11 documents found, page 1 of 2
OmniSARS2: A Highly Sensitive and Specific RT-qPCR-Based COVID-19 Diagnostic Me...
Carvalho-Correia, Eduarda; Calçada, Carla; Branca, Fernando; Estévez-Gómez, Nuria; De Chiara, Loretta; Varela, Nair; Gallego-García, PilarExtensive transmission of SARS-CoV-2 during the COVID-19 pandemic allowed the generation of thousands of mutations within its genome. While several of these become rare, others largely increase in prevalence, potentially jeopardizing the sensitivity of PCR-based diagnostics. Taking advantage of SARS-CoV-2 genomic knowledge, we designed a one-step probe-based multiplex RT-qPCR (OmniSARS2) to simultaneously detec...
OmniSARS2: a highly sensitive and specific RT-qPCR-based COVID-19 diagnostic me...
Carvalho-Correia, Eduarda; Calçada, Carla Sofia Martins; Branca, Fernando; Estévez-Gómez, Nuria; De Chiara, Loretta; Varela, Nair; Gallego-García, PilarExtensive transmission of SARS-CoV-2 during the COVID-19 pandemic allowed the generation of thousands of mutations within its genome. While several of these become rare, others largely increase in prevalence, potentially jeopardizing the sensitivity of PCR-based diagnostics. Taking advantage of SARS-CoV-2 genomic knowledge, we designed a one-step probe-based multiplex RT-qPCR (OmniSARS2) to simultaneously detec...
Sampling Methods and Risk Stratification Regarding Environmental Contamination ...
Mendes, Marta; Andrade Oliveira, Ana; Pires, Olga; Branca, Fernando; Beirão, Maria; Santa-Cruz, André; Carvalho, Alexandre; Alves, JoanaIntroduction: Transmission of COVID-19 through close contact and droplets is well established, but the influence of aerosol and surface contamination remains to be determined. Literature is scarce and inconsistent about the viable virus particles free-distance from infected patients, as well as about different swabbing methods for surface contamination evaluation. The aim of this study was to evaluate the most ...
Acute alithiasic cholecystitis and human herpes virus type-6 infection: first case
Gomes, Maria Miguel; Antunes, Henedina; Lobo, Ana Luísa; Branca, Fernando; Pinto, Jorge Correia; Pinto, João MoreiraA three-year-old male child presented with erythematous maculopapular nonpruritic generalized rash, poor feeding, vomiting, and cramping generalized abdominal pain. He was previously healthy and there was no family history of immunologic or other diseases. On examination he was afebrile, hemodynamically stable, with painful palpation of the right upper quadrant and positive Murphy's sign. Laboratory tests revea...
Analysis of a Local HIV-1 Epidemic in Portugal Highlights Established Transmiss...
Carvalho, Alexandre; Costa, Patrício Soares; Triunfante, Vera; Branca, Fernando; Rodrigues, Fernando; Santos, Catarina L.; Correia-Neves, MargaridaThe existing data supports Portugal as the Western Europe country with highest HIV-1 subtype diversity. However, detailed phylogenetic studies of Portuguese HIV-1 epidemics are still scarce. Thus, our main goal was to analyze the phylodynamics of a local HIV-1 infection in the Portuguese region of Minho. Molecular epidemiological analysis was applied to data from 289 HIV-1 infected individuals followed in the r...
High level biosynthesis of a silk-elastin-like protein in E. coli
Collins, Tony; Barroca, Mário Jorge Faria; Branca, Fernando; Padrão, Jorge; Machado, Raul; Casal, MargaridaSilk-elastin-like proteins (SELPs) have enormous potential for use as customizable biomaterials in numerous biomedical and materials applications yet success in harnessing this potential has been limited by the lack of a commercially viable industrially relevant production process. We have developed a scalable fed-batch production approach which enables a SELP volumetric productivity of 4.3 g L-1 with E. coli B...
Optimising the production of a silk-elastin-like protein in E. coli: overcoming...
Collins, Tony; Barroca, Mário Jorge Faria; Branca, Fernando; Silva, João Azevedo; Costa, André da; Machado, Raul; Casal, MargaridaSilk-elastin-like proteins (SELPs) combining the physicochemical and biological properties of silk and elastin have a high potential for use in the pharmaceutical, regenerative medicine and materials fields. Their development for use is however restrained by their production levels. Here we describe the production optimisation for a novel recently described SELP in the pETE. coli BL21(DE3) expression system. Bo...
Key stress factors and parameters for production optimisation of silk-elastin-l...
Collins, Tony; Silva, João Azevedo; Costa, André da; Branca, Fernando; Machado, Raul; Casal, MargaridaSilk-elastin-like proteins (SELPs) combining the physicochemical and biological properties of silk and elastin have a high potential for use in the pharmaceutical, regenerative medicine and materials fields. Their development for use is however restrained by their production levels. We have recently synthesised a series of novel silk-elastin-like proteins and here we will describe the optimisation of the produc...
Batch production of a silk-elastin-like protein in E. coli BL21(DE3) : key para...
Collins, Tony; Silva, João Azevedo; Costa, André da; Branca, Fernando; Machado, Raul; Casal, MargaridaBackground: Silk-elastin-like proteins (SELPs) combining the physicochemical and biological properties of silk and elastin have a high potential for use in the pharmaceutical, regenerative medicine and materials fields. Their development for use is however restrained by their production levels. Here we describe the batch production optimisation for a novel recently described SELP in the pET-E. coli BL21(DE3) ex...
Optimising a cell factory system for the bioproduction of silk-elastin-like pol...
Collins, Tony; Barroca, Mário Jorge Faria; Branca, Fernando; Silva, João Azevedo; Costa, André da; Machado, Raul; Casal, MargaridaSilk-elastin-like proteins (SELPs) combining the physicochemical and biological properties of silk and elastin have a high potential for use in the pharmaceutical, regenerative medicine and materials fields. Their development for use is however restrained by their production levels. Here we describe the production optimisation for a novel recently described SELP in the pETE. coli BL21(DE3) expression system. Bo...