7 documents found, page 1 of 1
Anti-serum validation for use in immunohistochemistry for Trypanosoma cruzi det...
Azevedo, Paulo Hernane Rabelo; Xavier, Marcelo Antônio Pascoal; Silva, Glenda Nicioli da; Costa, Priscilla Almeida da; Carneiro, Cláudia MartinsIntroduction: The detection of Trypanosoma cruzi in tissue samples is important in many situations, such as testing of the reactivation of the infection. The detection of T. cruzi nests in endomyocardial biopsies (EMB) may be useful to evaluate graft rejection. Given their scarcity, such nests are not routinely identified. To increase the diagnosis sensitivity, immunohistochemistry (IHC) may serve as a promisin...
Early polymerase chain reaction detection of Chagas disease reactivation in hea...
Costa, Priscilla Almeida da; Segatto, Marcela [UNESP]; Durso, Danielle Fernandes; Carvalho Moreira, Wagson Jose de; Junqueira, Lucas LodiMade available in DSpace on 2018-11-26T15:44:34Z (GMT). No. of bitstreams: 0 Previous issue date: 2017-07-01; Fundação de Amparo à Pesquisa do Estado de Minas Gerais (FAPEMIG); Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq); Departamento de Ciencia e Tecnologia do Ministerio da Saude (Decit/SCTIE/MS); Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES); BACKGROUND: Heart tr...
Correlation of endoscopic and histological features in adults with suspected ce...
Rosa, Rodrigo Macedo; Ferrari, Maria de Lourdes Abreu; Pedrosa, Moisés Salgado; Ribeiro, Gustavo Meirelles; Brasileiro Filho, GeraldoContext - Clinical presentation of celiac disease is extremely variable and the diagnosis relies on serologic tests, mucosal intestinal biopsy and clinic and serologic response to a gluten-free diet. Objectives - To correlate the endoscopic and histological aspects of adult patients with suspicion of celiac disease and to evaluate the interobserver histological agreement. Method - Endoscopic aspects of 80 adult...
Bracken fern-induced malignant tumors in rats : absence of mutations in p53, H-...
Freitas, Renata Nascimento de; Brasileiro Filho, Geraldo; Silva, Marcelo Eustáquio; Pena, Sérgio Danilo JunhoBracken fern (genus Pteridium) has been shown to induce tumors in domestic and experimental animals. Epidemiological studies have also shown an association between human exposure to bracken toxins and increased risk for the development of upper gastrointestinal tract tumors. Our aim in this study was to investigate possible genomic alterations in bracken fern-induced tumors of experimental animals searching for...
Genetic profiling of Trypanosoma cruzi directly in infected tissues using neste...
Pimenta, Juliana Ramos; Freitas, Jorge Marcelo de; Duffy, Tomás; Bartholomeu, Daniella Castanheira; Oliveira, Riva de Paula; Chiari, EglerThe investigation of the importance of the genetics of Trypanosoma cruzi in determining the clinical course of Chagas disease will depend on precise characterisation of the parasites present in the tissue lesions. This can be adequately accomplished by the use of hypervariable nuclear markers such as microsatellites. However the unilocal nature of these loci and the scarcity of parasites in chronic lesions make...
Changes in pentobarbital induced sleeping-time in mice infected with Schistosom...
Tonelli, Sônia Maria de Assis Fonseca; Brasileiro Filho, Geraldo; Goulart, Eugênio Marcos de Andrade; Coelho, Paulo Marcos ZechPara avaliar se a intensidade da reação granulomatosa hepática induzida por ovos de S. mansoni tem relação com o metabolismo de drogas, camundongos foram infectados com 40 cercárias e testados quanto ao tempo de anestesia pelo pentobarbital sódico. Para diminuir a resposta inflamatória usou-se irradiação com 400 Rad ou azatioprina na dose de 20mg/kg, 3 vezes por semana, por 4 semanas, respectivamente no ou a pa...
Human papillomavirus detection in cervical dysplasias or neoplasias and in cond...
Guimarães, Eliane Machado; Brasileiro Filho, Geraldo; Pena, Sérgio Danilo JunhoSpecimens from cervical dysplasias or carcinomas and genital condylomata acuminata were retrospectively analysed by in situ hybridization (ISH) with bioti-nylated DNA probes for human papillomavirus (HPV) types 6, 11, 16 and 18. In the control group no case was positive for HPV DNA. In mild/moderate dysplasias, 4 cases (14%) were positive for HPV 6 or 11 and 2 cases (7%), for HPV 16. In the severe dysplasia/in ...