ABSTRACT:Biotechnological active peptides are gaining interest in the cosmetics industry due to their antimicrobial, anti-inflammatory, antioxidant, and anti-collagenase (ACE) effects, as well as wound healing properties, making them suitable for cosmetic formulations. The antimicrobial activity of peptides (2–10 kDa) secreted by Saccharomyces cerevisiae Ethanol-Red was evaluated against dermal pathogens using ...
ABSTRACT: The rising demand for minimally processed, natural, and healthier food products has led to the search for alternative and multifunctional bioactive food components. Therefore, the present study focuses on the functional proprieties of a peptide fraction derived from Saccharomyces cerevisiae metabolism. The antimicrobial activity of the peptide fraction is evaluated against various foodborne pathogens,...
Sulfite is a common preservative in wine, but the spoilage yeast Brettanomyces bruxellensis can produce volatile phenols even with the recommended sulfite dose. The purpose of this study was to examine how wine components, p-coumaric acid (a precursor of volatile phenols), and micronutrients influence culturability, viability, and volatile phenols production by B. bruxellensis under sulfite stress. In red wine,...
ABSTRACT: Lactic acid bacteria (LAB) and Brettanomyces bruxellensis are the main contaminants of bioethanol fermentations. Those contaminations affect Saccharomyces cerevisiae performance and reduce ethanol yields and productivity, leading to important economic losses. Currently, chemical treatments such as acid washing and/or antibiotics are used to control those contaminants. However, these control measures c...
ABSTRACT: Winemaking involves a wide diversity of microorganisms with different roles in the process. The wine microbial consortium (WMC) includes yeasts, lactic acid bacteria and acetic acid bacteria with different implications regarding wine quality. Despite this technological importance, their origin, prevalence, and routes of dissemination from the environment into the winery have not yet been fully unravel...
ABSTRACT: The yeast Brettanomyces bruxellensis is one of the most dangerous wine contaminants due to the production of phenolic off-flavors such as 4-ethylphenol. This microbial hazard is regularly tackled by addition of sulfur dioxide (SO2). Nevertheless, B. bruxellensis is frequently found at low levels (ca 10(3) cells/mL) in finished wines. Besides, consumers health concerns regarding the use of sulfur dioxi...
Submitted by DSpace Unilasalle (dspace@unilasalle.edu.br) on 2020-11-17T16:44:33Z No. of bitstreams: 1 pbranco.pdf: 2702282 bytes, checksum: 5d3ba307a377d09faceb3a93277583ff (MD5); Made available in DSpace on 2020-11-17T16:44:33Z (GMT). No. of bitstreams: 1 pbranco.pdf: 2702282 bytes, checksum: 5d3ba307a377d09faceb3a93277583ff (MD5) Previous issue date: 2020; A presente Dissertação traz a reflexão das questões ...
This work was focused on: i) the investigation of the autofluorescence of various materials that are found forming part of Cultural Heritage objects (parchment, paper, wood, fabric, rock, mortar and plastic among others) by epifluorescence microscopy using fluorescence flter sets targeted at commonly used fluorophores (TRITC/CY5/6-FAM); ii) the detection of RNA-FISH stained microbial cells on the selected materials...
This work was focused on the design of an RNA-FISH probe target to a yeast specie (Dekkera/Brettanomyces bruxellensis) and on the evaluation of its suitability in silico and experimentally; FCT through the project PTDC/BBB-IMG/0046/2014 and grant SFRH/BPD/100754/2014 and by ALT20-03-0246-FEDER-000004-ALENTEJO 2020 project
The aim of this work was to investigate the possibility of applying the RNA-FISH protocol previously developed by our group for simultaneous analysis of fungi and bacteria using kingdom-specific probes for specific detection of various yeast species using previously published species-specific probes. Their specificity and performance were analysed both in silico and experimentally.; FCT through PTDC/BBB-IMG/004...