hydrophilic marine biotoxins in seafood: an ...

Alves, Ricardo N.; Rambla-Alegre, Maria; Braga, Ana Catarina; Maulvault, Ana L.; Barbosa, Vera; Campàs, Mònica; Reverté, Laia; Flores, Cintia

This study aimed to assess the bioaccessibility of different marine biotoxins in naturally contaminated shellfish and fish gonads using an in vitro digestion methodology. In general, hydrophilic toxins (domoic acid, paralytic shellfish poisoning toxins and tetrodotoxins) showed higher bioaccessibility than lipophilic ones (okadaic acid and azaspiracids). The bioaccessibility of toxins from the okadaic acid grou...


Bioaccessibility of lipophilic and hydrophilic marine biotoxins in seafood: an ...

Alves, Ricardo N.; Rambla-Alegre, Maria; Braga, Ana Catarina; Maulvault, Ana L.; Barbosa, Vera; Campàs, Mònica; Reverté, Laia; Flores, Cintia

This study aimed to assess the bioaccessibility of different marine biotoxins in naturally contaminated shellfish and fish gonads using an in vitro digestion methodology. In general, hydrophilic toxins (domoic acid, paralytic shellfish poisoning toxins and tetrodotoxins) showed higher bioaccessibility than lipophilic ones (okadaic acid and azaspiracids). The bioaccessibility of toxins from the okadaic acid grou...


In vitro bioaccessibility of the marine biotoxins okadaic acid, dinophysistoxin...

Manita, Diana; Alves, Ricardo N.; Braga, Ana Catarina; Fogaca, Fabiola H. S.; Marques, Antonio; Reis Costa, Pedro

Okadaic acid (OA), Dinophysistoxins (DTX1 and DTX2) and their acyl-derivatives (DTX3) are marine toxins responsible for the human diarrhetic shellfish poisoning. To date the amount of toxins ingested from consumption of shellfish has been considered equal to the amount of toxins available for uptake by the human body. The aim of this study is to assess the OA, DTX2 and DTX3 fractions released from raw and steam...


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